Kitada Nobuo

Coordinated Center for UEC Research FacilitiesProject/Specially Appointed Assistant Professor
Researcher Information


  • PhD (Science), The University of Electro-Communications, Sep. 2018
Research Activity Information


  • Synthesis and anti-tumor activities in human leukemia-derived cells of polyenylpyrroles with a methyl group at the conjugated polyene terminus
    Tomoya Higashi; Chihiro Yoshida; Yoshifumi Hachiro; Chihiro Nakata; Azusa Takechi; Takuya Yagi; Kazuya Miyashita; Nobuo Kitada; Rika Obata; Takashi Hirano; Takahiko Hara; Shojiro A. Maki
    Bioorganic & Medicinal Chemistry Letters, Elsevier BV, 95, 129471-129471, Oct. 2023
    Scientific journal
  • Identification of compounds that preferentially suppress the growth of T‐cell acute lymphoblastic leukemia‐derived cells
    Kazuya Miyashita; Takuya Yagi; Noritaka Kagaya; Azusa Takechi; Chihiro Nakata; Risa Kanda; Hideko Nuriya; Kosuke Tanegashima; Shota Hoyano; Fumiya Seki; Chihiro Yoshida; Yoshifumi Hachiro; Tomoya Higashi; Nobuo Kitada; Takashi Toya; Takeshi Kobayashi; Yuho Najima; Susumu Goyama; Shojiro A. Maki; Toshio Kitamura; Noriko Doki; Kazuo Shin‐ya; Takahiko Hara
    Cancer Science, Wiley, 114, 10, 4032-4040, 31 Jul. 2023, Abstract

    T‐cell acute lymphoblastic leukemia (T‐ALL) is one of the most frequently occurring cancers in children and is associated with a poor prognosis. Here, we performed large‐scale screening of natural compound libraries to identify potential drugs against T‐ALL. We identified three low‐molecular‐weight compounds (auxarconjugatin‐B, rumbrin, and lavendamycin) that inhibited the proliferation of the T‐ALL cell line CCRF‐CEM, but not that of the B lymphoma cell line Raji in a low concentration range. Among them, auxarconjugatin‐B and rumbrin commonly contained a polyenyl 3‐chloropyrrol in their chemical structure, therefore we chose auxarconjugatin‐B for further analyses. Auxarconjugatin‐B suppressed the in vitro growth of five human T‐ALL cell lines and two T‐ALL patient‐derived cells, but not that of adult T‐cell leukemia patient‐derived cells. Cultured normal T cells were several‐fold resistant to auxarconjugatin‐B. Auxarconjugatin‐B and its synthetic analogue Ra#37 depolarized the mitochondrial membrane potential of CCRF‐CEM cells within 3 h of treatment. These compounds are promising seeds for developing novel anti‐T‐ALL drugs.
    Scientific journal
  • In vivo optical imaging of tumor stromal cells with hypoxia‐inducible factor activity
    Hitomi Miyabara; Ryuichiro Hirano; Shigeaki Watanabe; John Clyde Co Soriano; Hitomi Watanabe; Takahiro Kuchimaru; Nobuo Kitada; Tetsuya Kadonosono; Shojiro A. Maki; Gen Kondoh; Shinae Kizaka‐Kondoh
    Cancer Science, Wiley, 114, 10, 3935-3945, 22 Jul. 2023, Abstract

    Tumors contain various stromal cells, such as immune cells, endothelial cells, and fibroblasts, which contribute to the development of a tumor‐specific microenvironment characterized by hypoxia and inflammation, and are associated with malignant progression. In this study, we investigated the activity of intratumoral hypoxia‐inducible factor (HIF), which functions as a master regulator of the cellular response to hypoxia and inflammation. We constructed the HIF activity‐monitoring reporter gene hypoxia‐response element‐Venus‐Akaluc (HVA) that expresses the green fluorescent protein Venus and modified firefly luciferase Akaluc in a HIF activity‐dependent manner, and created transgenic mice harboring HVA transgene (HVA‐Tg). In HVA‐Tg, HIF‐active cells can be visualized using AkaBLI, an ultra‐sensitive in vivo bioluminescence imaging technology that produces an intense near‐infrared light upon reaction of Akaluc with the D‐luciferin analog AkaLumine‐HCl. By orthotopic transplantation of E0771, a mouse triple negative breast cancer cell line without a reporter gene, into HVA‐Tg, we succeeded in noninvasively monitoring bioluminescence signals from HIF‐active stromal cells as early as 8 days after transplantation. The HIF‐active stromal cells initially clustered locally and then spread throughout the tumors with growth. Immunohistochemistry and flow cytometry analyses revealed that CD11b+F4/80+ macrophages were the predominant HIF‐active stromal cells in E0771 tumors. These results indicate that HVA‐Tg is a useful tool for spatiotemporal analysis of HIF‐active tumor stromal cells, facilitating investigation of the roles of HIF‐active tumor stromal cells in tumor growth and malignant progression.
    Scientific journal
  • Creation of artificial luciferase 60s from sequential insights and their applications to bioassays
    Sung-Bae Kim; Tadaomi Furuta; Nobuo Kitada; Shojiro A. Maki
    Sensors, MDPI AG, 23, 14, 6376-6376, Jul. 2023, Peer-reviwed, In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences. Through compensating for the missing peptide blocks in the alignment, 10 sibling sequences were artificially fabricated and named from ALuc55 to ALuc68. The phylogenetic analysis showed that the new ALucs formed an independent branch that was genetically isolated from other natural marine luciferases. The new ALucs successfully survived and luminesced with native coelenterazine (nCTZ) and its analogs in living mammalian cells. The results showed that the bioluminescence (BL) intensities of the ALucs were interestingly proportional to the length of the appended peptide blocks. The computational modeling revealed that the appended peptide blocks created a flexible region near the active site, potentially modulating the enzymatic activities. The new ALucs generated various colors with maximally approximately 90 nm redshifted BL spectra in orange upon reaction with the authors’ previously reported 1- and 2-series coelenterazine analogs. The utilities of the new ALucs in bioassays were demonstrated through the construction of single-chain molecular strain probes and protein fragment complementation assay (PCA) probes. The success of this study can guide new insights into how we can engineer and functionalize marine luciferases to expand the toolbox of optical readouts for bioassays and molecular imaging.
    Scientific journal, English
  • Coelenterazine indicators for the specific imaging of human and bovine serum albumins
    Sung-Bae Kim; Genta Kamiya; Tadaomi Furuta; Nobuo Kitada; Shojiro A
    Sensors, MDPI AG, 23, 13, 6020, Jun. 2023, Peer-reviwed, Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitatively illuminate human and bovine serum albumins (HSA and BSA) with high specificity. Their functional groups were chemically modified to specifically emit luminescence with HSA and BSA. The CTZ indicators were characterized by assaying the most abundant serum proteins and found that the CTZ indicators S6 and S6h were highly specific to HSA and BSA, respectively. Their colors were dramatically converted from blue, peaked at 480 nm, to yellowish green, peaked at 535 nm, according to the HSA–BSA mixing ratios, wherein the origins and mixing levels of the albumins can be easily determined by their colors and peak positions. The kinetic properties of HSA and BSA were investigated in detail, confirming that the serum albumins catalyze the CTZ indicators, which act as pseudo-luciferases. The catalytic reactions were efficiently inhibited by specific inhibitors, blocking the drug-binding sites I and II of HSA and BSA. Finally, the utility of the CTZ indicators was demonstrated through a quantitative imaging of the real fetal bovine serum (FBS). This study is the first example to show that the CTZ indicators specify HSA and BSA with different colors. This study contributes to the expansion of the toolbox of optical indicators, which efficiently assays serum proteins in physiological samples. Considering that these CTZ indicators immediately report quantitative optical signals with high specificity, they provide solutions to conventional technical hurdles on point-of-care assays of serum albumins.
    Scientific journal, English
  • Bright Molecular Strain Probe Templates for Reporting Protein–Protein Interactions
    Sung-Bae Kim; Tadaomi Furuta; Genta Kamiya; Nobuo Kitada; Ramasamy Paulmurugan; Shojiro A. Maki
    Sensors, MDPI AG, 23, 7, 3498-3498, 27 Mar. 2023, Imaging protein–protein interactions (PPIs) is a hot topic in molecular medicine in the postgenomic sequencing era. In the present study, we report bright and highly sensitive single-chain molecular strain probe templates which embed full-length Renilla luciferase 8.6-535SG (RLuc86SG) or Artificial luciferase 49 (ALuc49) as reporters. These reporters were deployed between FKBP-rapamycin binding domain (FRB) and FK506-binding protein (FKBP) as a PPI model. This unique molecular design was conceptualized to exploit molecular strains of the sandwiched reporters appended by rapamycin-triggered intramolecular PPIs. The ligand-sensing properties of the templates were maximized by interface truncations and substrate modulation. The highest fold intensities, 9.4 and 16.6, of the templates were accomplished with RLuc86SG and ALuc49, respectively. The spectra of the templates, according to substrates, revealed that the colors are tunable to blue, green, and yellow. The putative substrate-binding chemistry and the working mechanisms of the probes were computationally modeled in the presence or absence of rapamycin. Considering that the molecular strain probe templates are applicable to other PPI models, the present approach would broaden the scope of the bioassay toolbox, which harnesses the privilege of luciferase reporters and the unique concept of the molecular strain probes into bioassays and molecular imaging.
    Scientific journal
  • Bioluminescent imaging systems boosting near-infrared signals in mammalian cells
    Sung-Bae Kim; Tadaomi Furuta; Yuki Ohmuro-Matsuyama; Nobuo Kitada; Ryo Nishihara; Shojiro A. Maki
    Photochemical & Photobiological Sciences, Springer Science and Business Media LLC, 22, 6, 1267-1278, 03 Feb. 2023
    Scientific journal
  • S-Series Coelenterazine-Driven Combinatorial Bioluminescence Imaging Systems for Mammalian Cells
    Genta Kamiya; Nobuo Kitada; Tadaomi Furuta; Takashi Hirano; Shojiro A. Maki; Sung-Bae Kim
    International Journal of Molecular Sciences, MDPI AG, 24, 2, 1420-1420, 11 Jan. 2023, A unique combinatorial bioluminescence (BL) imaging system was developed for determining molecular events in mammalian cells with various colors and BL intensity patterns. This imaging system consists of one or multiple reporter luciferases and a series of novel coelenterazine (CTZ) analogues named “S-series”. For this study, ten kinds of novel S-series CTZ analogues were synthesized and characterized concerning the BL intensities, spectra, colors, and specificity of various marine luciferases. The characterization revealed that the S-series CTZ analogues luminesce with blue-to-orange-colored BL spectra with marine luciferases, where the most red-shifted BL spectrum peaked at 583 nm. The colors completed a visible light color palette with those of our precedent C-series CTZ analogues. The synthesized substrates S1, S5, S6, and S7 were found to have a unique specificity with marine luciferases, such as R86SG, NanoLuc (shortly, NLuc), and ALuc16. They collectively showed unique BL intensity patterns to identify the marine luciferases together with colors. The marine luciferases, R86SG, NLuc, and ALuc16, were multiplexed into multi-reporter systems, the signals of which were quantitatively unmixed with the specific substrates. When the utility was applied to a single-chain molecular strain probe, the imaging system simultaneously reported three different optical indexes for a ligand, i.e., unique BL intensity and color patterns for identifying the reporters, together with the ligand-specific fold intensities in mammalian cells. This study directs a new combinatorial BL imaging system to specific image molecular events in mammalian cells with multiple optical indexes.
    Scientific journal
  • C-Series Coelenterazine-Driven Bioluminescence Signature Imaging
    Genta Kamiya; Nobuo Kitada; Tadaomi Furuta; Takashi Hirano; Shojiro Maki; Sung Bae Kim
    International Journal of Molecular Sciences, MDPI AG, 23, 21, 13047-13047, 27 Oct. 2022, The present study introduces a unique BL signature imaging system with novel CTZ analogues named “C-series.” Nine kinds of C-series CTZ analogues were first synthesized, and BL intensity patterns and spectra were then examined according to the marine luciferases. The results show that the four CTZ analogues named C3, C4, C6, and C7, individually or collectively luminesce with completely distinctive BL spectral signatures and intensity patterns according to the luciferases: Renilla luciferase (RLuc), NanoLuc, and artificial luciferase (ALuc). The signatural reporters were multiplexed into a multi-reporter system comprising RLuc8.6-535SG and ALuc16. The usefulness of the signatural reporters was further determined with a multi-probe system that consists of two single-chain probes embedding RLuc8 and ALuc23. This study is a great addition to the study of conventional bioassays with a unique methodology, and for the specification of each signal in a single- or multi-reporter system using unique BL signatures and patterns of reporter luciferases.
    Scientific journal
  • Multiplex quadruple bioluminescent assay system
    Genta Kamiya; Nobuo Kitada; Shojiro Maki; Sung Bae Kim
    Scientific Reports, 12, 17485, Oct. 2022, Peer-reviwed, Alucとウミホタル発光基質(K-シリーズ)の組み合わせで,多色化に成功しており,それを利用して,多重生物発光系の活性標識システムを構築した.
    Scientific journal, English
  • Quantum Yield of Near-Infrared Bioluminescence with Firefly Luciferin Analog: AkaLumine
    Ryohei Ono; Keita Osawa; Yutaka Takahashi; Yoshifumi Noguchi; Nobuo Kitada; Ryohei Saito-Moriya; Takashi Hirano; Shojiro A. Maki; Keisei Shibata; Hidefumi Akiyama; Ken-ichiro Kanno; Hideyuki Itabashi; Miyabi Hiyama
    Journal of Photochemistry and Photobiology A: Chemistry, Elsevier BV, 434, 114270-114270, Sep. 2022, Peer-reviwed
    Scientific journal
  • Bioluminescence imaging using d -luciferin and its analogs for visualizing Bdnf expression in living mice; different patterns of bioluminescence signals using distinct luciferase substrates
    Mamoru Fukuchi; Satoru Mitazaki; Ryohei Saito-Moriya; Nobuo Kitada; Shojiro A Maki; Hironori Izumi; Hisashi Mori
    The Journal of Biochemistry, Oxford University Press (OUP), 01 Sep. 2022, Abstract

    Brain-derived neurotrophic factor (BDNF) plays a crucial role in numerous brain functions, including memory consolidation. Previously, we generated a Bdnf-Luciferase transgenic (Bdnf-Luc) mouse strain to visualize changes in Bdnf expression using in vivo bioluminescence imaging (BLI). We successfully visualized activity-dependent Bdnf induction in living mouse brains using a d-luciferin analog, TokeOni, which distributes to the brain and produces near-infrared bioluminescence. In this study, we compared the patterns of bioluminescence signals within the whole body of the Bdnf-Luc mice produced by d-luciferin, TokeOni, and seMpai, another d-luciferin analog that produces a near-infrared light. As recently reported, hepatic background signals were observed in wild-type mice when using TokeOni. Bioluminescence signals were strongly observed from the region containing the liver when using d-luciferin and TokeOni. Additionally, we detected signals from the brain when using TokeOni. Compared with d-luciferin and TokeOni, signals were widely detected in the whole body of Bdnf-Luc mice by seMpai. The signals produced by seMpai were strong in the regions containing skeletal muscles in particular. Taken together, the patterns of bioluminescence signals in Bdnf-Luc mice vary when using different luciferase substrates. Therefore, the expression of Bdnf in tissues and organs of interest could be visualized by selecting an appropriate substrate.
    Scientific journal
  • Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions
    Haruhisa Ogawa; Ryohei Ono; Yoshifumi Noguchi; Nobuo Kitada; Ryohei Saito‐Moriya; Shojiro A. Maki; Hidefumi Akiyama; Hideyuki Itabashi; Miyabi Hiyama
    Photochemistry and Photobiology, Wiley, 97, 5, 1016-1022, Sep. 2021, Peer-reviwed, AkaLumine hydrochloride, named TokeOni, is one of the firefly luciferin analogs, and its reaction with firefly luciferase produces near-infrared (NIR) bioluminescence. Prior to studying the bioluminescence mechanism, basic knowledge about the chemical structures, electronic states, and absorption properties of TokeOni at various pH values of solution has to be acquired. In this paper, the absorption spectra for TokeOni and AkaLumine at pH 2-10 were measured. Density functional theory (DFT) calculations, time-dependent DFT calculations, and the vibrational analyses were carried out. The absorption spectra indicate that the chemical forms of TokeOni in solutions are same as those of AkaLumine. The peaks at pH 7-10 in the absorption spectra correspond to the excitation from the ground state of a carboxylate anion of AkaLumine, the peak at pH 2 corresponds to the excitation from the ground state of a carboxylate anion with an N-protonated thiazoline ring and N-protonated dimethylamino group of AkaLumine, and the peak at pH 4 corresponds to the excitation from the ground state of a carboxylate anion with an N-protonated thiazoline ring of AkaLumine.
    Scientific journal, English
  • Development of phenyl oligoene-type firefly luciferin analogues with extended π-electronic conjugation for near-infrared bioluminescence
    Genta Kamiya; Nobuo Kitada; Ryohei Saito-Moriya; Rika Obata; Satoshi Iwano; Atsushi Miyawaki; Takashi Hirano; Shojiro Maki
    Chemistry Letters, The Chemical Society of Japan, 20 May 2021
    Scientific journal
  • Synthesis of polyenylpyrrole derivatives with selective growth inhibitory activity against T-cell acute lymphoblastic leukemia cells
    Chihiro Yoshida; Tomoya Higashi; Yoshifumi Hachiro; Yuki Fujita; Takuya Yagi; Azusa Takechi; Chihiro Nakata; Kazuya Miyashita; Nobuo Kitada; Ryohei Saito; Rika Obata; Takashi Hirano; Takahiko Hara; Shojiro A. Maki
    Bioorganic & Medicinal Chemistry Letters, Elsevier BV, 37, 127837-127837, Apr. 2021
    Scientific journal
  • How to Select Firefly Luciferin Analogues for In Vivo Imaging
    Ryohei Saito-Moriya; Jun Nakayama; Genta Kamiya; Nobuo Kitada; Rika Obata; Shojiro A. Maki; Hiroshi Aoyama
    International Journal of Molecular Sciences, MDPI AG, 22, 4, 1848-1848, 12 Feb. 2021, Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.
    Scientific journal
  • Color-tunable bioluminescence imaging portfolio for cell imaging
    Shota Tamaki; Nobuo Kitada; Masahiro Kiyama; Rika Fujii; Takashi Hirano; Sung Bae Kim; Shojiro Maki
    Scientific Reports, Springer Science and Business Media LLC, 11, 1, 26 Jan. 2021, Abstract

    The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the whole color palette in the visible region and shows red-shifted BL with a marine luciferase: for example, Renilla luciferase 8 (RLuc8) and Artificial Luciferase 16 (ALuc16) show 187 nm- and 105 nm-redshifted spectra, respectively, by simply replacing the substrate CTZ with 1d. The optical properties of the new CTZ analogues were investigated such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to ALucs and are completely dark with RLuc derivatives, and 3d is highly specific to only NanoLuc. We further determined the theoretical background of the red-shifted BL maximum wavelengths (λBL) values according to the extended π conjugation of the CTZ backbone using Density Functional Theory (DFT) calculations. This color-tunable BL imaging system provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.
    Scientific journal
  • High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai.
    Jun Nakayama; Ryohei Saito; Yusuke Hayashi; Nobuo Kitada; Shota Tamaki; Yuxuan Han; Kentaro Semba; Shojiro A Maki
    International journal of molecular sciences, 21, 21, 24 Oct. 2020, True, Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.
    Scientific journal, English
  • Development of near-infrared firefly luciferin analogue reacted with wild-type and mutant luciferases.
    Nobuo Kitada; Ryohei Saito; Rika Obata; Satoshi Iwano; Kazuma Karube; Atsushi Miyawaki; Takashi Hirano; Shojiro A Maki
    Chirality, 32, 7, 922-931, Jul. 2020, True, Interestingly, only the D-form of firefly luciferin produces light by luciferin-luciferase (L-L) reaction. Certain firefly luciferin analogues with modified structures maintain bioluminescence (BL) activity; however, all L-form luciferin analogues show no BL activity. To this date, our group has developed luciferin analogues with moderate BL activity that produce light of various wavelengths. For in vivo bioluminescence imaging, one of the important factors for detection sensitivity is tissue permeability of the number of photons emitted by L-L reaction, and the wavelengths of light in the near-infrared (NIR) range (700-900 nm) are most appropriate for the purpose. Some NIR luciferin analogues by us had performance for in vivo experiments to make it possible to detect photons from deep target tissues in mice with high sensitivity, whereas only a few of them can produce NIR light by the L-L reactions with wild-type luciferase and/or mutant luciferase. Based on the structure-activity relationships, we designed and synthesized here a luciferin analogue with the 5-allyl-6-dimethylamino-2-naphthylethenyl moiety. This analogue exhibited NIR BL emissions with wild-type luciferase (λmax = 705 nm) and mutant luciferase AlaLuc (λmax = 655 nm).
    Scientific journal, English
  • Toward bioluminescence in the near-infrared region: Tuning the emission wavelength of firefly luciferin analogues by allyl substitution
    Nobuo Kitada; Tsuyoshi Saitoh; Yuma Ikeda; Satoshi Iwano; Rika Obata; Haruki Niwa; Takashi Hirano; Atsushi Miyawaki; Koji Suzuki; Shigeru Nishiyama; Shojiro A. Maki
    Tetrahedron Letters, 59, 12, 2018, Peer-reviwed
    Scientific journal
  • An allylated firefly luciferin analogue with luciferase specific response in living cells
    Ikeda, Yuma; Saitoh, Tsuyoshi; Niwa, Kazuki; Nakajima, Takahiro; Kitada, Nobuo; Maki, Shojiro A.; Sato, Moritoshi; Citterio, Daniel; Nishiyama, Shigeru; Suzuki, Koji
    Chem. Commun., The Royal Society of Chemistry, 54, 2018, Peer-reviwed
    Scientific journal
  • Aspiration Risk Detection Using Oral Administration of Fluorescent Food - Preliminary Experiments Using Meat Phantoms
    Takahiro Suzuki; Ryohei Saito; Nobuo Kitada; Takuji Koike; Shojiro Maki; Yukihiro Michiwaki; Goro Nishimura; Haruki Niwa; Yukio Yamada
    International conference proceedings, English
  • Polymeric one- and two-dimensional copper(i) iodide complexes showing photoluminescence tunable by azaaromatic ligands
    Kitada, Nobuo; Ishida, Takayuki
    CrystEngComm, The Royal Society of Chemistry, 16, 2014, Peer-reviwed
    Scientific journal


  • 近赤外発光試薬seMpaiによるがん転移のイメージング
    森屋(齊藤)亮平; 森屋(齊藤)亮平; 森屋(齊藤)亮平; 中山淳; 北田昇雄; 北田昇雄; 牧昌次郎; 牧昌次郎
    2021, 日本癌学会学術総会抄録集(Web), 80th, 202102246186686282
  • Non-invasive detection of pharyngeal residue using near-infrared fluorescence-Evaluation of the detectability by phantom experiments-
    田代剛大; 山田幸生; 丹羽治樹; 牧昌次郎; 牧昌次郎; 北田昇雄; 斉藤亮平; 小池卓二; 小池卓二; 道脇幸博; 西村吾朗
    2020, Optics & Photonics Japan講演予稿集(CD-ROM), 2020, 202102274483914423
  • Evaluation of non-invasive detection for pharyngeal residue in human using near-infrared fluorescence
    田代剛大; 鈴木嵩弘; 山田幸生; 丹羽治樹; 牧昌次郎; 牧昌次郎; 北田昇雄; 齊藤亮平; 小池卓二; 小池卓二; 道脇幸博; 西村吾朗
    2020, 日本機械学会年次大会講演論文集(CD-ROM), 2020, 2424-2667, 202202221715279029
  • Development of a detection system of pharyngeal residue using near-infrared fluorescence for aspiration test
    田代剛大; 鈴木崇弘; 山田幸生; 丹羽治樹; 牧昌次郎; 牧昌次郎; 北田昇雄; 斉藤亮平; 小池卓二; 小池卓二; 道脇幸博; 西村吾朗
    2019, Optics & Photonics Japan講演予稿集(CD-ROM), 2019, 202002289348370842
  • NIR Luciferin Analogues for in vivo Optical Imaging
    北田昇雄; 齊藤亮平; 口丸高弘; 近藤科江; 中山淳; 中山淳; 仙波憲太郎; 牧昌次郎; 牧昌次郎
    2019, 日本癌学会学術総会抄録集(Web), 78th, 202002284518626981
  • ホタルルシフェリンアナログのアリル基導入による波長制御
    北田昇雄; 木山正啓; 齊藤亮平; 斉藤毅; 西山繁; 丹羽治樹; 平野誉; 牧昌次郎
    2017, 日本化学会春季年会講演予稿集(CD-ROM), 97th, 201702284117570298
  • ホタルルシフェリンアナログのアリル基導入による波長制御
    北田昇雄; 木山正啓; 齊藤亮平; 斉藤毅; 西山繁; 丹羽治樹; 平野誉; 牧昌次郎; 牧昌次郎
    2017, 有機電子移動化学討論会講演要旨集, 41st, 201702242781624321
  • 世界最長波長で発光する新規ルシフェリンアナログの創製
    軽部一磨; 北田昇雄; 三浦千弥; 岩野智; 平野誉; 丹羽治樹; 牧昌次郎; 斉藤毅; 西山繁
    2014, 日本化学会講演予稿集, 94th, 4, 0285-7626, 201402247592690770
  • 世界最長波長で発光する新規ホタルルシフェリンアナログの創製
    軽部一磨; 北田昇雄; 三浦千弥; 岩野智; 平野誉; 丹羽治樹; 牧昌次郎; 斉藤毅; 西山繁
    2013, 電気化学秋季大会講演要旨集, 2013, 201302226074753372
  • 世界最長波長で発光する新規ホタルルシフェリンアナログの創製
    軽部一磨; 北田昇雄; 三浦千弥; 岩野智; 平野誉; 丹羽治樹; 牧昌次郎; 斉藤毅; 西山繁
    2013, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 201402217093474830
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