博士（工学）, 大阪大学

Electron transfer in DNA, DNA damage, Artificial nucleic acid, Liquid crystalline DNA, Phasing biology

Nanotechnology/Materials, Nanobioscience

Nanotechnology/Materials, Biochemistry

Feb. 2021 - Present
電気通信大学大学院情報理工学研究科, 准教授

Feb. 2016 - Jan. 2021
電気通信大学大学院情報理工学研究科, 助教

Apr. 2013 - Jan. 2016
日本学術振興会特別研究員（RPD）

Jul. 2012 - Mar. 2013
筑波大学, 博士研究員

Apr. 2010 - Jun. 2012
日本大学工学部, 博士研究員

Apr. 2007 - Mar. 2010
日本学術振興会特別研究員（PD）

Sep. 2007 - Mar. 2009
カリフォルニア工科大学, 博士研究員（学振海外渡航）

Apr. 2005 - Mar. 2007
日本学術振興会特別研究員（DC2）

Mar. 2007
Osaka University, 工学研究科, 物質・生命工学専攻

Mar. 2004
Osaka University, 理学研究科, 化学専攻

Mar. 2002
Osaka University, 理学部, 化学科

四天王寺高等学校

Sep. 2024
第18回バイオ関連化学シンポジウム
Organic & Biomolecular Chemistry 賞, 牧野 哲直;田仲 真紀子

Jul. 2024
第35回 生体機能関連化学部会若手の会
Chemistry Letters Young Scientist Award, 牧野 哲直;田仲 真紀子

Oct. 2023
第13回CSJ化学フェスタ2023
優秀ポスター発表賞, 牧野 哲直;田仲 真紀子

Mar. 2006
日本化学会第86春季年会学生講演賞

Jan. 2005
The 5th International 21 Century COE Symposium on Integrated EcoChemistry ポスタープレゼンテーション賞

Controlling the Hierarchical Assembly of DNA-Based Hexagonal Microstructures.
Tetsunao Makino; Takashi Kajitani; Makiko Tanaka
Last, Small (Weinheim an der Bergstrasse, Germany), e2410243, 23 Dec. 2024, Peer-reviwed, True, This paper discusses the controlled morphology of hierarchical liquid crystalline DNA assemblies. Through a process of heating and slow cooling, double-stranded DNAs (dsDNAs) having 23 complementary bases and two base overhangs (a pair of 25mer oligonucleotides) spontaneously assemble into micro-sized hexagonal platelets in a solution containing poly(ethylene glycol) (PEG) and salt. Remarkably, the addition of a shorter dsDNA with AA/TT overhangs (a pair of 18mer oligonucleotides) to a PEG-salt solution of 25mer DNA with AA/TT overhangs results in the formation of molecular tubes, each with a central blockage. In contrast, the addition of 18mer DNA with GG/CC overhangs leads to the formation of hexagonal frames from hexagonal platelets. In the assemblies, 18mer DNA is more predominant at the edges, while the central nuclei are composed of 25mer DNA. X-ray scattering measurements reveal that both molecular tubes and hexagonal frames form hexagonal columnar liquid crystalline assemblies. These self-assemblies are thought to result from depletion attraction due to PEG and end-to-end stacking between dsDNA overhangs. Differences in the melting temperatures between longer and shorter dsDNAs enable the stepwise formation of hierarchical assemblies, while variations in overhang sequences modulate the overall morphology by altering the growth direction of the assemblies.
Scientific journal, English

Various Forms of Self-Assembled DNA and Their Properties
Tetsunao Makino; Makiko Tanaka
Last, Accounts of Materials & Surface Research, 8, 2, 107-115, Jun. 2023, Peer-reviwed, Invited

Self-assembled micro-sized hexagons built from short DNA in a crowded environment
Tetsunao Makino; Daisuke Nakane; Makiko Tanaka
Last, ChemBioChem, Wiley-VCH, 23, e202200360, Oct. 2022, Peer-reviwed
Scientific journal, English

Evaluation of DNA-mediated electron transfer using a hole-trapping nucleobase under crowded conditions
Yuuki Taketomi; Yuuki Yamaguchi; Shunsuke Sakurai; Makiko Tanaka
Last, Organic & Biomolecular Chemistry, 20, 2043-2047, 10 Mar. 2022, Peer-reviwed
Scientific journal, English

Guanine damage by singlet oxygen from SYBR Green I in liquid crystalline DNA
Sakurai, S; Jo, K; Kinoshita, H; Esumi, M; Tanaka, M
Last, Organic & Biomolecular Chemistry, Royal Society of Chemistry, 18, 7183-7187, 04 Sep. 2020, Peer-reviwed
Scientific journal, English

Effect of spermidine on guanine decomposition via photoinduced electron transfer in DNA
Mayu Esumi; Shunsuke Sakurai; Makiko Tanaka
Last, Organic & Biomolecular Chemistry, Royal Society of Chemistry, 18, 47-51, Jan. 2020, Peer-reviwed
Scientific journal, English

Drastic promotion of guanine oxidation via electron transfer in Ψ-type DNA
Shunsuke Sakurai; Mayu Esumi; Makiko Tanaka
Last, Chemical Communications, 55, 7695-7698, Jun. 2019, Peer-reviwed
Scientific journal, English

Attenuation of guanine oxidation via DNA-mediated electron transfer in crowded environment using small cosolutes
Makiko Tanaka; Takayuki Matsumoto; Hiroki Iida
Lead, Organic & Biomolecular Chemistry, Royal Society of Chemistry, 16, 6695-6702, Sep. 2018, Peer-reviwed
Scientific journal, English

Effects of molecular-crowding on electron transfer and oxidative damage in pyrene-modified oligonucleotides
Makiko Tanaka; Hiroki Iida; Takayuki Matsumoto
Lead, Chemistry Letters, Chemical Society of Japan, 47, 1, 62-64, 2018, Peer-reviwed, We examined guanine damages caused by photoinduced electron transfer (ET) in biomimetic environments using pyrene-modified oligonucleotides and molecular crowders. As a result, guanine damage induced by ET in full-matched DNA was decreased under viscous crowding conditions. On the other hand, behaviors of mismatched DNA revealed that through-space ET between pyrene moiety and the consecutive guanine site was promoted under molecular-crowding conditions.
Scientific journal, English

Ribonuclease A as Effective Promoter for Unimolecular Invasion of Peptide Nucleic Acid to Double-stranded DNA
Makiko Tanaka; Narumi Shigi; Makoto Komiyama
Lead, CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 45, 7, 767-769, Jul. 2016, Peer-reviwed, With the use of ribonuclease A (RNase A) as promoter, only one strand of peptide nucleic acid (PNA) efficiently invades double-stranded DNA (dsDNA), according to the Watson Crick rule. The RNase A locally rewinds the dsDNA to facilitate unimolecular invasion, which otherwise hardly occurs. Furthermore, the single-stranded portion, formed at the invasion site in the dsDNA, is trapped by the protein.
Scientific journal, English

Thiazole orange-conjugated peptide nucleic acid for fluorescent detection of specific DNA sequences and site-selective photodamage
Makiko Tanaka; Narumi Shigi; Jun Sumaoka; Makoto Komiyama
Lead, RSC ADVANCES, ROYAL SOC CHEMISTRY, 4, 108, 63533-63538, 2014, Peer-reviwed, A fluorescent probe to detect specific sequences in double-stranded DNA (dsDNA) has been synthesized by conjugating thiazole orange (TO) with pseudo-complementary peptide nucleic acid (pcPNA). A pair of TO-conjugated pcPNAs bound to the target site in dsDNA through double-duplex invasion, and promoted intercalation of the TO moieties for notable enhancement of their fluorescence. Site-specificity of the detection is high enough to differentiate only one base-pair difference in DNA. These TO-pcPNA conjugates also function as site-selective photo-damage inducers. Upon irradiation with visible light, singlet oxygen was generated by energy transfer from the dyes at the invasion site, resulting in the site-selective formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxoG) there.
Scientific journal, English

Fluorometric detection of adenine in target DNA by exciplex formation with fluorescent 8-arylethynylated deoxyguanosine
Yoshio Saito; Kenji Kugenuma; Makiko Tanaka; Azusa Suzuki; Isao Saito
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON-ELSEVIER SCIENCE LTD, 22, 11, 3723-3726, Jun. 2012, Peer-reviwed, We demonstrated an intriguing method to discriminate adenine by incident appearance of an intense new emission via exciplex formation in hybridization of target DNA with newly designed fluorescent 8-arylethynylated deoxyguanosine derivatives. We described the synthesis of such highly electron donating fluorescent guanosine derivatives and their incorporation into DNA oligomers which may be used for the structural study and the fluorometric analysis of nucleic acids. (C) 2012 Elsevier Ltd. All rights reserved.
Scientific journal, English

Enhancement of fluorescence quenching and exciplex formation in DNA major groove by double incorporation of modified fluorescent deoxyuridines
Makiko Tanaka; Kazuhiro Oguma; Yoshio Saito; Isao Saito
Lead, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON-ELSEVIER SCIENCE LTD, 22, 12, 4103-4105, Jun. 2012, Peer-reviwed, 5-(1-Naphthalenylethynyl)-2'-deoxyuridine (U-N) and 5-[ (4-cyano-1-naphthalenyl) ethynyl]-2'-deoxyuridine (U-CN) were synthesized and incorporated into oligodeoxynucleotides. Fluorescence emissions of modified duplexes containing double U-N were efficiently quenched depending upon the sequence pattern of the naphthalenes in DNA major groove, as compared to the duplex possessing single U-N. When one of the naphthalene moieties has a cyano substituent, the exciplex emission from the chromophores in DNA major groove was observed at longer wavelength. (C) 2012 Elsevier Ltd. All rights reserved.
Scientific journal, English

Drastic enhancement of excess electron-transfer efficiency through DNA by inserting consecutive 5-phenylethynyl-2 '-deoxyuridines as a modulator
Makiko Tanaka; Kazuhiro Oguma; Yoshio Saito; Isao Saito
Lead, CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 48, 75, 9394-9396, 2012, Peer-reviwed, 5-(Phenylethynyl)-2'-deoxyuridine (U-Ph) was consecutively incorporated into oligodeoxynucleotides as an electron transport modulator for regulating DNA-mediated excess electron transfer (EET). Upon photoexcitation of the ethynylpyrene chromophore, extremely high EET efficiencies through DNA containing consecutive (Ph)Us were observed using 5-bromouracil as a distal electron trap.
Scientific journal, English

Stabilization of DNA duplex by 2-substituted adenine as a minor groove modifier
Makiko Tanaka; Ryota Kozakai; Yoshio Saito; Isao Saito
Lead, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON-ELSEVIER SCIENCE LTD, 21, 23, 7021-7024, Dec. 2011, Peer-reviwed, 2-(1-Naphthalenylethynyl)-2'-deoxyadenosine ((N)A) was synthesized and incorporated into oligodeoxynucleotides. DNA duplexes containing newly designed 5'-(N)AT-3'/3'-T(N)A-5' base pairs are considerably stabilized than unmodified duplexes by stacking interaction of naphthalene rings in the narrow minor groove as characterized by a new emission at longer wavelength and exciton coupled CD signals. (C) 2011 Elsevier Ltd. All rights reserved.
Scientific journal, English

Synthesis of environmentally sensitive 2 '-deoxyguanosine containing solvatochromic pyrene fluorophore
Yoshio Saito; Yuta Shinohara; Shinya Ishioroshi; Azusa Suzuki; Makiko Tanaka; Isao Saito
TETRAHEDRON LETTERS, PERGAMON-ELSEVIER SCIENCE LTD, 52, 18, 2359-2361, May 2011, Peer-reviwed, The synthesis and photophysical property of novel solvatochromic pyrene derivative, Apa 5 and fluorescent guanine (APa)G 8 were described. These newly synthesized fluorescent pyrene derivatives exhibited solvent polarity dependent fluorescence at longer wavelengths. Such environmentally sensitive pyrene derivatives can be used as a reporter probe that is sensitive to the changes in the microenvironment around DNA either in vitro or in vivo. (C) 2011 Elsevier Ltd. All rights reserved.
Scientific journal, English

Design and application of novel nucleobase analogs as an environmentally sensitive probe.
Tanaka, M; Oguma, K; Kozakai, R; Kugenuma, K; Saito, Y; Saito, I
Lead, The 33th Annual Meeting of the Japanese Society for Photomedicine and Photobiology, 33, 47-48, 2011
Symposium, English

DNA-Mediated Electron Transfer in Naphthalene-Modified Oligonucleotides
Makiko Tanaka; Benjamin Elias; Jacqueline K. Barton
Lead, JOURNAL OF ORGANIC CHEMISTRY, AMER CHEMICAL SOC, 75, 8, 2423-2428, Apr. 2010, Peer-reviwed, Naphthalene-modified oligonucleotides have been synthesized and characterized with respect to electron transfer chemistry. Using the Sonogashira coupling reaction, naphthalene can be covalently anchored onto a modified uridine through an ethynyl linkage. This tethering allows for effective electronic coupling with the DNA bases, resulting in a significant red shift of the absorption bands of the naphthalenic chromophore. Modification with this chromophore does not appear to affect the overall stability and structure of the DNA. Upon selective irradiation of the naphthalene moiety at 340 nm, photoreduction of a distal electron trap, 5-bromouridine, embedded in the DNA base stack occurs. This DNA-mediated reduction from a distance was found to be significantly more efficient with substitution of 5-bromouridine toward the 5'-end than toward the 3'-end. These results support a general preference for electron transfer through DNA toward the 5'-end, irrespective of the donor. In addition, differences in efficiency of photoreduction through intrastrand and interstrand pathways are observed. For DNA-mediated reduction, as with DNA-mediated oxidation, significant differences in the charge transfer reaction are apparent that depend upon subtle differences in coupling into the DNA base stack.
Scientific journal, English

Intermolecular vs. intramolecular photoinduced electron transfer from nucleotides in DNA to acridinium ion derivatives in relation with DNA cleavage
Makiko Tanaka; Kazusa Yukimoto; Kei Ohkubo; Shunichi Fukuzumi
Lead, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, ELSEVIER SCIENCE SA, 197, 2-3, 206-212, Jun. 2008, Peer-reviwed, Photoirradiation of various 10-methylacridinium ions (AcrR(+), R = H,Pr-i, and Ph) intercalated in DNA results in ultrafast intramolecular electron transfer, followed by rapid back electron transfer between AcrR(+) and nucleotides in DNA. The electron-transfer dynamics in DNA were monitored by femtosecond time-resolved transient absorption spectroscopy. Both acridinyl radical and nucleotide radical cations, formed in the photoinduced electron transfer in DNA, were successfully detected in an aqueous solution. These transient absorption spectra were assigned by the comparison with those of DNA nucleotide radical cations, which were obtained by the intermolecular electron-transfer oxidation of nucleotides with the electron-transfer state of 9-mesityl-10-methylacridinium ion (Acr(center dot)-Mes(center dot+)) produced upon photoexcitation of Acr(+)-Mes. Photoinduced cleavage of DNA with various acridinium ions (AcrR(+), R = H, Pr-i, Ph, and Mes) has also been examined by agarose gel electrophoresis, which indicates that the rapid intramolecular back electron transfer between acridirryl radical and nucleotide radical cation in DNA suppresses the DNA cleavage as compared with the intermolecular electron-transfer oxidation of nucleotides with Acr(center dot)-Mes(center dot+). (c) 2008 Published by Elsevier B.V.
Scientific journal, English

DNA cleavage induced by photoiffadiation of coenzyme B-12 and organocobaloximes without dioxygen
Makiko Tanaka; Kei Ohkubo; Shunichi Fukuzumi
Lead, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, ELSEVIER SCIENCE SA, 197, 1, 94-100, Jun. 2008, Peer-reviwed, Homolytic cobalt-carbon bond cleavages of methylcobalamin (coenzyme B-12) and a B-12 analogue, [ (DH)(2)(CH3)Co(py)] ((DH)(2) = bis(dimethylglyoximate), py=pyridine) by UVA irradiation induced effective DNA strand scission by generated methyl radical under anaerobic conditions. The efficiency of DNA strand scission is drastically changed depending on the type of generated radicals. For example, the photoexcitation of 5'-deoxyladenosylcobalamin (one of coenzyme B-12) under anaerobic condition results in less effective DNA cleavage. No DNA cleavage has occurred under photoirradiation of (DH)(2)(PhCH2)Co(py), because the reactivity of benzyl radical formed by Co-C bond cleavage is much lower than that of methyl radical. The photoreactivity of coenzyme B-12 and organocobaloximes toward DNA nucleotides was also examined in order to compare the reactivity with those of DNA cleavage. In contrast to the efficient DNA strand scission by photoirradiation of methylcobalamin and (DH)(2)(CH3)Co(py) in the absence of oxygen, the photoirradiation in the presence of oxygen under otherwise the same experimental conditions resulted in no DNA cleavage. This indicates that the DNA cleavage by alkyl radicals is suppressed by molecular oxygen, because alky radicals are converted to the much less reactive alkylperoxyl radicals. (c) 2008 Published by Elsevier B.V.
Scientific journal, English

π-complex formation between a bisporphyrin and acridinium ion and the photodynamics
Makiko Tanaka; Kei Ohkubo; Claude P. Gros; Roger Guilard; Shunichi Fukuzumi
Lead, ECS Transactions, 2, 12, 167-176, 2007, Peer-reviwed, Acridinium ion forms a π-complex with a free-base bisporphyrin through π-π interaction in benzonitrile. Photoinduced electron transfer from a cofacial bisporphyrin to acridinium ion in a πcomplex formed between a cofacial bisporphyrin and acridinium ion results in formation of the persistent electron-transfer state at low temperature. Copyright The Electrochemical Society.
International conference proceedings, English

Persistent electron-transfer state of a pi-complex of acridinium ion inserted between porphyrin rings of cofacial bisporphyrins
Makiko Tanaka; Kei Ohkubo; Claude P. Gros; Roger Guilard; Shunichi Fukuzumi
Lead, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 128, 45, 14625-14633, Nov. 2006, Peer-reviwed, A free-base cofacial bisporphyrin, H(4)DPOx, forms a pi-complex with acridinium ion (AcH+) by pi-pi interaction in benzonitrile (PhCN). Formation of the H(4)DPOx-AcH+ pi-complex was probed by UV-vis and NMR spectroscopy. The binding constant between AcH+ and H(4)DPOx is determined as 9.7 x 10(4) M-1. Photoinduced electron transfer (ET) from the H(4)DPOx to the AcH+ moiety occurred efficiently in the d-complex to form the ET state (H(4)DPOx(center dot+)-AcH center dot). The ET state is successfully detected by laser flash photolysis. The lifetime of the ET state is 18 mu s in PhCN at 298 K, and the quantum yield of the ET state is 90%. The temperature dependence of the ET state lifetime has been examined in the range from 273 to 353 K. The ET state lifetime exhibited a large temperature dependence, and the linear plot of In(k(BET) T-1/2) vs T-1, in accordance with the Marcus equation, affords the ET reorganization energy (0.54 eV). As a result, a remarkably long-lived ET state has been attained at low temperature, and virtually no decay of the ET state was observed at 77 K. Such an extremely long-lived ET state is indeed detected by steadystate UV-vis absorption spectroscopy.
Scientific journal, English

Reductive DNA cleavage induced by UVA photoirradiation of NADH without oxygen
Makiko Tanaka; Kei Ohkubo; Shunichi Fukuzumi
Lead, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 128, 38, 12372-12373, Sep. 2006, Peer-reviwed
Scientific journal, English

DNA cleavage by UVA irradiation of NADH with dioxygen via radical chain processes
Makiko Tanaka; Kei Ohkubo; Shunichi Fukuzumi
Lead, JOURNAL OF PHYSICAL CHEMISTRY A, AMER CHEMICAL SOC, 110, 38, 11214-11218, Sep. 2006, Peer-reviwed, Efficient DNA cleaving-activity is observed by UVA irradiation of an O-2-saturated aqueous solution of NADH (beta-nicotinamide adenine dinucleotide, reduced form). No DNA cleavage has been observed without NADH under otherwise the same experimental conditions. In the presence of NADH, energy transfer from the triplet excited state of NADH ((3)NADH*) to O-2 occurs to produce singlet oxygen (O-1(2)) that is detected by the phosphorescence emission at 1270 nm. No quenching of O-1(2) by NADH was observed as indicated by no change in the intensity of phosphorescence emission of O-1(2) at 1270 nm in the presence of various concentrations of NADH. In addition to the energy transfer, photoinduced electron transfer from 3NADH* to O-2 occurs to produce NADH(center dot+) and O-2(center dot-), both of which was observed by ESR. The quantum yield of the photochemical oxidation of NADH with O-2 increases linearly with increasing concentration of NADH but decreases with increasing the light intensity absorbed by NADH. Such unusual dependence of the quantum yield on concentration of NADH and the light intensity absorbed by NADH indicates that the photochemical oxidation of NADH with O-2 proceeds via radical chain processes. The O-2(center dot-) produced in the photoinduced electron transfer is in the protonation equilibrium with HO2 center dot, which acts as a chain carrier for the radical chain oxidation of NADH with O-2 to produce NAD(+) and H2O2, leading to the DNA cleavage.
Scientific journal, English

Photodynamics in Various Complexes between Acridinium Ion and Freebase, Monozinc and Zinc Bisporphyrins.
Tanaka, M; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Lead, Journal of Porphyrins and Phthalocyanines, 10, 892-892, 2006
Symposium, English

Effects of DNA on driving force dependence of photoinduced electron transfer from the excited state of tris(2,2-bipyridine)ruthenium(II) to intercalators in DNA distinction between intra- and inter-DNA pathways
S Fukuzumi; M Tanaka; M Nishimine; K Ohkubo
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, ELSEVIER SCIENCE SA, 175, 2-3, 79-88, Oct. 2005, Peer-reviwed, Photoinduced electron transfer (ET) dynamics from the excited state of a ruthenium complex [Ru(bpy)(3)(2+) (bpy = 2,2'-bipyridine)] to a series of intercalators in DNA, 9-substituted-10-methylacridinium ions (AcrR(+), R = H, CH2Ph, Pr' and Ph), 3-substituted-1-methylquinolinium ions (RQuH(+), R = H, Me, CN and Br) and 4- and 5-methylphenanthridinium ions (4- and 5-MePhen(+)), were examined from the emission decay profiles of Ru(bpy)(3)(2+) in the absence and presence of DNA in an aqueous solution. Intercalation of AcrH(+) to DNA is found to result in inhibition of hydride transfer from an NADH model compound, 1-benzyl-1,4-dihydronicotinamide, to AcrH(+). In contrast, the rate constants of photoinduced ET of intercalated molecules to DNA become much larger than those of free intercalators in solution due to the positive shift in the one-electron reduction potentials by the intercalation into DNA. The intramolecular pathway of photoinduced ET from Ru(bpy)(3)(2+*) bound electrostatically to DNA to intercalators bound to the same DNA molecule has been distinguished from the intermolecular pathway of photoinduced ET of intercalators bound to a different DNA molecule. The occurrence of photoinduced ET is examined by laser flash photolysis experiments which show the transient absorption spectra of the one-electron reduced intercalator when the ET is exergonic. The resulting data were analyzed in light of the Marcus theory of ET to determine reorganization energies of ET in DNA as well as in an aqueous solution. (c) 2005 Elsevier B.V. All rights reserved.
Scientific journal, English

Phase transition in photo-absorbing single microemulsion droplets during the laser photophoretic migration
Hideaki Monjushiro; Makiko Tanaka; Hitoshi Watarai
Proceedings of SPIE - The International Society for Optical Engineering, 5930, 1-11, 2005, Laser-photophretic behavior of the micrometer-sized single droplets of photo-absorbing microemulsion was observed in water by irradiating a cw Ar+ ion laser. We have made an unexpected observation that the droplets repeated the expansion and the sudden contraction motion during the laser photophoretic migration. The period of the expansioncontraction cycle was decreased as the irradiated laser power was increased and was independent of the initial size of the droplet. The origin of the cyclical motion is the temperature rise of the droplet due to the photo-thermal conversion. The three-step mechanism for the periodic motion of the droplet was proposed. The first step is the phase separation of the bicontinuous microemulsion droplet into the normal w/o microemulsion outer phase and the aqueous inner phase in the droplet, which was caused by the laser-induced temperature gradient inside the droplet. In the second step, an expansion of the inner aqueous phase was induced by the percolation of the external water by thermo-osmosis, which was caused by the laser-induced temperature gradient between inside and outside of the microemulsion liquid membrane of the droplet. In the third step, the liquid membrane became thinner to a critical thickness which induced its break and the droplet shrank into the original shape. The proposed mechanism for the expansion process was confirmed by the numerical simulation of the volume change of the droplet during the laser irradiation, taking account of the heat evolution in the droplet, the heat transfer from the droplet, and the percolation of the external water by thermo-osmosis and by the high-speed CCD imaging of the contraction motion of the droplet.
International conference proceedings, English

Laser photophoretic migration with periodic expansion-contraction motion of photo-absorbing microemulsion droplets in water
M Tanaka; H Monjushiro; H Watarai
Lead, LANGMUIR, AMER CHEMICAL SOC, 20, 25, 10791-10797, Dec. 2004, Peer-reviwed, When the water-in-oil (w/o) microemulsion droplets including the Co(III)-pyridylazo complex as the photo-absorber were irradiated with a continuous-wave Ar+ ion laser (514.5 nm), we have observed unexpected phenomenon that photo-absorbing microemulsion droplets in water repeated the expansion and the sudden contraction during the laser photophoretic migration. The period of the expansion-contraction cycle was inversely proportional to both the concentration of the complex and the irradiated laser power and was independent of the initial size of the droplet. The mechanism of the periodic motion of the droplet was investigated by local temperature measurement and Raman microscope spectroscopy. It was suggested that the first step was the phase separation of the bicontinuous microemulsion droplet into the normal w/o microemulsion outer phase and the aqueous inner phase in the droplet, which was caused by the laser-induced temperature gradient inside the droplet. Subsequently, an expansion of the inner aqueous phase was induced by the percolation of the external water by thermo-osmosis, which was caused by the laser-induced temperature gradient between the inside and the outside of the microemulsion liquid membrane of the droplet. When the liquid membrane became thinner to a critical thickness, the inner aqueous phase was released and the droplet shrank into the original size. The proposed mechanism can give an account of the unique cyclical motion.
Scientific journal, English

Periodic expansion-contraction motion of photoabsorbing organic droplets during laser pbotophoretic migration in water
H Monjushiro; M Tanaka; H Watarai
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 32, 3, 254-255, Mar. 2003, Peer-reviwed, Laser photophoretic behavior of 2-benzylpyridine droplets including Co(III)-pyridylazo complex as a photoabsorber was examined in water by irradiating cw Ar+ ion laser. We have made a new observation like a "breathing" motion that the droplets repeated the expansion and the sudden contraction during laser photophoretic migration. The period of the motion decreased with increasing the Co complex concentration in the droplet and also with increasing the laser power. The origin of the motion is the water vaporization in the droplet by photothermal conversion.
Scientific journal, English

Electron transfer and guanine photodamage in DNA condensate
MAKIKO TANAKA
光化学協会, 19 Aug. 2020, PHOTOCHEMISTRY, 51, 2, 92-95, Japanese, Invited, Introduction scientific journal

Charge transfer and damage in DNA by oxidizing DNA with light
Makiko Tanaka
09 Mar. 2018, UEC e-Bulletin, 17, English, Introduction research institution

人工核酸を用いたDNA内電荷移動とDNA光損傷
田仲真紀子
日本生化学会, Aug. 2015, 生化学, 187, 4, 463-466, Japanese, Introduction scientific journal, 0037-1017, 40020571874, AN00126072

相分離生物学の全貌（白木賢太郎 編）
田仲真紀子
Scholarly book, Japanese, Contributor, 第51章 DNAの状態と電子移動, 東京化学同人, 16 Nov. 2020

2種類の短鎖DNAをビルディングブロックとした階層的な集合体の形成
牧野 哲直; 梶谷 孝; 田仲 真紀子
Oral presentation, 日本化学会 第105春季年会
26 Mar. 2025

２種類の短鎖 DNA を活用した分子集合体の階層構造の構築
牧野 哲直; 田仲 真紀子
Oral presentation, 第15回分子サイバネティクス/第59回分子ロボティクス定例研究会
06 Dec. 2024

分子混雑環境下における自己相補 DNA 鎖の集合によるファイバー状液晶の形成
白井 想; 牧野 哲直; 田仲 真紀子
Oral presentation, 第15回分子サイバネティクス/第59回分子ロボティクス定例研究会
06 Dec. 2024

2種類の短鎖DNAを用いた六角形構造に基づく階層的な自己集合
牧野 哲直; 田仲 真紀子
分子サイバネティクス第４回領域会議, Invited
29 Nov. 2024

2種類の短鎖DNAをビルディングブロックとしたDNA集合体の形態制御
牧野 哲直; 田仲真紀子
Poster presentation, 第18回バイオ関連化学シンポジウム
13 Sep. 2024

Controlling Morphology of Hexagonal Liquid Crystalline DNA
Makiko Tanaka
Poster presentation, 10th Japan-Italian Joint Workshop on Liquid Crystal (JILCW2024)
05 Sep. 2024

Hierarchically self-assembled liquid crystal built from short DNA
Makiko Tanaka
Invited oral presentation, International Congress on Pure & Applied Chemistry (ICPAC) Mongolia 2024, Invited
29 Aug. 2024

２種類の短鎖DNAによって成長方向を制御した六角形型集合体
牧野 哲直; 田仲真紀子
Poster presentation, 第35回生体機能関連化学部会若手の会サマースクール
09 Jul. 2024

Self-assembly of Short Double-stranded DNA into Micro-sized H-shaped and Hexagonal Ring Structures
Tetsunao Makino, Makiko Tanaka
Poster presentation, The 3rd International Symposium on Biofunctional Chemistry (ISBC2024)
24 Apr. 2024

二本鎖DNAのマイクロメートルサイズのH型構造体への自己集合
牧野哲直; 田仲真紀子
日本化学会第104春季年会
20 Mar. 2024

分子混雑環境下で形成する六角形DNA集合体の形成メカニズムの探索
牧野 哲直; 田仲 真紀子
第13回 CSJ化学フェスタ2023
17 Oct. 2023

Self-assembly of double-stranded DNA into hexagonal liquid crystal
Makiko Tanaka
Invited oral presentation, International Congress on Pure & Applied Chemistry (ICPAC) Bali 2023, Invited
13 Sep. 2023

Micro-sized liquid crystals composed of short double-stranded DNA
Makiko Tanaka
Invited oral presentation, The 6th Asian Chemical Biology Conference (ACBC2023), Invited
22 Aug. 2023

六角形型DNA集合体の形成におけるDNA配列と共溶質の影響
牧野哲直; 田仲真紀子
Oral presentation, 日本化学会 第103回 春季年会
23 Mar. 2023

Formation and Properties of DNA Liquid Crystalline Phase in Crowded Environment
Makiko Tanaka
Invited oral presentation, English, International Congress on Pure & Applied Chemistry (ICPAC KK 2022), Invited, International conference
25 Nov. 2022

Formation of micro-sized hexagonal DNA assemblies in aqueous-salt PEG solution
Tetsunao Makino; Daisuke Nakane; Makiko Tanaka
Poster presentation, English, The 49th International Symposium on Nucleic Acid Chemistry, International conference
02 Nov. 2022

光学顕微鏡観察可能なマイクロメートルサイズの六角形型DNA構造体の形成
牧野 哲直; 田仲 真紀子
Poster presentation, Japanese, 第１２回CSJ化学フェスタ
20 Oct. 2022

光照射によるDNA集合体の形態変化
木下 晴弥; 山口 湧暉; 田仲 真紀子
Poster presentation, Japanese, 第１２回CSJ化学フェスタ
19 Oct. 2022

Formation of double-stranded DNA assemblies and their properties under crowded conditions
牧野哲直; 山口湧暉; 田仲真紀子
Oral presentation, Japanese, 第１６回バイオ関連化学シンポジウム, Domestic conference
12 Sep. 2022

光を用いたDNA集合状態の機能探索 ～化学の視点からのDNA研究～
田仲 真紀子
Public discourse, Japanese, 奈良県⽴医科⼤学V-iCliniX講座, Invited, オンライン
28 Sep. 2021

ホールトラップ核酸塩基を用いた分子混雑環境におけるDNA内電子移動効率の評価
武富 祐樹; 山口 湧暉; 櫻井 俊亮; 田仲 真紀子
Poster presentation, Japanese, 第15回バイオ関連化学シンポジウム, オンライン, Domestic conference
09 Sep. 2021

光増感剤とホール捕捉塩基を含む液晶 DNA の形成と液晶内電子移動特性の検討
山口 湧暉; 武富 祐樹; 櫻井 俊亮; 田仲 真紀子
Poster presentation, Japanese, 第8回バイオ関連化学シンポジウム若手フォーラム, オンライン, Domestic conference
03 Sep. 2021

Electron transfer and oxidative base damage in DNA condensed phase
Makiko Tanaka
Public symposium, English, MBSJ2020 Online, Invited, Domestic conference
03 Dec. 2020

光を用いたDNAの機能と特性の探索
田仲 真紀子
2020年度UECパスポートセミナー第１回, Invited
15 Oct. 2020

Guanine damage in DNA condensate by singlet oxygen generated from photosensitizer
SAKURAI, Shunsuke; JO, Kento; KINOSHITA, Hikari; ESUMI, Mayu; TANAKA, Makiko
Oral presentation, Japanese, 第１4回バイオ関連化学シンポジウム
07 Sep. 2020

Effects of polyamines on DNA structural changes and guanine photodamage
〇Mayu Esumi; Shunsuke Sakurai; Makiko Tanaka
Oral presentation, Japanese, The 100th CSJ Annual Meeting
22 Mar. 2020

Promotion of Interduplex Electron Transfer in Ψ-type DNA
○Shunsuke Sakurai; Mayu Esumi; Makiko Tanaka
Oral presentation, Japanese, The 100th CSJ Annual Meeting
22 Mar. 2020

Promotion of guanine damage by singlet oxygen generated from SYBR Green I in DNA condensation
Jo Kento; Shunsuke Sakurai; ○Makiko Tanaka
Oral presentation, Japanese, The 100th CSJ Annual Meeting
22 Mar. 2020

DNA凝縮体中の電子移動およびグアニン光酸化損傷特性の研究
櫻井 俊亮; 江角 茉結; 城 健斗; 田仲 真紀子
Poster presentation, Japanese, 第４回LLPS研究会・ASUKA若手交流会2019, Domestic conference
09 Dec. 2019

DNAの多様な形態とその機能 –化学の視点からのDNA研究–
田仲 真紀子
スマートテクノロジーフォーラム(STF)2019 医療用テクノロジーの進歩と可能性, Invited
26 Sep. 2019

ψ型DNA中での光誘起電子移動によるグアニン酸化損傷の促進
櫻井俊亮; 江角茉結; 田仲真紀子
Poster presentation, Japanese, 第９２回日本生化学会大会
20 Sep. 2019

スペルミジンによるDNAの高次構造変化とDNA内電子移動への影響
江角茉結; 櫻井俊亮; 田仲真紀子
Poster presentation, Japanese, 第９２回日本生化学会大会
20 Sep. 2019

凝縮DNA中での電子移動によるグアニン酸化損傷の促進
櫻井 俊亮; 江角 茉結; 田仲 真紀子
Oral presentation, Japanese, 第１３回バイオ関連化学シンポジウム
04 Sep. 2019

Effect of DNA Condensation on Guanine Photooxidative Damage via Electron Transfer
Makiko Tanaka
Invited oral presentation, English, International Congress on Pure & Applied Chemistry (ICPAC) Yangon 2019, Invited, International conference
07 Aug. 2019

Effects of surrounding environments on guanine photooxidation via DNA-mediated electron transfer
○Makiko Tanaka; Shunsuke Sakurai; Mayu Esumi
Oral presentation, Japanese, 日本化学会第99回春季年会, 甲南大学 岡本キャンパス
16 Mar. 2019

Effects of Molecular Crowding on DNA-mediated Electron Transfer and Guanine Oxidation in Pyrene-modified Oligonucleotides
H. Iida; T. Matsumoto; M. Tanaka
Poster presentation, English, The Irago Conference 2018, International conference
01 Nov. 2018

Molecular crowding effect on DNA charge transfer and guanine photodamage
TANAKA, Makiko; ESUMI, Mayu; SAKUEAI, Shunsuke; IIDA, Hiroki
Oral presentation, Japanese, 第12回バイオ関連化学シンポジウム, Domestic conference
09 Sep. 2018

Effects of Molecular-crowding on Electron Transfer and Oxidative Damage in Pyrene-modified Oligonucleotides
Makiko Tanaka; Hiroki Iida; Takayuki Matsumoto
Oral presentation, Japanese, 日本化学会第98春季年会, 日本化学会, 日本大学船橋キャンパス, Domestic conference
20 Mar. 2018

Photoinduced Electron Transfer in Pyrene-modified Oligonucleotides under Crowding Conditions
Makiko Tanaka; Hiroki Iida; Takayuki Matsumoto
Poster presentation, English, The 44th International Symposium on Nucleic Acid Chemistry, International conference
Nov. 2017

光でDNAを調べる、操作する －超スマート社会における個別化医療にむけて－
田仲 真紀子
創立100周年記念公開講座 －超スマート社会の実現を目指す最先端の科学・技術研究 第３回, Invited
05 Aug. 2017

Ribonuclease A as Effective Promoter for Unimolecular Invasion of Peptide Nucleic Acid to Double-stranded DNA
TANAKA, Makiko; SHIGI, Narumi; KOMIYAMA, Makoto
Oral presentation, Japanese, The 96th CSJ Annual Meeting, Domestic conference
25 Mar. 2016

Promotion of peptide nucleic acid invasion into double-stranded DNA by RNase A
田仲真紀子; 鴫成実; 小宮山眞
Poster presentation, Japanese, BMB2015(第３８回 日本分子生物学会年会、第８８回日本生化学大会 合同大会)
03 Dec. 2015

チアゾールオレンジ修飾ペプチド核酸による二本鎖DNA中での特定配列の蛍光検出と位置選択的光損傷
田仲真紀子; 鴫 成実; 須磨岡 淳; 小宮山 眞
Oral presentation, Japanese, 日本化学会第95春季年会, Domestic conference
Mar. 2015

Efficient excess electron transfer through modified DNA using 2’-deoxyuridine derivatives
Makiko Tanaka; Kazuhiro Oguma; Yoshio Saito; Isao Saito
Poster presentation, English, The 39th International Symposium on Nucleic Acid Chemistry, International conference
Nov. 2012

フェニルエチニル基を有するデオキシウリジン誘導体を電子メディエーターとする修飾DNA内の高効率過剰電子移動
田仲真紀子; 小熊一裕; 齋藤義雄; 齋藤烈
Poster presentation, Japanese, 2012年光化学討論会, 東工大, Domestic conference
Sep. 2012

DNA二本鎖に複数導入した修飾核酸塩基の光物性
田仲真紀子; 小熊一裕; 齋藤義雄; 齋藤烈
Oral presentation, Japanese, 日本化学会第92春季年会, 日本大学, Domestic conference
Mar. 2012

Synthesis and properties of oligodeoxynucleotides containing 2-substituted adenine
Tanaka, M; Kozakai, R; Oguma, K; Saito, Y; Saito, I
Poster presentation, English, The 38th International Symposium on Nucleic Acid Chemistry, Sapporo, Japan, International conference
Nov. 2011

環境感応型蛍光プローブとなる新規核酸塩基誘導体の開発とその応用
田仲真紀子; 小熊一裕; 小坂井亮太; 久下沼賢志; 齋藤義雄; 齋藤烈
Oral presentation, Japanese, 第33回光医学光生物学会, 大阪大学, Domestic conference
Jul. 2011

芳香族化合物をエチニルリンカーで連結した核酸塩基誘導体の光物性
田仲真紀子; 小熊一裕; 小坂井亮太; 齋藤義雄; 齋藤烈
Oral presentation, Japanese, 日本化学会第91春季年会, 神奈川大学, Domestic conference
Mar. 2011

新規な発色団修飾塩基を光誘起電子供与体として含むDNA内の過剰電子移動特性
田仲真紀子; Benjamin Elias; Jacqueline K. Barton
Oral presentation, Japanese, 2009年光化学討論会, 群馬県桐生市, Domestic conference
Sep. 2009

DNA中の核酸塩基とアクリジニウムイオンの分子間および分子内光誘起電子移動
田仲真紀子; 行本和紗; 大久保敬; 福住俊一
Poster presentation, Japanese, ２００７年光化学討論会, Domestic conference
Sep. 2007

補酵素類縁体を用いたDNA光損傷とその光ダイナミクス
田仲真紀子; 大久保 敬; 福住俊一
Oral presentation, Japanese, 日本化学会第86春季年会, 関西大, Domestic conference
Mar. 2007

Photoreductive DNA Cleavage Induced by UVA Irradiation of Coenzyme
Tanaka, M; Ohkubo, K; Fukuzumi, S
Oral presentation, English, The International Symposium on Advanced Science and Biotechnology 2007, Osaka, International conference
Mar. 2007

酵素およびそのモデル化合物の光励起によって発生させた活性ラジカル種によるDNA切断
田仲真紀子; 大久保 敬; 福住俊一
Oral presentation, Japanese, 生体機能関連化学シンポジウム, 京都大学, Domestic conference
Sep. 2006

NADHによるDNAの光電子移動還元と切断機構
田仲真紀子; 大久保 敬; 福住俊一
Poster presentation, Japanese, 2006年光化学討論会, 東北大, Domestic conference
Sep. 2006

π−Complex Formation between Various Bisporphyrins and Acceptor ion and the Photodynamics
Tanaka, M; Amasaki, I; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Oral presentation, English, The 209th Meeting of the Electrochemical Society, Denver, Colorado, International conference
May 2006

Generation of Triplet Excited State of NADH by UVA Irradiation and DNA Cleavage Mechanism
Tanaka, M; Ohkubo, K; Fukuzumi, S
Poster presentation, English, XXIst IUPAC Symposium on Photochemistry, Kyoto, International conference
Apr. 2006

NADHのUVA照射による活性酸素種発生機構とDNA損傷
田仲真紀子; 大久保 敬; 福住俊一
Oral presentation, Japanese, 日本化学会第86春季年会, 日本大学, Domestic conference
Mar. 2006

Formation of Supramolecular Complex between Porphyrin and NAD+ Analogs by π−π Interaction and Photoinduced Electron Transfer
Tanaka, M; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Poster presentation, English, The 2005 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2005), Hawaii, USA, International conference
Dec. 2005

Extremely Long-Lived Charge Separated State in π-Complex of Bisporphyrins with Acridinium Ion
Tanaka, M; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Oral presentation, English, 2005 Korea-Japan Symposium on Frontier Photoscience, International conference
Oct. 2005

NADHおよびそのモデル化合物によるDNA光損傷
田仲真紀子; 大久保 敬; 福住俊一
Oral presentation, Japanese, 2005年光化学討論会, 福岡, Domestic conference
Sep. 2005

NADHによる活性酸素種発生と光DNA切断
田仲真紀子; 大久保 敬; 福住俊一
Poster presentation, Japanese, 第20回生体機能関連化学シンポジウム, 名古屋市大, Domestic conference
Sep. 2005

Phase transition in photo-absorbing single microemulsion droplets during the laser photophoretic migration
Hideaki Monjushiro; Makiko Tanaka; Hitoshi Watarai
Oral presentation, English, SPIE 5930, Optical Trapping and Optical Micromanipulation II, San Diego, California, USA, International conference
26 Aug. 2005

Photodynamics of Supramolecular Complexes of Bisporphyrins with NAD+ Analogs by π−π Interaction
Tanaka, M; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Oral presentation, English, The 207th Meeting of the Electrochemical Society, Quebec City, Canada, International conference
May 2005

ポルフィリン多量体とNAD+類縁体のπ−π相互作用による超分子錯体形成と光電子移動
田仲真紀子; 大久保 敬; Claude P. Gros、Roger Guilard; 福住俊一
Oral presentation, Japanese, 日本化学会第８５春季年会, 神奈川大学, Domestic conference
Mar. 2005

π-Complex Formation and Photoinduced Electron Transfer between Acridinium Ion and a Cofacial Bisporphyrin
Tanaka, M; Ohkubo, K; Gros, C. P; Guilard, R; Fukuzumi, S
Poster presentation, English, The 5th International 21 Century COE Symposium on Integrated EcoChemistry, Osaka, International conference
Jan. 2005

フリーベースビスポルフィリン-アクリジニウムイオン超分子錯体のフォトダイナミクス
田仲真紀子; 大久保 敬; C; P. Gros; R. Guilard; 福住俊一
Oral presentation, Japanese, ２００４年光化学討論会, つくば国際会議場, Domestic conference
Nov. 2004

DNA中におけるトリスビピリジンルテニウム(II)錯体からNAD+類縁体への光誘起電子移動反応のドライビングフォース依存性
田仲真紀子; 西峯麻里; 大久保 敬; 福住俊一
Poster presentation, Japanese, 2004年光化学討論会, つくば国際会議場, Domestic conference
Nov. 2004

NAD+ 類縁体の電子移動還元反応におけるスタッキング効果
田仲真紀子; 西峯麻里; 大久保 敬; 福住俊一
Poster presentation, Japanese, 第19回生体機能関連シンポジウム, 東京大学, Domestic conference
Oct. 2004

Photoinduced Electron Transfer in Free-base Bisporphyrin-Acridinium Ion Supramolecular Complexes
Tanaka, M; Ohkubo, K; Gros, C. P; Burdet, F; Barbe, J.-M; Guilard, R; Fukuzumi, S
Poster presentation, English, CREST International Symposium on Radical Ion Reactivity, Hyogo, International conference
Jun. 2004

Co(III)-par錯体を抽出した微小液滴のレーザー光泳動の解析
田仲 真紀子; 文珠四郎; 秀昭; 渡會 仁
Oral presentation, Japanese, 日本化学会第84春季年会, 関西学院大学(西宮市), Domestic conference
Mar. 2004

Co(III)-par 錯体を抽出した微小液滴のレーザー光泳動の解析
田仲 真紀子; 文珠四郎; 秀昭; 渡會 仁
Poster presentation, Japanese, 特定領域研究(B)「液液界面ナノ領域の化学」 第5回公開シンポジウム, グランキューブ大阪(大阪国際会議場)(大阪市), Domestic conference
Dec. 2003

Co(III)-par錯体を抽出した微小液滴のレーザー光泳動の解析
田仲 真紀子; 文殊四郎; 秀昭; 渡會 仁
Oral presentation, Japanese, 日本分析化学会第52年会, 宮城教育大学(仙台市), Domestic conference
Sep. 2003

Periodic Expansion and Contraction of Photo-Absorbing Organic Droplets during Laser Photophoresis in Water
Hideaki Monjushiro; Makiko Tanaka; Hitoshi Watarai
Poster presentation, English, XXIst International Conference on Photochemistry, Nara, International conference
Jul. 2003

O/Wエマルション液滴のレーザー光泳動における周期的膨張現象
田仲 真紀子; 文珠四郎; 秀昭; 渡會 仁
Oral presentation, Japanese, 日本分析化学会第51年会, 北海道大学(札幌市), Domestic conference
Sep. 2002

システム生物学
Present
The University of Electro-Communications

化学概論第一
Present
The University of Electro-Communications

生命分子化学特論
Present
The University of Electro-Communications

現代化学
Present
The University of Electro-Communications

化学生命工学実験第二
Present
The University of Electro-Communications

システム生物学
電気通信大学

化学概論第一
電気通信大学

生命分子化学特論
電気通信大学

機器分析学
The University of Electro-Communications

機器分析学
電気通信大学

現代化学
電気通信大学

化学生命工学実験第二
電気通信大学

生体機能システム実験第二
The University of Electro-Communications

現代化学 第４回
The University of Electro-Communications

現代化学 第４回
The University of Electro-Communications

現代化学 第４回
電気通信大学

生体機能システム実験第二
The University of Electro-Communications

生体機能システム実験第二
電気通信大学

高分子学会

日本化学会

光化学協会

日本生化学会

DNAの階層的自己集合による超分子ポリマーの構築と制御
田仲 真紀子
日本学術振興会, 科学研究費助成事業, 電気通信大学, 基盤研究(C), 25K08740
01 Apr. 2025 - 31 Mar. 2028

短鎖DNAの集合制御による高効率電子シグナル伝達システムの創出
田仲 真紀子
日本学術振興会, 科学研究費助成事業 学術変革領域研究(A), 電気通信大学, 学術変革領域研究(A), Principal investigator, 23H04409
Apr. 2023 - Mar. 2025

光を用いたDNAメゾスコピック凝縮反応場の機能分析
田仲真紀子(庄司真紀子)
日本学術振興会, 科学研究費助成事業 基盤研究(C), 電気通信大学, 基盤研究(C), Principal investigator, 本研究では、種々の分子夾雑・混雑環境でのDNA凝縮体の形成条件を検討し、メゾスコピックスケールの可逆的な微小凝縮場の形成がDNAに与える機能の解明を目指した。具体的には相分離凝縮場でのDNAの電子移動と核酸塩基損傷特性に着目し、光を用いて化学的アプローチにより解析を行うことで生体分子の微小反応場としての凝縮体の性質を調べた。DNA凝縮場でグアニン塩基、またその連続配列が電子移動により酸化損傷を受ける効率およびその特性については、まだほとんどが未解明である。そこで当該年度の研究ではまずホール捕捉により開環反応を起こすグアニン類縁体を含むDNA鎖を固相合成により合成し、光増感剤を含むDNA鎖とアニーリングにより相補鎖とした。この修飾DNAについてポリエチレングリコールを用いて人工的に作製した分子混雑環境下で液晶とした。修飾DNAを含む液晶について、光照射による電子移動をスタートさせグアニン酸化損傷を誘発する手法により、電子移動特性を評価した。高速液体クロマトグラフィーによる損傷塩基の定量を行い、それぞれの凝縮場での電子移動効率の評価を行った。その結果、凝縮場における電子移動効率の大幅な増大が観測された。解析の結果、液晶中でスタッキングした異なるDNA鎖間での電子移動が起こったことが示唆された。
当初は購入を予定していた備品の凍結乾燥機については消耗品代その他の研究費用がかさみ、購入できなかったため、デモ機の使用とあわせ、時間とサンプルおよび消耗品であるカラム代のロスがあるが、遠心濃縮とカラムを用いてDNAの精製をおこなった。その他、実験に使用する小型機器、試薬、合成DNA、HPLCカラム、チップやチューブ等を購入した。, 21K05108
Apr. 2021 - Mar. 2024

グアニン損傷を誘発するDNA電荷移動シグナルの生体内条件での経路探索
田仲真紀子
戸部眞紀財団, 研究助成, Principal investigator
Sep. 2018 - Aug. 2019

Creation of new genome science using chemical tools to precisely detect modification and damage of human genome
Komiyama Makoto; SHIGI Narumi; Tanaka Makiko; Rajendran Arivazhagan
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), National Institute for Materials Science, Grant-in-Aid for Scientific Research (A), Coinvestigator not use grants, We already developed super artificial restriction enzyme. In this research, we added affinity separating ability to this tool, and constructed the second- generation super artificial restriction enzyme. By modifying biotin to PNA which is a constituent of super restriction enzyme, we successfully extracted desired DNA fragments of several thousand base pairs in high purity and high yield from the human genome with 3 billion base pairs. Furthermore, by combining this biotin-modified PNA with S1 nuclease, we also succeeded in extracting desired DNA fragments from the human genome in one pot., 15H02189
Apr. 2015 - Mar. 2018

高効率DNA内シグナル伝達システムの構築とDNA光切断を用いた遺伝子操作法の開発
田仲真紀子(庄司真紀子)
日本学術振興会・特別研究員奨励費RPD, Principal investigator
2013 - 2015

Design of Environmentally Sensitive Fluorescent Nucleosides and Their Application to Innovative Fluorescence Probe for Gene Analysis
SAITO Isao; SAITO Yoshio
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Nihon University, Grant-in-Aid for Scientific Research (B), Coinvestigator, We have designed a novel type of base-discriminating fluorescent (BDF) nucleosides which are used for SNP genotyping and gene detection. We also succeeded to design environmentally sensitive fluorescent (ESF) nucleosides that emit strong fluorescence at different wavelengths depending upon a difference of local environment near the ESF base. The newly designed ESF nucleosides may discriminate structural changes such as single strands, matches, mismatches and deletions when incorporated in DNA by a significant change of emission wavelengths as well as their intensities. Quencher and end free molecular beacons (MB) were discovered for the first time by using newly designed ESF nucleosides. These low cost MB were used for the gene detection in a cell. ESF nucleosides that are sensitive to pH were also designed. Such pH sensor ESF nucleoside that emits strong fluorescence only at bellow pH 5.5 was used for the detection of cancer cell., 23350082
Apr. 2011 - Mar. 2014

新規π拡張核酸塩基の開発によるDNA内過剰電子移動特性の解明とその応用展開
田仲真紀子(庄司真紀子)
公益信託山村富美記念女性自然科学者研究助成基金, Principal investigator
2011 - 2012

光誘起電子移動による各種DNA損傷の検討と新規DNA光操作法の開発
田仲真紀子
日本学術振興会・特別研究員奨励費PD, Principal investigator
2007 - 2009

メゾスコピック生体系レーザー光誘起電子移動過程の解析と応用
田仲真紀子
日本学術振興会・特別研究員奨励費DC2, Principal investigator
2005 - 2006

プレスリリース「２種類の二本鎖DNAが階層的に自己集合することを発見」
Myself, 電気通信大学、東京科学大学, Others
Jan. 2025

ChemBioChem（Wiley-VCH）採択論文の研究をニュースリリース
電気通信大学, Others
Nov. 2022

ChemBioChem（Wiley-VCH）の採択論文内容がChemistryViewsのニュースとしてハイライト配信
Chemistry Europe, Others
Oct. 2022

第15回分子サイバネティクス/第59回分子ロボティクス定例研究会
Academic society etc, Others, 06 Dec. 2024

調査・研究会などの実績 文部科学省 科学技術予測センター専門調査員
Academic society etc, Academic research planning, 01 Apr. 2020 - 31 Mar. 2022

第４回LLPS研究会・ASUKA若手交流会2019
Academic society etc, Others, 09 Dec. 2019

カリフォルニア工科大学（日本学術振興会特別研究員 海外渡航制度）
Others, Others, Sep. 2007 - Mar. 2009