ATSUSHI NAKAMURA
| Department of Engineering Science | Assistant Professor |
| Cluster III (Fundamental Science and Engineering) | Assistant Professor |
| Center for Neuroscience and Biomedical Engineering | Assistant Professor |
Researcher Information
Career
- Apr. 2010
University of Electro-Communications, Research Associate - Apr. 2007
University of Electro-Communications, Research Associate - Apr. 2003
University of Electro-Communications, Research Associate - Apr. 2001 - Mar. 2003
JSPS, Postdoctral fellow researcher - Apr. 2000 - Mar. 2001
CREST, Postdoctral fellow researcher
Educational Background
- Mar. 2000
The University of Tokyo, Graduate School of Science, Department of Biophysics and Biochemistry, Japan - Mar. 1997
The University of Tokyo, Graduate School of Arts and Sciences, Department of Life Sciences, Japan - Mar. 1995
The University of Tokyo, School of Engineering, Department of Chemistry and Biotechnology, Japan - 01 Apr. 1986 - 31 Mar. 1989
兵庫県立尼崎北高等学校, 普通科, Japan
Member History
- Jan. 2024 - Present
庶務幹事、ネットワーク委員, 日本比較生理生化学会, Society - 01 Jan. 2020 - 31 Dec. 2023
日本比較生理生化学会 庶務幹事（事務局）、評議員、ネットワーク委員, Society - 01 Jan. 2020 - 31 Dec. 2023
日本比較生理生化学会評議員, Society - 01 Jan. 2020 - 31 Dec. 2023
日本比較生理生化学会 庶務幹事（事務局）, Society - 01 Apr. 2017 - 31 Mar. 2023
日本時間生物学会評議員, Society - 01 Apr. 2016 - 31 Mar. 2018
日本比較生理生化学会 学会誌編集委員, Society - 01 Apr. 2016 - 31 Mar. 2018
日本味と匂学会評議員, Society - 01 Apr. 2016 - 31 Mar. 2018
日本時間生物学会評議員, Society - 01 Apr. 2015 - 31 Mar. 2017
日本比較生理生化学会評議員, Society
Research Activity Information
Paper
- Enhancement of the Olfactory Response by Lipocalin Cp-Lip1 in Newt Olfactory Receptor Cells
Tadashi Nakamura; Yoshihiro Noumi; Hiroyuki Yamakawa; Atsushi Nakamura; Durige Wen; Xing Li; Xiong Geng; Ken Sawada; Tatsuo Iwasa
Chemical Senses, 40, 523-533, 2019, Peer-reviwed
Scientific journal, English - Effect of sucrose concentration on the impulse amplitudes generated from browfly sugar receptor cells
Nakamura T; Isobe Y; Seto M; Nakamura A; Morita S
The Japanese Journal of Taste and Smell Research, The Japanese association for the study of taste and smell, 21, 265-266, 2014, Peer-reviwed
Scientific journal, Japanese - クロキンバエのフ節D4型味覚毛の各種アミノ酸に対する応答
中村整; 小川絵里; 小川洋史; 栗原隆一郎; 仲村厚志
The Japanese Journal of Taste and Smell Research, The Japanese association for the study of taste and smell, 20, 3, 257-258, 2013, Peer-reviwed
Scientific journal, Japanese - The effect of food intake on expression level of AMPKbeta in the brain of the blowfly, Phormia regina
Atsushi Nakamura; Tadashi Nakamura
Cmparative Physiology and Biochemistry, 28, supplement, 165, Jun. 2011, Peer-reviwed
International conference proceedings, English - Pharmacological analysis of the AMP-activated protein kinase in the regulation of food intake in the blowfly, Phormia regina
Tomoyuki Suzuki; Atsushi Nakamura; Tadashi Nakamura
Cmparative Physiology and Biochemistry, 28, supplement, 165, Jun. 2011, Peer-reviwed
International conference proceedings, English - Contribution of the inositol 1,4,5-trisphosphate transduction cascade to the detection of "bitter" compounds in blowflies
Qin Ouyang; Hiroyasu Sato; Yoshihiro Murata; Atsushi Nakamura; Mamiko Ozaki; Tadashi Nakamura
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, ELSEVIER SCIENCE INC, 153, 3, 309-316, Jul. 2009, Peer-reviwed, Bitter taste detection is very important for many species including flies, because it prevents the ingestion of toxic food. Although it has been known that flies have specific bitter-sensitive taste cells in their contact chemosensilla, the mechanism by which those cells transduce the chemical signal into electrical activity has remained elusive. In this study, we first confirmed that type D4 and D5 tarsal chemosensilla of the blowfly Phormia regina responded well to bitter substances. Then, recording impulses from type D4 chemosensilla, we examined the possibility that a G-protein-coupled inositol 1,4,5-trisphosphate (IP(3))-dependent transduction cascade is of importance in the bitter-sensitive taste cells. We found that the response to bitter substances was depressed by specific inhibitors of G-protein, phospholipase C, or IP(3) receptor in the tarsal taste receptor cells. These results suggest that G-proteins mediate the IP(3) pathway in the transduction cascade in bitter-sensitive receptor cells. (C) 2009 Elsevier Inc. All rights reserved.
Scientific journal, English - IMPLICATION OF MEMBRANE RAFT IN RAT OLFACTORY SIGNAL TRANSDUCTION
Atsushi Nakamura; Hiroyuki Takeda; Tadashi Nakamura
JOURNAL OF PHYSIOLOGICAL SCIENCES, SPRINGER TOKYO, 59, S1, 148-148, 2009, Peer-reviwed
Scientific journal, English - Odour of limonene affects feeding behaviour in the blowfly, Phormia regina
Atsushi Nakamura; Tomoyuki Suzuki; Daiki Taniguchi; Atsushi Matsuda; Manabu Tobeta; Tadashi Nakamura
NEUROSCIENCE LETTERS, ELSEVIER IRELAND LTD, 446, 1, 36-39, Nov. 2008, Peer-reviwed, Experiences of odours at meals are thought to affect food preference in many animals. To study appetite modulation by odours, we established a new experimental system based on the modification of a previous method, where flies fed sucrose flavoured with D-limonene subsequently showed reduced appetite to plain sucrose. In this new experimental system, a fly population was divided into two groups: (1) the "simultaneous" group of flies was exposed to D-limonene and sucrose simultaneously for 10 min, and (2) the "separate" group of flies was exposed to sucrose alone for 10 min, and 1 h later, to limonene for 10 min. The appetite of flies in the "separate" group for sucrose was unaffected by the experiment, but the appetite of flies in the "simultaneous" group was significantly decreased, and this effect lasted for >= 3 days. To investigate if this appetite modulation by D-limonene was based on long-term memory formation (protein synthesis), we examined the effects of the protein synthesis inhibitor, cycloheximide. Injection of cycloheximide 1 h after exposure to limonene and sucrose inhibited the appetite suppression in the "simultaneous" group of flies. In addition, to exclude the possibility that D-limonene exerted its effect through taste, rather than odour, we examined the effect of removing of the olfactory organs, antennae and maxillary palps on appetite modulation by D-limonene. When the olfactory organs were removed, no reduction in appetite was observed in the flies in the "simultaneous" group, indicating olfaction. In our new and effective appetite-modulation system for flies, modulation of appetite by olfactory detection of D-limonene was shown. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Scientific journal, English - Two isoforms of chicken melanopsins show blue light sensitivity
Masaki Torii; Daisuke Kojima; Toshiyuki Okano; Atsushi Nakamura; Akihisa Terakita; Yoshinori Shichida; Akimori Wada; Yoshitaka Fukada
FEBS LETTERS, WILEY, 581, 27, 5327-5331, Nov. 2007, Peer-reviwed, Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Scientific journal, English - Implication of membrane raft in rat olfactory signal transduction
Atsushi Nakamura; Hiroyuki Takeda; Tadashi Nakamura
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, ELSEVIER SCIENCE INC, 142, 4, 481-481, Dec. 2005
Scientific journal, English - Regulatory mechanism for the stability of the meta II intermediate of pinopsin
A Nakamura; D Kojima; T Okano; H Imai; A Terakita; Y Shichida; Y Fukada
JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, 129, 2, 329-334, Feb. 2001, Peer-reviwed, Pinopsin is a chicken pineal photoreceptive molecule with a possible role in photoentrainment of the circadian clock. Sequence comparison among members of the rhodopsin family has suggested that pinopsin might have properties more similar to cone visual pigments than to rhodopsin, but the lifetime of the physiologically active intermediate (meta II) of pinopsin is rather similar to that of metarhodopsin II, which is far more stable than meta II intermediates of cone visual pigments [Nakamura, A. et al., (1999) Biochemistry 38, 14738-14745]. In the present study, we investigated the amino acid residue(s) contributing to this unique property of pinopsin by using site-directed mutagenesis to pinopsin-specific structural features, (i) Ser171, (ii) Asn184, and (iii) the second extracellular loop two-amino acids shorter than that of cone visual pigments. The meta II stability of the 171/184 double mutant of pinopsin (S171R/N184D) is almost the same as that of wild-type pinopsin. In contrast, the meta II lifetime is markedly shortened (one third) by introduction of the third mutation (replacement of a six-amino acid stretch, 188-193, by the corresponding eight residues of chicken green-sensitive cone pigment) to the 171/184 double mutant of pinopsin. Consistently, meta II of the green-sensitive pigment mutant, in which the eight-amino acid stretch is inversely replaced by the corresponding six residues of pinopsin, is more stable than meta II of the wild-type pigment. These results strongly suggest that the specific sequence and/or the number of residues at amino acids 188-193 in pinopsin play an important role in the stabilization of the meta II intermediate.
Scientific journal, English - Chimeric nature of pinopsin between rod and cone visual pigments
A Nakamura; D Kojima; H Imai; A Terakita; T Okano; Y Shichida; Y Fukada
BIOCHEMISTRY, AMER CHEMICAL SOC, 38, 45, 14738-14745, Nov. 1999, Peer-reviwed, Chicken pineal pinopsin is the first example of extra-retinal opsins, but little is known about its molecular properties as compared with retinal rod and cone opsins. For characterization of extraretinal photon signaling, we have developed an overexpression system providing a sufficient amount of purified pinopsin. The recombinant pinopsin, together with similarly prepared chicken rhodopsin and green-sensitive cone pigment, was subjected to photochemical and biochemical analyses by using low-temperature spectroscopy and the transducin activation assay. At liquid nitrogen temperature (-196 degrees C), we detected two kinds of photoproducts, bathopinopsin and isopinopsin, having their absorption maxima (lambda(max)) at 527 and similar to 440 nm, respectively, and we observed complete photoreversibility among pinopsin, bathopinopsin, and isopinopsin. A close parallel of the photoreversibility to the rhodopsin system strongly suggests that light absorbed by pinopsin triggers the initial event of cis-trans isomerization of the 11-cis-retinylidene chromophore. Upon warming, bathopinopsin decayed through a series of photobleaching intermediates: lumipinopsin (lambda(max) 461 nm), metapinopsin I (460 nm), metapinopsin II (385 nm), and metapinopsin III (460 nm). Biochemical and kinetic analyses showed that metapinopsin II is a physiologically important photoproduct activating transducin. Detailed kinetic analyses revealed that the formation of metapinopsin IT is as fast as that of a chicken cone pigment, green, but that the decay process of metapinopsin II is as slow as that of the rod pigment, rhodopsin. These results indicate that pinopsin is a new type of pigment with a chimeric nature between rod and cone visual pigments in terms of the thermal behaviors of the meta II intermediate. Such a long-lived active state of pinopsin may play a role in the pineal-specific phototransduction process.
Scientific journal, English - Immunocytochemical identification of pinopsin in pineal glands of chicken and pigeon
T Okano; Y Takanaka; A Nakamura; K Hirunagi; A Adachi; S Ebihara; Y Fukada
MOLECULAR BRAIN RESEARCH, ELSEVIER SCIENCE BV, 50, 1-2, 190-196, Oct. 1997, Peer-reviwed, Pinopsin is a blue-sensitive photoreceptive molecule possibly involved in photic entrainment of the circadian pacemaker in the chicken pineal gland. To characterize pinopsin as a circadian photoreceptor, antibodies were raised against the C-terminal portion of pinopsin. Its expected from the divergence of the amino acid sequence of this region, the resultant antibody cross-reacted with neither chicken rhodopsin nor red-sensitive cone pigment (chicken red). in Western blot analysis, the antibody stained a single band of 42-kDa protein in a detergent-extract of chicken pineal membranes, suggesting that pinopsin (calculated molecular weight, 38 187) might be glycosylated and/or palmitoylated. Immunocytochemical examination of pineal sections of the chicken and the pigeon with this antibody revealed strong positive images for most of the membrane structures in the lumen of the follicles. This antibody also stained string- and bulb-shaped structures of the chicken parafollicular cells, the morphology of which resembles those of retinal photoreceptor cells. In contrast to the predominant distribution of pinopsin, a monoclonal antibody specific for chicken red stained a smaller number of membrane structures in the lumen of chicken pineal follicles. These results strongly suggest that the chicken pined gland contains at least two types of photoreceptive molecules, pinopsin (major) and chicken red (minor). We show that the former molecule is localized in parafollicular pinealocytes and in the outer segments of pinealocytes that make contact with the follicular lumen. (C) 1997 Elsevier Science B.V.
Scientific journal, English
MISC
- ホタル生物発光の人工発光系へのモデル化とヒトの生体内精密計測に向けた挑戦
仲村厚志; 牧昌次郎
日本農芸化学会, Feb. 2022, 化学と生物, 60, 2, 72-78, Japanese, Peer-reviwed, Introduction scientific journal - P-032 Effect of the sucrose concentration on the impulse amplitude generated from the sugar receptor cell in the blowfly
Nakamura Tadashi; Isobe Youhei; Seto Mihoshi; Nakamura Atsushi; Morita Shuji
The Japanese Association for the Study of Taste and Smell, Dec. 2014, The Japanese journal of taste and smell research, 21, 3, 265-266, Japanese, 1340-4806, 110009892282, AA11426393 - ショウジョウバエの味覚受容メカニズム
仲村厚志; 中村整
2007, バイオメカニズム学会誌, 31, 3, 138-142, Japanese, Introduction other
Lectures, oral presentations, etc.
- ルシフェリン誘導体TokeOniによる 新規生物発光現象
仲村 厚志; 林 唯奈; 猪狩 侑真; 北田 昇雄; 森屋 亮平; 牧 昌次郎; 吉川 朋子
Invited oral presentation, Japanese, 生物発光化学発光研究会 第38回学術講演会, Invited, Peer-reviewed
11 Nov. 2023
11 Nov. 2023- 11 Nov. 2023 - Implication of CYP for new bioluminescence system by luciferin analogue TokeOni
Atsushi Nakamura; Yuna Hayashi; Yukimasa Ikari; Nobuo Kitada; Ryohei SAITO-MORIYA; Shojiro Maki; Tomoko Yoshikawa
Oral presentation, English, 第50回 日本毒性学会学術年会, Peer-reviewed
20 Jun. 2023
19 Jun. 2023- 21 Jun. 2023 - Bioluminescence by luciferin analogues in mice, pill bugs, and blow flies
Yukimasa Ikari; Yuna Hayashi; Mifuru Fukuda; Nobuo Kitada; Ryohei Saito-Moriya; Shojiro Maki; Tomoko yoshikawa; Atsushi Nakamura
Poster presentation, English, 日本比較生理生化学第44回大会
Nov. 2022 - Implication of CYP for bioluminescence by luciferin analogue TokeOni in mice, pill bugs, and blow flies
Yuna Hayashi; Mifuru Fukuda; Nobuo Kitada; Ryohei Saito; Shojiro Maki; Tomoko yoshikawa; Atsushi Nakamura
Poster presentation, English, 第49回 日本毒性学会学術年会
Jul. 2022 - Bioluminescence by luciferin analogues in mice, pill bugs, and blow flies
Yuna Hayashi; Mifuru Fukuda; Nobuo Kitada; Ryohei Saito; Shojiro Maki; Tomoko yoshikawa; Atsushi Nakamura
Oral presentation, English, The 43rd Online Conference of the Japanese Society for Comparative Physiology and Biochemistry in Sapporo, 日本比較生理生化学会, オンライン（北海道大学）, Domestic conference
05 Dec. 2021 - 匂い刺激を用いたマウス肝臓概日時計の位相シフト
島村瑞穂; 佐藤慎之助; 吉川朋子; 仲村厚志
Oral presentation, Japanese, 第28回日本時間生物学会学術大会, 日本時間生物学会, 沖縄, Domestic conference
20 Nov. 2021 - Bioluminescence by luciferin analogues in mammals and insects
Yuna Hayashi; Shojiro Maki; Ryohei Saito; Nobuo Kitada; Tomoko yoshikawa; Atsushi Nakamura
Oral presentation, English, 日本比較生理生化学会第42回大会, 日本比較生理生化学会, 山形大学, Domestic conference
23 Nov. 2020 - Enzymes of mammalian origin catalyze bioluminescence of mouse liver
Yuna Hayashi; Shojiro Maki; Ryohei Saito; Nobuo Kitada; Tomoko yoshikawa; Atsushi Nakamura
Poster presentation, Japanese, The 91th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, オンライン, Domestic conference
04 Sep. 2020 - ホタルルシフェリン誘導体を投与したマウスにみられる肝臓発光
Yuna Hayashi; Ryohei Saito; Shojiro Maki; Tomoko yoshikawa; Atsushi Nakamura
Oral presentation, Japanese, The 90th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, osaka, Domestic conference
12 Sep. 2019 - マウス視交叉上核における免疫染色法を用いたAMPキナーゼの解析
Kenji ishida; Naoto Takise; Kouki Hoshito; Hiroaki Maeda; Tomoko Yoshikawa; Atsushi Nakamura
Oral presentation, Japanese, The 90th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, osaka, Domestic conference
12 Sep. 2019 - ホタルルシフェリン誘導体によるマウス肝臓の発光
仲村 厚志; 林 唯奈; 斎藤 亮平; 牧 昌次郎; 吉川 朋子
Poster presentation, Japanese, 第４１回日本分子生物学会年会, 日本分子生物学会, 横浜, 生物発光反応における化学反応は、ルシフェリン‐ルシフェラーゼ反応として知られている。ホタル生物発光は黄緑色（560nm程度）の発光波長をもつ。しかし、この波長帯ではin vivoイメージングを行う際に、水、ヘモグロビンによる吸収を受けやすい。吸収されにくい波長帯での発光を求めて、ホタル生物発光反応におけるルシフェリン誘導体が多数有機合成され、アカルミネ®（和光純薬工）とTokeOni（SIGMA-ARDRICH）が市販化されている。通常、in vivoイメージングでは、ホタル発光酵素が遺伝子導入された生体に基質を投与することで、基質と酵素を生体内で反応させて発光させる。しかし、ホタル由来の遺伝子を導入していない野生型マウスに『アカルミネ®』や『TokeOni』を投与した際、肝臓で原因不明の発光が観察されることがある。我々は肝臓発光のメカニズムを解明すべく、発光へと導く肝臓内在性酵素の特定および、発光条件の特定に取り組んでいる。, Domestic conference
29 Nov. 2018 - The influence of the odor stimulation on the circadian clock in mouse liver
Shinnosuke SATO; Miho SUZUKI; Tomoko YOSHIKAWA; Atsushi NAKAMURA
Poster presentation, English, 日本比較生理生化学会 第40回神戸大会, 日本比較生理生化学会, Kobe, Domestic conference
23 Nov. 2018 - Diurnal variation of AMPK activity in the blowfly, Phormia regina
Atsushi NAKAMURA
Poster presentation, English, 日本比較生理生化学会 第40回神戸大会, 日本比較生理生化学会, Kobe, Domestic conference
23 Nov. 2018 - Bioluminescence of mouse liver generated by a firefly luciferin analogue
Atsushi Nakamura
Nominated symposium, Japanese, The 89th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, Sapporo, 生体光イメージング技術は、ライフサイエンス分野、特にがんや脳機能研究において必要不可欠であり、中でもホタル生物発光系は最も汎用されている。牧らによって開発されたホタルルシフェリン誘導体、アカルミネ及びトケオニは、ヘモグロビンなどの吸収を受けにくい近赤外領域に発光ピークをもつため、これまで生体深部のin vivoイメージング実験で成果をあげている。一方で、これらの試薬をマウスに投与すると、ホタルルシフェラーゼ遺伝子を導入していないにもかかわらず肝臓に発光が検出される現象が観察されている。本研究では、この現象の解明を目指し、まず肝臓発光量が増減する条件を検討した。雌雄の別や、計測する時間帯、あるいは週令などの違いによって、発光量が変化する可能性を調べた。さらに最終的に、この現象に関わる分子を同定することを目指し、in vitroの条件下で発光する条件の探索を行った。, Domestic conference
15 Sep. 2018 - Bioluminescence of mouse liver administered firefly luciferin analogue
Yuna Hayashi; Ryohei Saito; Shojiro Maki; Tomoko yoshikawa; Atsushi Nakamura
Poster presentation, Japanese, The 89th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, Sapporo, 生物発光反応における化学反応は、ルシフェリン‐ルシフェラーゼ反応として知られている。ホタル生物発光は黄緑色（560nm程度）の発光波長をもつ。しかし、この波長帯ではin vivoイメージングを行う際に、水、ヘモグロビンによる吸収を受けやすい。吸収されにくい波長帯での発光を求めて、ホタル生物発光反応におけるルシフェリン誘導体が多数有機合成され、アカルミネ®（和光純薬工）とTokeOni（SIGMA-ARDRICH）が市販化されている。通常、in vivoイメージングでは、ホタル発光酵素が遺伝子導入された生体に基質を投与することで、基質と酵素を生体内で反応させて発光させる。しかし、ホタル由来の遺伝子を導入していない野生型マウスに『アカルミネ®』や『TokeOni』を投与した際、肝臓で原因不明の発光が観察されることがある。我々は肝臓発光のメカニズムを解明すべく、発光へと導く肝臓内在性酵素の特定および、発光条件の特定に取り組んでいる。, Domestic conference
13 Sep. 2018 - The influence of the odor stimulation on the circadian clock in the liver
Shinnosuke Sato; Miho Suzuki; Tomoko Yoshikawa; Atsushi Nakamura
Poster presentation, Japanese, The 89th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, Sapporo, 哺乳類の概日システムは、脳の視交叉上核（SCN）に存在する中枢時計と、末梢組織に存在する末梢時計からなる。中枢時計は光情報に短期間で同調するが、末梢時計は同調するためにより多くの日数を要することが知られている。このため、時差のある地域への海外旅行や交代勤務などが、中枢時計と末梢時計からなる概日システムを乱し概日時計間の位相のずれを引き起こす。ヒトやマウスを用いた研究で、概日時計と外界環境との位相のずれが、がんや糖尿病の原因の一つである可能性が指摘されている。本研究では末梢時計の調節を目的とし、匂い刺激が概日時計に及ぼす影響を調べた。匂い刺激や光サイクルの変化による概日時計の位相変化を測定するため、発光レポーターを用い、培養組織の時計遺伝子発現リズムの計測を行った。LD12 : 12の明暗サイクル またはこの光周期を6時間前進させたサイクル下においたPer1-lucマウスについて匂い刺激が概日時計に与える影響を調べた。, Domestic conference
13 Sep. 2018 - マウス視交叉上核における AMPキナーゼの免疫組織化学的解析
Kenji ishida; Naoto Takise; Kouki Hoshito; Hiroaki Maeda; Tomoko Yoshikawa; Atsushi Nakamura
Poster presentation, Japanese, The 89th Annual Meeting of the Zoological Society of Japan, The Zoological Society of Japan, Sapporo, 地球上のあらゆる生物には、約24時間周期のリズムを刻む概日時計が備わっている。個体のリズム形成に重要な役割を持つ時計は中枢時計と呼ばれ、哺乳類では視交叉上核（SCN）に存在する。中枢時計は全身に存在する末梢時計のリズムを調節している。AMP活性化プロテインキナーゼは体内のエネルギー状態を継続的に保つ役割を担う蛋白質リン酸化酵素である。AMPKは末梢時計において、細胞内のエネルギー状態の変化を受けてリズム同調を行うと報告されている。一方、AMPK のSCNでの役割に関しては不明である。そこで、AMPKの活性化を検出する抗体を用いた免疫組織化学的解析を行った。
AMPK基質蛋白質に対する抗体を用いた解析から、SCNでのAMPKによる基質蛋白質リン酸化が昼の時間帯に低く、夜の時間帯に高い活性を持つことが明らかになった。また夜の前半に光照射を行った場合、AMPKによるリン酸化が低下していることが確認された。, Domestic conference
13 Sep. 2018 - 嗅神経特異的Gタンパク質Golfの細胞膜ラフトへの局在
仲村厚志
Public symposium, Japanese, 第2回 先端味覚研究セミナー, 日本味と匂学会, 東京
Aug. 2012
Courses
- 化学生命工学実験第二
Present
The University of Electro-Communications - 化学生命工学実験第一
Present
The University of Electro-Communications - 生体機能システム実験第二
The University of Electro-Communications - 生体機能システム実験第一
The University of Electro-Communications - 化学生命工学実験第二
The University of Electro-Communications - 生体機能システム実験第二
The University of Electro-Communications - 生体機能システム実験第二
電気通信大学 - 化学生命工学実験第一
電気通信大学 - 生体機能システム実験第一
The University of Electro-Communications - 生体機能システム実験第一
電気通信大学
Affiliated academic society
Research Themes
- 新規発光技術による診断システムの創製
仲村 厚志
日本医療研究開発機構（ＡＭＥＤ）, 研究助成
01 Aug. 2022 - 31 Mar. 2023 - 哺乳類初の発光酵素が開く新規診断方法の基盤技術開発
仲村 厚志
科学技術振興機構, 研究助成, Principal investigator
01 May 2021 - 31 Mar. 2022 - 哺乳類初の発光酵素の探索-新しい診断技術の創生を目指して
仲村 厚志
科学技術振興機構, 研究助成, Principal investigator
02 Sep. 2019 - 31 Aug. 2020 - Analyses of physiological role of circadian clock in the ovary
NAKAMURA Tomoko; NAKAMURA Atsushi
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Hokkaido University, Grant-in-Aid for Scientific Research (C), Current understanding in the field is that the circadian clock in the suprachiasmatic nucleus controls the phase of circadian clock in the peripheral organs. However, the mechanism of the control is remained to be shown. In this study, we used the rat ovary as a model of the peripheral organ, and investigated the potency of LH to control the phase of the circadian clock. When LH level was kept at a constant level in the rat by a pharmacological or surgical method, the amplitude of circadian oscillation was significantly decreased. The result indicates that LH is important to maintain circadian oscillation in the ovary., 19599001
2007 - 2009