Masumi TAKI
| Department of Engineering Science | Professor |
| Cluster III (Fundamental Science and Engineering) | Professor |
| Institute for Advanced Science | Professor |
Researcher Information
Research Keyword
Career
- Sep. 2025 - Mar. 2026
Nihon University, College of Humanities and Sciences, 非常勤講師 - Sep. 2023 - Mar. 2024
日本大学 文理学部 非常勤講師 - Sep. 2021 - Mar. 2022
日本大学, 文理学部, 非常勤講師 - 01 Apr. 2021
電気通信大学大学院・情報理工学研究科, 教授 - 01 Dec. 2011 - 31 Mar. 2021
電気通信大学大学院・情報理工学研究科, 准教授 - 2017 - 2017
ETH Zürich, Department of Health Sciences and Technology, Visiting Professor - 01 Dec. 2011
電気通信大学大学院・情報理工学研究科, 准教授 - 01 Aug. 2003 - 30 Nov. 2011
岡山大学工学部, 助手(研究指導担当)、（のちに大学院工学研究科助教） - 01 Aug. 2007 - 31 Mar. 2008
米国カリフォルニア工科大学（Caltech）生物科, 客員研究員 - 01 Apr. 2003 - 31 Jul. 2003
産総研・つくば研究所, ジーンファンクション研究ラボ - 01 May 2002 - 31 Mar. 2003
(株)ジェノファンクション, 研究部・研究員 - 01 Apr. 2002 - 30 Apr. 2002
産総研・つくば研究所, ジーンファンクション研究ラボ - 01 Apr. 2000 - 31 Mar. 2002
東京大学工学部, 日本学術振興会特別研究員（ＰＤ） - 01 Apr. 1998 - 31 Mar. 2000
岡山大学工学部, 日本学術振興会特別研究員（ＰＤ） - 01 Apr. 1996 - 31 Mar. 1998
群馬大学工学部, 日本学術振興会特別研究員（DC1)
Research Activity Information
Award
Paper
- ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead
Masumi Taki; Masayasu Kuwahara; Chaohui Li; Naoko Tomoda; Naoyuki Miyashita; Tetsuo Kan; Jay Yang
Lead, Chemical Communications, Royal Society of Chemistry (RSC), 60, 100, 14964-14967, Dec. 2024, Peer-reviwed, A covalent binder was identified using a single-round screening from a latent-warhead-modified DNA library, coupled with top k-mer analysis to pinpoint the optimal warhead position on the aptamer.
Scientific journal, English - bioTCIs: Middle-to-Macro Biomolecular Targeted Covalent Inhibitors Possessing Both Semi-Permanent Drug Action and Stringent Target Specificity as Potential Antibody Replacements
Jay Yang; Yudai Tabuchi; Riku Katsuki; Masumi Taki
Last, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24, 4, Feb. 2023, Peer-reviwed, Invited
Scientific journal, English - Relative Nuclease Resistance of a DNA Aptamer Covalently Conjugated to a Target Protein
Y. Tabuchi; J. Yang; M. Taki
Last, International Journal of Molecular Sciences, 23, 7778, 14 Jul. 2022, Peer-reviwed, Invited
Scientific journal, English - Solvatochromic peptidic binder obtained via extended phage display acts as a fluororeporter for fragment-based drug discovery (FBDD)
Riku Katsuki; Tsubasa Numayama; Yudai Tabuchi; Jaiyam Sharma; Naohito Satake; Adarsh Sandhu; Masumi Taki
Last, Anal. Bioanal. Chem., Springer Nature, 414, 4803-4807, 04 Jun. 2022, Peer-reviwed
Scientific journal, English - Covalent Biologics：中・高分子型共有結合性薬剤
田淵 雄大; 瀧 真清
Last, ファルマシア, 日本薬学会, 57, 11, 1019-1024, Nov. 2021, Peer-reviwed, Invited
Scientific journal, Japanese - 薬剤の中和が可能なアプタマー型共有結合性薬剤の開発
田淵 雄大; 瀧 真清
Last, 生物工学会誌, 日本生物工学会, 99, 4, 172-175, 22 Apr. 2021, Invited
Scientific journal, Japanese - Inhibition of thrombin activity by a covalent-binding aptamer and reversal by the complementary strand antidote
Y. Tabuchi; J. Yang; M. Taki
Last, Chem. Commun., RSC, 57, 20, 2483-2486, 11 Mar. 2021, Peer-reviwed, True, Alleviating the potential risk of irreversible adverse drug effects has been an important and challenging issue for the development of covalent drugs. Here we created a DNA-aptamer-type covalent drug by introducing a sulfonyl fluoride warhead at appropriate positions of the thrombin binding aptamer to create weaponized covalent drugs. We showed the de-activation of thrombin by the novel modality, followed by its re-activation by the complementary strand antidote at an arbitrary time. We envision that such on-demand reversal of covalent drugs will alleviate the major concern of potentially irreversible ADEs and accelerate the translational application of covalent aptamer drugs.
Scientific journal, English - Medium-firm drug-candidate library of cryptand-like structures on T7 phage: Design and selection of strong binder for Hsp90
K. Mochizuki; L. Matsukura; Y. Ito; N. Miyashita; M. Taki
Last, Org. Biomol. Chem, 19, 1, 146-150, 07 Jan. 2021, Peer-reviwed, True, We designed and synthesized a medium-firm drug-candidate library of cryptand-like structures possessing a randomized peptide linker on the bacteriophage T7. From the macrocyclic library with a 109 diversity, we obtained a binder toward a cancer-related protein (Hsp90) with an antibody-like strong affinity (KD = 62 nM) and the binding was driven by the enthalpy. The selected supramolecular ligand inhibited Hsp90 activity by site-specific binding outside of the well-known ATP-binding pocket on the N-terminal domain (NTD).
Scientific journal, English - Direct screening of a target-specific covalent binder: stringent regulation of warhead reactivity in a matchmaking environment
Yudai Tabuchi; Takahito Watanabe; Riku Katsuki; Yuji Ito; Masumi Taki
Last, Chemical Communications, Royal Society of Chemistry (RSC), 57, 44, 5378-5381, 2021, Peer-reviwed,To find targeted covalent biologics, we demonstrated a direct screening method of a peptidic covalent binder
via reactivity/affinity-based co-selection using T7 phage display.
Scientific journal - Strategic design to create HER2-targeting proteins with target-binding peptides immobilized on a fibronectin type III domain scaffold
Wanaporn Yimchuen; Tetsuya Kadonosono; Yumi Ota; Shinichi Sato; Maika Kitazawa; Tadashi Shiozawa; Takahiro Kuchimaru; Masumi Taki; Yuji Ito; Hiroyuki Nakamura; Shinae Kizaka-Kondoh
RSC Advances, 10, 26, 15154-15162, Apr. 2020, Peer-reviwed
Scientific journal, English - Facile and Efficient Chemoenzymatic Semisynthesis of Fc-Fusion Compounds for Half-Life Extension of Pharmaceutical Components.
Shigeo Hirasawa; Yoshiro Kitahara; Yoriko Okamatsu; Tomohiro Fujii; Akira Nakayama; Satoko Ueno; Chiori Ijichi; Fumie Futaki; Kunio Nakata; Masumi Taki
Last, Bioconjugate chemistry, ACS, 30, 9, 2323-2331, 18 Sep. 2019, Peer-reviwed, True, The formation of Fc-fusions, in which biologically active molecules and the Fc fragment of antibodies are linked to each other, is one of the most efficient and successful half-life extension technologies to be developed and applied to peptide and protein pharmaceuticals thus far. Fc-fusion compounds are generally produced by recombinant methods. However, these cannot be applied to artificial middle molecules, such as peptides with non-natural amino acids, unnatural cyclic peptides, or pharmaceutical oligonucleotides. Here, we developed a simple, efficient, semisynthetic method for Fc-fusion production involving our previously developed enzymatic N-terminal extension reaction (i.e., NEXT-A reaction) and strain-promoted azide-alkyne cycloaddition, achieving quantitative conversion and high selectivity for the N-terminus of the Fc protein. An Fc-fusion compound prepared by this method showed comparable biological activity to that of the original peptide and a long-circulating plasma half-life. Thus, the proposed method is potentially applicable for the conjugation of a wide range of pharmaceutical components.
Scientific journal, English - Mechano-chromic protein–polymer hybrid hydrogel to visualize mechanical strain
M. Taki; T. Yamashita; K. Yatabe; V. Vogel
Lead, Soft Matter, The Royal Society of Chemistry, 15, 46, 9388-9393, Sep. 2019, Peer-reviwed,A mechano-chromic hydrogel was synthesized here via chemoenzymatic click conjugation of fluorophore-labeled fibronectin into a synthetic hydrogel copolymers. The optical FRET response could be tuned by macroscopic stretching.
Scientific journal, English - Cysteine-reactive small photo-crosslinker possessing caged-fluorescence property: binding-site determination of a combinatorially-selected peptide by fluorescence imaging / tandem mass spectrometry
K. Yatabe; M. Hisada; Y. Tabuchi; M. Taki
Last, International Journal of Molecular Sciences, MDPI AG, 19, 11, 3682-3682, 21 Nov. 2018, Peer-reviwed, Invited, To determine the binding-site of a combinatorially-selected peptide possessing a fluoroprobe, a novel cysteine reactive small photo-crosslinker that can be excited by a conventional long-wavelength ultraviolet handlamp (365 nm) was synthesized via Suzuki coupling with three steps. The crosslinker is rationally designed, not only as a bioisostere of the fluoroprobe, but as a caged-fluorophore, and the photo-crosslinked target protein became fluorescent with a large Stokes-shift. By introducing the crosslinker to a designated sulfhydryl (SH) group of a combinatorially-selected peptide, the protein-binding site of the targeted peptide was deduced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)/fluorescence imaging followed by matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry (MALDI-TOF-MS/MS) analysis.
Scientific journal, English - Fluorescent "keep-on" type pharmacophore obtained from dynamic combinatorial library of Schiff bases
Y. Tabuchi; M. Taki
Last, Anal. Bioanal. Chem., Springer Nature, 410, 26, 6713-6717, 11 Aug. 2018, Peer-reviwed
Scientific journal, English - Combinatorially Screened Peptide as Targeted Covalent Binder: Alteration of Bait-Conjugated Peptide to Reactive Modifier
Shuta Uematsu; Yudai Tabuchi; Yuji Ito; Masumi Taki
Last, Bioconjugate Chemistry, American Chemical Society, 29, 6, 1866-1871, 20 Jun. 2018, Peer-reviwed
Scientific journal, English - Combinatorially screened peptide as targeted covalent binder
S. Uematsu; Y. Tabuchi; Y. Ito; M. Taki
Last, Proc. 35th Eur. Pept. Symp., 24, 272-S153, 2018
International conference proceedings, English - Selection of Turning-on Fluorogenic Probe as Protein-Specific Detector Obtained via the 10BASE(d)-T
Shuta Uematsu; Taiki Midorikawa; Yuji Ito; Masumi Taki
Last, IRAGO CONFERENCE 2016: 360 DEGREE OUTLOOK ON CRITICAL SCIENTIFIC AND TECHNOLOGICAL CHALLENGES FOR A SUSTAINABLE SOCIETY, 1807, 020028, 2017, Peer-reviwed
International conference proceedings, English - F-18-Containing Positron Emission Tomography Probe Conjugation Methodology for Biologics as Specific Binders for Tumors
Kenji Arimitsu; Hiroyuki Kimura; Yoshinari Arai; Kazuto Mochizuki; Masumi Taki
Last, CURRENT TOPICS IN MEDICINAL CHEMISTRY, 16, 24, 2703-2724, 2016, Peer-reviwed, Invited
Scientific journal, English - Chemical and Biological Technology for In Vivo and Molecular Imaging
Masumi Taki
Lead, CURRENT TOPICS IN MEDICINAL CHEMISTRY, 16, 24, 2635-2637, 2016, Invited
Scientific journal, English - Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T
Masumi Taki; Hiroaki Inoue; Kazuto Mochizuki; Jay Yang; Yuji Ito
Last, ANALYTICAL CHEMISTRY, 88, 2, 1096-1099, Jan. 2016, Peer-reviwed
Scientific journal, English - Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase
Masumi Taki; Hiroyuki Kuroiwa
Lead, AMINO ACIDS, 47, 6, 1279-1282, Jun. 2015, Peer-reviwed
Scientific journal, English - Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASE(d)-T on Bacteriophage T7
Yuuki Tokunaga; Yuuki Azetsu; Keisuke Fukunaga; Takaaki Hatanaka; Yuji Ito; Masumi Taki
Last, MOLECULES, 19, 2, 2481-2496, Feb. 2014, Peer-reviwed, Invited
Scientific journal, English - Construction of a crown ether-like supramolecular library by conjugation of genetically-encoded peptide linkers displayed on bacteriophage T7
Keisuke Fukunaga; Takaaki Hatanaka; Yuji Ito; Michiko Minami; Masumi Taki
Last, CHEMICAL COMMUNICATIONS, 50, 30, 3921-3923, 2014, Peer-reviwed
Scientific journal, English - Gp10 based-thioetherification (10BASEd-T) on a displaying library peptide of bacteriophage T7
Keisuke Fukunaga; Takaaki Hatanaka; Yuji Ito; Masumi Taki
Last, Molecular BioSystems, 9, 12, 2988-2991, Dec. 2013, Peer-reviwed
Scientific journal, English - Kinetic analysis of the leucyl/phenylalanyl-tRNA-protein transferase with acceptor peptides possessing different N-terminal penultimate residues
Jun Kawaguchi; Kumino Maejima; Hiroyuki Kuroiwa; Masumi Taki
Last, FEBS OPEN BIO, 3, 252-255, 2013, Peer-reviwed
Scientific journal, English - Practical tips for construction of custom peptide libraries and affinity selection by using commercially available phage display cloning systems
Keisuke Fukunaga; Masumi Taki
Last, Journal of Nucleic Acids, 2012, Article ID 295719, doi:10.1155, 2012, Peer-reviwed, Invited
Scientific journal, English - Synthesis of a cyclic peptide/protein using the NEXT-A reaction followed by cyclization
Toshimasa Hamamoto; Masahiko Sisido; Takashi Ohtsuki; Masumi Taki
CHEMICAL COMMUNICATIONS, 47, 32, 9116-9118, 2011, Peer-reviwed
Scientific journal, English - Fission yeast Ubrl ubiquitin ligase influences the oxidative stress response via degradation of active Papl bZIP transcription factor in the nucleus
K.Kitamura; M.Taki; N.Tanaka; I.Yamashita
Mol Microbiol, 80, 839, 2011, Peer-reviwed
Scientific journal, English - Introduction of a Highly Photodurable and Common-laser Excitable Fluorescent Amino Acid into a Peptide as a FRET Acceptor for Protease Cleavage Detection
Masumi Taki; Yoshito Yamazaki; Yuto Suzuki; Masahiko Sisido
CHEMISTRY LETTERS, 39, 8, 818-819, Aug. 2010, Peer-reviwed
Scientific journal, English - N-Terminal Specific Point-Immobilization of Active Proteins by the One-Pot NEXT-A Method
Keitaro Ebisu; Hiroaki Tateno; Hiroyuki Kuroiwa; Koshi Kawakami; Megumi Ikeuchi; Jun Hirabayashi; Masahiko Sisido; Masumi Taki
CHEMBIOCHEM, 10, 15, 2460-2464, Oct. 2009, Peer-reviwed
Scientific journal, English - The NEXT-A (N-terminal extension with transferase and ARS) reaction
M. Taki; H. Kuroiwa; M. Sisido
Nucleic Acids Symp. Ser., 53, 1, 37-38, 2009
International conference proceedings, English - Chemoenzymatic transfer of fluorescent non-natural amino acids to the N terminus of a protein/peptide
Masumi Taki; Hiroyuki Kuroiwa; Masahiko Sisido
CHEMBIOCHEM, 9, 5, 719-722, Mar. 2008, Peer-reviwed
Scientific journal, English - Leucyl/phenylalanyl(L/F)-tRNA-protein transferase-mediated aminoacyl transfer of a nonnatural amino acid to the N-terminus of peptides and proteins and subsequent functionalization by bioorthogonal reactions
Masumi Taki; Masahiko Sisido
BIOPOLYMERS, 88, 2, 263-271, 2007, Peer-reviwed
Scientific journal, English - Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system
Masumi Taki; Yasunori Tokuda; Takashi Ohtsuki; Masahiko Sisido
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102, 6, 511-517, Dec. 2006, Peer-reviwed
Scientific journal, English - Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal arg/lys units in posttranslationally processed proteins with non-natural amino acids
Masumi Taki; Atsushi Kuno; Shinsuke Matoba; Yuki Kobayashi; Junichiro Futami; Hiroshi Murakami; Hiroaki Suga; Kazunari Taira; Tsunemi Hasegawa; Masahiko Sisido
CHEMBIOCHEM, 7, 11, 1676-+, Nov. 2006, Peer-reviwed
Scientific journal, English - Expanding the genetic code in a mammalian cell line by the introduction of four-base codon/anticodon pairs
M Taki; J Matsushita; M Sisido
CHEMBIOCHEM, 7, 3, 425-428, Mar. 2006, Peer-reviwed
Scientific journal, English - Efficient Incorporation of a Nonnatural Amino Acid into a Protein in an Insect Cell-free Translation System
Y. Tokuda; M. Taki; M. Sisido
Nucleic Acids Symp. Ser., 48, 161-162, 2006
International conference proceedings, English - Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing
H Hamada; N Kameshima; A Szymanska; K Wegner; L Lankiewicz; H Shinohara; M Taki; M Sisido
BIOORGANIC & MEDICINAL CHEMISTRY, 13, 10, 3379-3384, May 2005, Peer-reviwed
Scientific journal, English - Creation of artificial fluorescence protein in which a novel fluorescent nonnatural amino acid is site-specifically incorporated for new biosensing
H. Hamada; H. Shinohara; N. Kameshima; M. Taki; A. Syzmanska; T. Hohsaka; M. Sisido
Chemical Sensors, 21(Suppl. A), 184-186, 2005
International conference proceedings, English - Synthesis of novel luminescent substrates and their incorporation into a protein only at a terminal site via a transglutaminase-catalyzed enzymatic reaction
M Taki; K Taira
CHEMISTRY LETTERS, 33, 3, 234-235, Mar. 2004, Peer-reviwed
Scientific journal, English - Transglutaminase-mediated N- and C-terminal fluorescein labeling of a protein can support the native activity of the modified protein
M Taki; M Shiota; K Taira
PROTEIN ENGINEERING DESIGN & SELECTION, 17, 2, 119-126, Feb. 2004, Peer-reviwed
Scientific journal, English - Small-interfering-RNA expression in cells based on an efficiently constructed Dumbbell-shaped DNA
M Taki; Y Kato; M Miyagishi; Y Takagi; K Taira
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 43, 24, 3160-3163, 2004, Peer-reviwed
Scientific journal, English - Enzymatic N- and C- terminal fluorescein labelling of a protein in vitro can support the native activity of the modified protein
M. Taki; M. Shiota; K. Taira
Protein Eng, 17, 119-126, 2004, Peer-reviwed
Scientific journal, English - Nature of the Chemical Bond Formed with the Structural Metal Ion at the A9/G10.1 Motif Derived from Hammerhead Ribozymes
Y. Tanaka; Y. Kasai; S. Mochizuki; A. Wakisaka; E. H. Morita; C. Kojima; A. Toyozawa; Y. Kondo; M. Taki; Y. Takagi; A. Inoue; K. Yamasaki; K. Taira
J. Am. Chem. Soc, 126, 3, 744-752, 2004, Peer-reviwed
Scientific journal, English - Synthesis and antigen-binding properties of fluorescent labeled camel antibody
H. Hamada; R. Abe; M. Taki; H. Shinohara; T. Hohsaka; M. Sisido
Proc. Electrochem. Soc., 004-08(Chemical Sensors VI), 431-435, 2004
International conference proceedings, English - Site-directed incorporation of non-natural amino acid into Streptomyces xylanase
T. Ichikawa; A. Kuno; M. Taki; T. Hohsaka; M. Sisido; S. Kaneko; K. Taira; H. Kobayashi; T. Hasegawa
Nucleic Acids Res. Supl., 4, 161-162, 2004
International conference proceedings, English - Synthesis of a novel histidine analogue and its efficient incorporation into a protein in vivo
Y Ikeda; S Kawahara; M Taki; A Kuno; T Hasegawa; K Taira
PROTEIN ENGINEERING, 16, 9, 699-706, Sep. 2003, Peer-reviwed
Scientific journal, English - A direct and efficient synthesis method for dumbbell-shaped linear DNA using PCR in vitro
M. Taki; Y. Kato; M. Miyagishi; Y. Takagi; M. Sano; K. Taira
Nucleic Acids Res. Supl., 3, 191-192, 2003
International conference proceedings, English - Leucyl/Phenylalanyl (L/F)-tRNA-protein transferase-mediated N-terminal specific labelling of a protein in vitro
A. Kuno; M. Taki; K. Taira; T. Hasegawa
Nucleic Acids Res. Supl., 3, 259-260, 2003
International conference proceedings, English - Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs
M Taki; T Hohsaka; H Murakami; K Taira; M Sisido
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 124, 49, 14586-14590, Dec. 2002, Peer-reviwed
Scientific journal, English - Highly sensitive fluorescent nonnatural amino acids that can be incorporated into specific positions of streptavidin
M Taki; T Hohsaka; H Murakami; K Taira; M Sisido
PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, 177, 8-9, 1929-1930, Aug. 2002, Peer-reviwed
Scientific journal, English - A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin
M. Taki; T. Hohsaka; H. Murakami; A. Kuno; T. Hasegawa; K. Taira; M. Sisido
Nucleic Acids Res. Supl., 2, 203-204, 2002
International conference proceedings, English - A non-natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe
M Taki; T Hohsaka; H Murakami; K Taira; M Sisido
FEBS LETTERS, 507, 1, 35-38, Oct. 2001, Peer-reviwed
Scientific journal, English - Specific N-terminal biotinylation of a protein in vitro by a chemically modified tRNA(fmet) can support the native activity of the translated protein
M Taki; SY Sawata; K Taira
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 92, 2, 149-153, Aug. 2001, Peer-reviwed
Scientific journal, English - Site-specific cleavage of a protein via introducing a hydroxy acid derivative in the main chain by using four-base codon/anticodon pairs
M. Taki; T. Hohsaka; M. Sisido
Nucleic Acids Res. Supl, 1, 227-228, 2001
International conference proceedings, English - A novel immobilization method of an active protein via in vitro N-terminal specific incorporation system of nonnatural amino acids
M. Taki; S. Y. Sawata; K. Taira
Nucleic Acids Res. Supl, 1, 197-198, 2001
International conference proceedings, English - Langmuir films of amphiphilic [60]fullerene derivatives
Y Nakamura; M Taki; A Asami; S Inokuma; K Hiratani; K Taguchi; M Higuchi; J Nishimura
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 73, 7, 1615-1619, Jul. 2000, Peer-reviwed
Scientific journal, English - A chiral Eu3+-thienoyltrifluoroacetone complex on an avidin tetramer: luminescence and CD studies on the supramolecular protein-metal chelate complex
M Taki; H Murakami; M Sisido
CHEMICAL COMMUNICATIONS, 13, 1199-1200, 2000, Peer-reviwed
Scientific journal, English - Photophysical properties of various regioisomers of [60]fullerene-o-quinodimethane bisadducts
Y Nakamura; M Taki; S Tobita; H Shizuka; H Yokoi; K Ishiguro; Y Sawaki; J Nishimura
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1, 1, 127-130, Jan. 1999, Peer-reviwed
Scientific journal, English - [60]Fullerene (A(1),D-1)-bisadducts: CD spectra of enantiomers and diastereospecific synthesis
M Taki; Y Nakamura; H Uehara; M Sato; J Nishimura
ENANTIOMER, 3, 3, 231-239, 1998
Scientific journal, English - Selective functionalization on [60]fullerene governed by tether length
M Taki; S Sugita; Y Nakamura; E Kasashima; E Yashima; Y Okamoto; J Nishimura
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 119, 5, 926-932, Feb. 1997, Peer-reviwed
Scientific journal, English - Synthesis of polyesters containing the [60]fullerene moiety in the main chain
M Taki; S Takigami; Y Watanabe; Y Nakamura; J Nishimura
POLYMER JOURNAL, 29, 12, 1020-1022, 1997, Peer-reviwed
Scientific journal, English - [60]フラーレンへのオルトキノジメタン及びその類縁体の付加反応と生成物の性質
中村洋介; 瀧 真清; 西村 淳
季刊フラーレン, 5, 133-143, 1997
International conference proceedings, Japanese - フラーレンの化学の最近の進歩 その1 -[60]フラーレンへのオルトキノジメタンおよびその類縁体の付加反応・生成物の物性
中村洋介; 瀧 真清; 美濃和寿幸; 西村 淳
有機合成化学協会誌, 54, 574-579, 1996
International conference proceedings, Japanese - NOMENCLATURE OF [60]FULLERENE DERIVATIVES BY EDGE LABELING
Y NAKAMURA; M TAKI; J NISHIMURA
CHEMISTRY LETTERS, 8, 703-704, Aug. 1995, Peer-reviwed
Scientific journal, English - PHOTOREDUCTION OF WATER BY THE SYSTEM OF C-60-PLATINUM-METHYLVIOLOGEN
M IGARASHI; M FUKUDA; M TAKI; T TAGO; T MINOWA; Y OKADA; J NISHIMURA
FULLERENE SCIENCE AND TECHNOLOGY, 3, 1, 37-43, 1995, Peer-reviwed
Scientific journal, English
MISC
- Peptidic Targeted Covalent Inhibitors (TCIs) possessing Stability in Serum
松塚 洸樹; 勝木 陸; 瀧 真清
Last, Apr. 2024, PEPTIDE NEWSLETTER JAPAN, 132, 4-6, Peer-reviwed, Invited, Introduction scientific journal - PACIFICHEM2015今昔
瀧 真清
Apr. 2016, Peptide Newsletter Japan, 100(記念), 23-25, Japanese, Invited, Introduction other - L/F-転移酵素を用いた蛋白質N末端への機能性非天然アミノ酸導入
瀧 真清
2008, 生体機能関連化学部会Newsletter, 23, 3-6, Japanese, Introduction other - カリフォルニア工科大学における研究教育
瀧 真清
2008, Peptide Newsletter Japan, 67, 1, 11-13, Japanese, Introduction other - 気になった論文
瀧 真清
2007, 生命化学研究レター, 23, 2, 22-25, Japanese, Introduction other - 非天然アミノ酸の導入による蛋白質の蛍光ラベル法とその応用
宍戸 昌彦; 瀧 真清; 大槻高史; 芳坂貴弘
共立出版, 2006, 蛋白質核酸酵素, 51, 5, 399-407, Japanese, Introduction other, 0039-9450, 40007273470, AN00140437 - Position-specific incorporation of a fluorophore-quencher pair into a single protein through orthogonal 4-base codon/anticodon pairs.
M Taki; T Hohsaka; M Sisido
Mar. 2005, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 229, U567-U567, English, Summary international conference, 0065-7727, WOS:000235066602372 - 活性蛋白質の蛍光標識技術
瀧 真清; 宍戸 昌彦
2004, 化学, 59, 72-73, Japanese, Introduction other
Books and other publications
- Complementing Stability and Affinity of a Linear Peptidic Binder by Adapting Retro-Inverso to a Peptidic Covalent Binder, Peptide Science 2023
Koki Matsuzuka; Riku Katsuki; Masumi Taki
Joint work, p.81-82, The Japanese Peptide Society, Aug. 2024, 9784931541245 - Discovery and Characterization of a Short Peptide Possessing a Latent Warhead Undergoing Intramolecular Cyclization in the Aqueous Environment, Peptide Science 2023
Riku Katsuki; Masumi Taki
Joint work, p.27-28, The Japanese Peptide Society, Aug. 2024, 9784931541245 - Direct combinatorial screening of a target-specific covalent binding peptide: activation of the warhead reactivity in a pseudo-catalytic microenvironment, Peptide Science 2021
Yudai Tabuchi; Riku Katsuki; Yuji Ito; Masumi Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, Mar. 2022 - Reversal of covalent conjugation of benzoxaborole-modified targeted peptide by reduction, Peptide Science 2021
Takashi Abe; Yudai Tabuchi; Masumi Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, Mar. 2022 - Construct of cyclic peptide phage library containing covalent warhead, Peptide Science 2021
Riku Katsuki; Yudai Tabuchi; Masumi Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, Mar. 2022 - A solvatochromic binder obtained via an extended phage-display acts as a fluororeporter for the fragment-based drug discovery / Peptide Science 2020
R. Katsuki; T. Numayama; M. Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, Mar. 2021 - Chemo-enzymatic Synthesis of biologics-fused hybrid molecules via NEXT-A reaction / Peptide Science 2019
M. Taki
Scholarly book, English, Single work, The Japanese Peptide Society, Mar. 2020 - 「ファージディスプレイ法を利用した機能性ペプチドスクリーニング」, 医薬品開発における中分子領域（核酸医薬・ペプチド医薬）の開発戦略
谷田部 和貴; 田淵 雄大; 望月 和人; 瀧 真清
Scholarly book, Japanese, Joint work, 第3章第3節, 情報機構, 15 Oct. 2019, 9784865021769 - Combinatorially Screened Peptide as Targeted Covalent Binder / Peptide Science 2018
M. Taki
Scholarly book, English, Single work, The Japanese Peptide Society, Mar. 2019 - Construction of cryptand library via the Gp10 based-thioetherification (10BASEd-T) / Peptide Science 2016
K. Mochizuki; Y. Ito; M. Minami; M. Taki
English, Joint work, The Japanese Peptide Society, 2017 - Bioconjugation approach towards peptide-Fc fusion compounds / Peptide Science 2016
S. Hirasawa; M. Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, 2017 - Theoretical/Experimental Structural Analysis of Protein-Ligand Interaction between GST and its Fluorogenic Binder Created by the 10BASEd-T / Peptide Science 2015
K. Yahiro; T. Yamakoshi; J. Yang; S. Watanabe; M. Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, 2016 - Synthesis of Multicyclic Library for Finding Strong Target-Specific Binders / Peptide Science 2014
Y. Arai; M. Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, 2015 - Artificial Macrocycle as a Functional-Equivalent of Catalytic Antibody / Peptide Science 2014
H. Inoue; R. Asano; M. Taki
Scholarly book, English, Joint work, The Japanese Peptide Society, 2015 - Construction of a Peptide Expression Vector Library for Phenotypic Screening of Bioactive Peptides in Yeast / Peptide Science 2013
K. Fukunaga; M. Taki
English, Joint work, The Japanese Peptide Society, 2014 - Selection of Streptavidin-Binding Artificial Peptide Posessing Salicylic Acid Moiety via the 10BASEd-T on the Bacteriophage T7 / Peptide Science 2013
Y. Tokunaga; K. Fukunaga; T. Hatanaka; Y. Ito; M. Taki
English, Joint work, The Japanese Peptide Society, 2014 - An Artificial Molecule-Peptide Hybrid Discovered via the 10BASEd-T Binds to the Substrate-Binding Site of Glutathione S-Transferase / Peptide Science 2013
K. Fukunaga; T. Hatanaka; Y. Ito; M. Taki
English, Joint work, The Japanese Peptide Society, 2014 - Synthesis of N-terminal specific PET-probe labeling of peptides via the NEXT-A reaction / Peptide Science 2012
Y. Tokuda; H. Kimura; H. Saiki; M. Taki; H. Saji
English, Joint work, The Japanese Peptide Society, 2013 - 第2章「創薬システムエンジニアリング：NEXT-A法および10BASEd-T法の開発」, 総合コミュニケーション科学シリーズ ユニーク＆エキサイティング サイエンス２
瀧 真清
General book, Japanese, Joint work, 近代科学社, 2013 - Synthesis of cyclic peptides using the NEXT-A Reaction / Peptide Science 2010
T. Hamamoto; M. Sisido; T. Ohtsuki; M. Taki
English, Joint work, The Japanese Peptide Society, 2011 - 第4章「L/F-転移酵素による機能性非天然アミノ酸の蛋白質N末端への導入」酵素利用技術大系
瀧 真清; 宍戸 昌彦
Japanese, Joint work, NTS社, 2010 - N-Terminal Specific Point-Immobilization and Fluorescence Labeling of Peptide/Protein by the One-Pot NEXT-A Reaction / Peptide Science 2009
M. Taki; K. Ebisu; H. Kuroiwa; M. Sisido
English, Joint work, The Japanese Peptide Society, 2010 - 第2章「タンパク質工学の基礎」バイオプロセスハンドブック
瀧 真清; 宍戸 昌彦
Japanese, Joint work, NTS社, 2007 - Position-specific Incorporation of Blue-laser Excitable Fluorescent Amino Acids into Peptides and Proteins / Peptide Science 2005
M. Taki; Y. Suzuki; Y. Yamazaki; M. Sisido
English, Joint work, The Japanese Peptide Society, 2006 - Spectroscopic properties of some [60]fullerene-o-quinodimethane monoadducts and bisadducts
Y. Nakamura; M. Taki; S. Tobita; H. Shizuka; A. Mori; J. Nishimura
English, Joint work, K. M. Kadish and R. S. Ruoff, Electrochem. Soc. Inc., Pennington, 1997
Lectures, oral presentations, etc.
- BIOmolecular Targeted Covalent Inhibitor (bioTCI)
Masumi Taki
Invited oral presentation, English, Simposium Teknik Biomedis 2024 (@UGM, Indonesia), Invited
14 Nov. 2024 - Discovery and characterization of a short peptide possessing a latent warhead undergoing spontaneous intramolecular cyclization in the aqueous environment
Riku Katsuki; Masumi Taki
Oral presentation, English, The 60th Japanese Peptide Symposium
09 Nov. 2023 - 中分子共有結合薬剤 (bioTCI)：ペプチド型TCIの直接選択とアプタマーのTCI化
瀧 真清
Oral presentation, 第17回バイオ関連化学シンポジウム
08 Sep. 2023 - 中分子共有結合薬剤の基礎開発と医工産学連携の実例
瀧 真清
Public discourse, 第129回 電気通信大学 産学官連携センター 研究開発セミナー, Invited
09 Jun. 2023 - 日本の学会の現状：全2037の国内学会の定量解析と成功／失敗事例
瀧 真清
Public discourse, 第二回総合コミュニケーション科学学会 総会及び座談会, Invited
13 May 2023 - 中分子共有結合薬剤
瀧 真清
Public discourse, JAISTバイオ機能医工学研究領域セミナー, Invited
23 Jan. 2023 - Design, Selection, and Engineering of Targeted Hybrid-Middle Molecules via 10BASEd-T / NEXT-A Reactions
M. Taki
Oral presentation, English, PACIFICHEM2021, Honolulu, International conference
20 Dec. 2021 - Turn-on/keep-on fluctuated fluorescent molecules as targeted binders
Masumi Taki
Invited oral presentation, English, 第58回日本生物物理学会年会, Invited, Domestic conference
2020 - 小・中・高分子、何でもアリの分子標的型薬剤/医用材料開発
瀧 真清
Public discourse, Japanese, 第8回電通大先端化学セミナー, Invited, 電通大先端化学セミナー, Domestic conference
26 Nov. 2019 - Chemoenzymatic synthesis of biologics-fused hybrid molecules via NEXT-A reaction
M. Taki
Oral presentation, English, 56th Japanese Peptide Symposium (56JPS), 東京, Domestic conference
2019 - Turn-on / keep-on fluorescent molecules as targeted binders
M. Taki
Invited oral presentation, English, The third international workshop on symbiosis of biology and nanodevices, Invited, JSPS, 奈良, International conference
2019 - Combinatorially Screened Peptide as Targeted Covalent Binder
M. Taki
Oral presentation, English, ICBMBB2018, クアラルンプール, International conference
15 Aug. 2018 - 生体系と人工系の良いとこ取りをしたネオバイオ分子の創成と実験医学へ展開
瀧 真清
Public discourse, Japanese, 早稲田大学・先進理工学部（化学・生命化学科）セミナー, Invited, 早稲田大学, Domestic conference
10 Jul. 2018 - 難病を制する創薬に活用できるI科の技術についてお話ししよう
瀧 真清
Public discourse, Japanese, 産学官連携センターベンチャー支援部門セミナー（UEC)
09 Jan. 2018 - 創薬を指向したT7ファージウィルス上でのネオバイオ分子の創成
瀧 真清
Invited oral presentation, Japanese, 日本農芸化学会2018年度大会 シンポジウム, Invited, 日本農芸化学会, 名城大学, Domestic conference
2018 - Engineering Neobiologics for Drug-Discovery
M. Taki
Public discourse, English, ETH Zurich Seminar, Invited, スイス連邦工科大学（ETH Zurich）, Zurich市(スイス), International conference
13 Sep. 2017 - Creation of neobiological molecules via the 10BASEd-T for drug discovery
M. Taki
Public discourse, English, Polish Academy of Science Seminar, Invited, ポーランド科学アカデミー, International conference
08 Jun. 2017 - 生体系と人工系の良いとこ取りをしたネオバイオ分子の創成と実験医学への展開
瀧 真清
Invited oral presentation, Japanese, 新潟大学コアステーション「ユビキタスグリーンケミカルエネルギー連携教育研究センター」第７回研究シンポジウム, Invited, 新潟大学, http://chem.sc.niigata-u.ac.jp/~gc-center/170314_2.pdf, Domestic conference
14 Mar. 2017 - 創薬を指向したT7ファージウィルス上でのネオバイオ分子の創成と LC/MSによる反応解析
瀧 真清
Invited oral presentation, Japanese, 第５回 大学連携研究設備ネットワーク研究成果報告会 （共同開催：第２回 千葉質量分析懇談会） ～質量分析を中心とした最新の研究展開～, Invited, 千葉大学・西千葉キャンパス, http://www.cac.chiba-u.ac.jp/event/2017/170308.pdf, Domestic conference
08 Mar. 2017 - Turn-on and Color-changeable Fluorogenic Molecular Probe for Specific Protein Detection Created by the 10BASEd-T
Masumi Taki
Invited oral presentation, English, The IRAGO conference 2016, Invited, http://www.iragoconference.jp/, International conference
02 Nov. 2016 - Turn-on and color-changeable fluorogenic sensor created by the 10BASEd-T
M. Taki
Oral presentation, English, ICONAN2016, パリ市（フランス）, International conference
28 Sep. 2016 - Artificial Molecule Evolution via the 10BASEd-T
M. Taki
Public discourse, English, ETH Zurich Intergroup Seminar, Invited, スイス連邦工科大学（ETH Zurich）, Zurich市(スイス), International conference
26 Sep. 2016 - 創薬・診断を目指した10BASEd-T法の応用：ファージ上での人工分子の共進化
瀧 真清
Nominated symposium, Japanese, 第1回埼玉大学先端産業国際ラボラトリー 次世代抗体の有効活用ワークショップ, Invited, 埼玉大学, 埼玉県さいたま市, Domestic conference
21 Sep. 2016 - 人工分子コアの10BASEd-T法による進化
瀧 真清
Invited oral presentation, Japanese, 第16回日本蛋白質科学会年会ワークショップ, Invited, 日本蛋白質科学会, Domestic conference
09 Jun. 2016 - Evolution of artificial molecule core via the 10BASEd-T
M. Taki
Invited oral presentation, English, 有機物性連携セミナー（UEC,阪大, 分子研のジョイントセミナー）, Invited, 電通大, International conference
30 May 2016 - 進化分子工学（10BASEd-T法）による人工抗体代替物の創成
瀧 真清
Invited oral presentation, Japanese, 第11回理研「バイオものづくり」シンポジウム, Invited, 理化学研究所, 埼玉県和光市, Domestic conference
04 Mar. 2016 - Cryptand library construction via the 10BASEd-T for specific binding toward a cancer-related protein
K. Mochizuki; M. Taki
Oral presentation, English, PACIFICHEM2015, Honolulu, International conference
17 Dec. 2015 - Turn-on and color-changeable fluorogenic sensor created by the 10BASEd-T
M. Taki; H. Inoue; K. Mochizuki
Oral presentation, English, 52nd Japanese Peptide Symposium, 平塚
18 Nov. 2015 - 人工分子の進化によるものづくり（創薬システム工学を中心に）
瀧 真清
Invited oral presentation, Japanese, 第101回 複合材料懇話会, Invited, 北関東産学官研究会, 群馬大学（桐生キャンパス）, Domestic conference
04 Sep. 2015 - 10BASEd-T法による人工分子コアの進化
瀧 真清
Invited oral presentation, Japanese, 第47回若手ペプチド夏の勉強会, Invited, 塩尻市, Domestic conference
11 Aug. 2015 - Construction of peptide/protein-hybrid molecules via the NEXT-A and the 10BASEd-T reaction for PET imaging
Masumi Taki
Invited oral presentation, English, The 9th ICME International Conference on Complex Medical Engineering (CME 2015), Invited, 岡山市, International conference
20 Jun. 2015 - Artificial Molecule Evolution via the 10BASEd-T
Masumi Taki
Invited oral presentation, English, IMS Asian International Symposium (Supramolecular Dynamics at the Interface of Chemistry and Biology), Invited, 分子科学研究所, 岡崎（愛知県）, International conference
12 Jun. 2015 - Drug-discovery systems engineering: construction of peptide/protein hybrid molecules via the NEXT-A and/or the 10BASEd-T reaction
M. Taki
Invited oral presentation, English, BIT's 7th annual congress of industrial biotechnology (ibio)-2014, Invited, International conference
27 Apr. 2014 - Cancer detection and cure: antibody drugs and (hybrid) peptides as antibody substitutes
M. Taki
Public discourse, English, The second conference on interdisciplinary research in traditional medicine and modern medical bioscience, Invited, Nanjing Med. Univ., Nanjing, International conference
24 Apr. 2014 - Position-specific modification of nucleic acids/peptides/proteins toward pharmaceutical systems engineering
M. Taki
Public discourse, English, 南京医科大学学術講演（セミナー）, Invited, International conference
23 Apr. 2014 - 創薬システムエンジニアリング：NEXT-A法および10BASEd-T法の開発
瀧 真清
Public discourse, Japanese, 群馬大学 H25年度夏期複合材料研究会, Invited, Domestic conference
21 Jul. 2013 - NEXT-A反応による蛋白質/ペプチドの迅速標識
瀧 真清
Invited oral presentation, Japanese, ハイペップ研究所 第11回紅葉ワークショップ, ハイペップ研究所
Dec. 2012 - Introduction of functional amino acids at the N-terminus of peptide/protein by the NEXT-A (N-Terminal EXtension with Transferase and ARS) reaction
M. Taki
Public discourse, English, コペンハーゲン大学セミナー, Invited, コペンハーゲン大学, コペンハーゲン大学、コペンハーゲン市(デンマーク), International conference
Sep. 2011 - Synthesis of cyclic peptides/proteins using the NEXT-A/Cyclization reaction
M. Taki; T. Hamamoto; M. Sisido
Public symposium, English, 4th European Conference on Chemistry for Life Sciences (4ECCLS), ブダペスト市（ハンガ リー）
Sep. 2011 - NEXT-A反応を用いた蛋白質N末端への機能性非天然アミノ酸導入
瀧 真清
Invited oral presentation, Japanese, 第11回日本蛋白質科学会年会ワークショップ, 日本蛋白質科学会
Jun. 2011 - N-terminal specific 18F-labeling of peptides and proteins
H. Kuroiwa; M. Sisido; M. Taki
Public symposium, English, PACIFICHEM2010
Dec. 2010 - Synthesis of cyclic peptides using the NEXT-A reaction
M. Taki; T. Hamamoto; M. Sisido
Public symposium, English, PACIFICHEM2010, ホノルル市（アメリカ）
Dec. 2010 - Introduction of functional amino acids at the N-terminus of peptide/protein
M. Taki
Invited oral presentation, English, ハイペップ研究所 第4回HiPep沖縄国際ワークショップ公開講演会, ハイペップ研究所
Jul. 2010 - N-TERMINAL SPECIFIC POINT-IMMOBILIZATION OF PEPTIDE/PROTEIN BY THE ONE-POT NEXT-A REACTION
M. Taki; K. Ebisu; M. Sisido
Public symposium, English, International Conference of 46th Japanese Peptide Symposium and 5th Peptide Engineering Meeting, 小倉
Nov. 2009 - The NEXT-A reaction
M. Taki; H. Kuroiwa; M. Sisido
Public symposium, English, 第6回国際核酸化学シンポジウム, 高山
Sep. 2009 - Tag-Free N-Terminal Specific Immobilization of Lectin via the NEXT-A Reaction for Sugar Detection
M. Taki; K. Ebisu; M. Sisido
Public symposium, English, EuroAnalysis2009, インスブルック市（オーストリア）
Sep. 2009 - An invited lecture-Regiospecific modifications of (bio)macromolecules
M. Taki
Public discourse, English, ポーランド科学アカデミーセミナー, Invited, ポーランド科学アカデミー, International conference
Sep. 2009 - L/F-transferaseを用いた蛋白質N末端特異的な蛍光および陽電子放射断層撮影(PET)プローブ標識
瀧 真清
Invited oral presentation, Japanese, 大阪大学蛋白質研究所セミナー：蛋白質合成法の最近の進歩と生命科学, 大阪大学蛋白質研究所, Domestic conference
Sep. 2008 - Regiospecific modifications of (bio)macromolecules
瀧 真清
Invited oral presentation, Japanese, 第40回ペプチド夏の勉強会, Domestic conference
Aug. 2007 - 4塩基コドン／アンチコドン対を用いた哺乳動物 生細胞内での遺伝暗号の拡張
瀧 真清; 松下 治朗; 宍戸 昌彦
Invited oral presentation, Japanese, 第7回日本蛋白質科学会年会, 日本蛋白質科学会
May 2007 - Position-specific incorporation of blue-laser excitable fluorescent amino acids into peptides
M. Taki; Y. Yamazaki; M. Sisido
Public symposium, English, InternationalConference of 43rd Japanese Peptide Symposium and 4th Peptide Engineering Meeting, 横浜
Nov. 2006
Courses
Affiliated academic society
Research Themes
- T型／H型ペプチド構造を持つコバレントバインダーによる標的蛋白質の不可逆的阻害
瀧 真清
Apr. 2021 - Mar. 2024 - 血液凝固機能を阻害する非天然型DNAアプタマー薬剤の構造とヌクレアーゼ耐性機構
渡辺 信一
Apr. 2021 - Mar. 2024 - 表面プラズモン共鳴小型半導体化学量センサの実用検証補助事業
菅 哲朗
財団法人 JKA, 機械振興補助事業, Coinvestigator
2023 - 2024 - 半導体SPRセンサによるコロナウイルスのリアルタイム分布可視化技術開発
菅 哲朗
JST, A-STEP 産学共同（育成型）：with/postコロナにおける社会変革への寄与が期待される研究開発課題への支援, 国立大学法人電気通信大学, Coinvestigator, 本課題では、コロナウイルスのリアルタイム検出に適用可能な小型化学量センサを開発する。提案者がシーズ技術を有する半導体表面プラズモン共鳴(SPR)センサに対し、ウイルスを想定した検出感度・分解能の評価検証を行い、コロナウイルスの高感度センサを小型で実現するための実現性見極めを行う。小型センサが実現すれば、人の行動環境中に多数配置して、感染を経ずに空気中のウイルスをその場リアルタイムで検知できる。ウイルスの確認に要する時間の圧倒的短縮、空間分解能の飛躍的増大というイノベーションが生まれる。市販のSPR装置をベンチマークとし、提案センサの性能評価と疑似ウイルス検出能力を確認し、実現可能性を判断する。
Mar. 2021 - Mar. 2022 - ウイルス可視化のためのプラズモニック半導体センサ
菅 哲朗
2020 - 2022 - 蛍光分子の2段階成熟による環境応答性センサー型分子の取得
瀧 真清
Principal investigator
Apr. 2017 - Mar. 2020 - Chemical Expansion of the Central Dogma towards Synthetic Microorganisms
SISIDO Masahiko; OHTSUKI Takashi; TAKI Masumi
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Okayama University, Grant-in-Aid for Scientific Research (S), This research project aims to expand molecules like amino acids, nucleic acids and proteins constitute the protein biosynthesizing system to synthesize novel type of proteins with artificial functions. The final goal is to create synthetic microorganisms that live with 21 type of amino acids. During the 5 year we obtained the following results. (1) Some nonnatural amino acids were successfully charged onto a specific tRNA by the use of a peptide nucleic acid (PNA) that is complementary to the tRNA. The PNA-assisted aminoacylation mold be carried out in an E.coli in vitro protein biosynthesizing system to create a protein incorporated with the nonnatural amino add. (2) tRNAs and an elongation faint Tu. (EF-Tu) were expanded to bring large-sized nonnatural amino adds into E.col ribosome and subsequently to a protein. (3) An orthogonal set of aminoacyl tRNAsynthetase (ARS) and tRNA was screened to bring some nonnatural amino acids into proteins in living mammalian cells. (4) Finally these expanded molecular systems were introduced into mammalian cells and the synthesis of proteins containing nonnatural amino acids was detected, although its quantity was very small. In the last year, an attempt was made to apply these techniques to attach fluorescent amino acids to peptides and proteins. The fluorescently labeled peptides were screened to dimmer those that bind to cancer cells or to cancer-cell specific proteins., 15101008
2003 - 2007
Industrial Property Rights
- 中和可能コバレントドラッグ
Patent right, 田淵 雄大, ヤン ジェイ, 瀧 真清, 特願2021-576738, Date applied: 28 Oct. 2021, 7029760, Date issued: 24 Feb. 2022 - マクロ環分子およびそのスクリーニング方法
Patent right, 瀧 真清, 2015-243334, Date applied: 14 Dec. 2015, 6622079, Date issued: 29 Nov. 2019 - ペプチド又はタンパク質へのフッ素含有アミノ酸導入法
Patent right, 齊木 秀和, 小関 英一, 長谷川 雪憲, 瀧 真清, PCT/JP2013/076025, Date applied: 26 Sep. 2013, 株式会社島津製作所, 国立大学法人電気通信大学, WO2015/045052, Date announced: 02 Apr. 2015, 6099756, Date issued: 03 Mar. 2017 - ペプチド又はタンパク質へのフッ素導入法
Patent right, 斉木秀和, 小関英一, 瀧 真清, 宍戸昌彦, 特願2012-157012 PCT/JP2013/68829, Date applied: 10 Jul. 2013, 5945324, Date issued: 03 Jun. 2016 - 目的タンパク質または目的ペプチドにアミノ酸を導入する方法
Patent right, 特願2008-135410および2008-181303 (2008;分割出願),後者特許取得第5279379号(2013), Date applied: 2008, 瀧 真清、宍戸 昌彦, Date announced: 2008, 5279378, Date issued: 31 May 2013 - 化学修飾されたペプチドを有するファージおよびその製造方法
Patent right, 瀧 真清, 福永 圭祐, 特願2012-287045, Date applied: 28 Dec. 2012, The University of Electro-Communications - ダンベル型DNAの効率的な製造方法
Patent right, 特願2005-512987(2004), Date applied: 2004, 高木 康臣、多比良 和誠、瀧 真清、加藤 義雄、宮岸 真, 特許取得 第4517061号(2010),米国特許取得 第7972816号(2011), Date issued: 2010 - 蛍光性アミノ酸誘導体
Patent right, PCT出願JP2006/311561 (2006), Date applied: 2005, 瀧 真清、宍戸 昌彦, 特願2005-171019, Date announced: 2005, 特許取得 第4392502号(2009),米国特許取得 第7928236号(2011), Date issued: 2009 - 新規蛍光性アミノ酸誘導体およびその製造方法
Patent right, PCT出願JP2007/063261 (2007), Date applied: 2006, 瀧 真清、宍戸 昌彦, 特願2006-184294 (2006), Date announced: 2006, 特許公開2008-13456,米国特許取得 第7847098号(2010), Date issued: 2008
