Daisuke HOSHINO
| Department of Engineering Science | Associate Professor |
| Cluster III (Fundamental Science and Engineering) | Associate Professor |
| Center for Neuroscience and Biomedical Engineering | Associate Professor |
| Division of General Education（School of Informatics and Engineering ） | Associate Professor |
| Division of General Education（Graduate School of Informatics and Engineering） | Associate Professor |
Researcher Information
Career
- Oct. 2017 - Present
The University of Electro-Communications, School of Informatics and Engineering, 准教授 - Oct. 2017 - Present
The University of Electro-Communications, School of Informatics and Engineering, Division of General Education, 准教授 - Apr. 2017 - Sep. 2017
The University of Tokyo, Department of Biological Sciences, 特任助教 - Apr. 2014 - Mar. 2017
The University of Tokyo, Graduate School of Science, Department of Biological Sciences, 日本学術振興会 特別研究員PD - Apr. 2011 - Mar. 2014
The University of Tokyo, 大学院・総合文化研究科, 助教
Research Activity Information
Paper
- Cooling-induced changes in intracellular hydrogen peroxide and gene expression in mouse skeletal muscle in vivo.
Ryotaro Kano; Reo Takeda; Yuta Sotani; Ryo Takagi; Ayaka Tabuchi; Hideki Shirakawa; David C Poole; Yutaka Kano; Daisuke Hoshino
American journal of physiology. Regulatory, integrative and comparative physiology, 07 May 2025, Peer-reviwed, True, Changes in intracellular hydrogen peroxide concentration ([H2O2]) constitute an important signal controlling cellular adaptations. In response to cooling, decreases in [H2O2] and changes in antioxidant-related gene expression have been observed in skeletal muscle. However, the specific temperature dependence of cooling-induced [H2O2] changes and their quantitative relationship to induced gene expression are unknown. This investigation tested the hypothesis that differences in muscle cytosolic and mitochondrial [H2O2] changes during cooling/rewarming determine the pattern of H2O2-related gene expression. H2O2-sensitive cytosolic (HyPer7) and mitochondrial (MLS-HyPer7) fluorescent proteins were expressed into tibialis anterior (TA) muscle of male C57BL/6J mice. The temperature dependence of [H2O2] was determined via in vivo imaging during 3-minute cooling protocol from 35°C to 0°C. Two cooling patterns (6 bouts of intermittent cooling, I-Cool vs. sustained cooling, S-Cool; both to 13°C) were applied over 60 min. Three hours after cooling, the muscles were removed and gene expression was evaluated using real-time PCR. The decrease in [H2O2] was observed in both cytosolic and mitochondrial compartments from 35°C to 13°C, but was of greater magnitude in the cytosol; in contrast, further cooling from 12°C to 0°C induced a rebound increase especially in cytosolic [H2O2]. I-Cool increased the mRNA level of Nrf2 (+15%, p < 0.001). S-Cool decreased the mRNA levels of Sod2, Cat, and Ucp3 (i.e., -20%, -23%, and -30%, respectively, p<0.05). In conclusion, the greatest decrease in temperature-dependent [H2O2] occurred at 13°C in the cytosolic and mitochondrial compartments of muscle fibers and I-Cool increased Nrf2 mRNA expression, while S-Cool decreased several antioxidant-related genes.
Scientific journal, English - In vivo intracellular Ca2+ profiles after eccentric rat muscle contractions: addressing the mechanistic bases for repeated bout protection.
Ayaka Tabuchi; Yudai Kikuchi; Ryo Takagi; Yoshinori Tanaka; Daisuke Hoshino; David C Poole; Yutaka Kano
Journal of applied physiology (Bethesda, Md. : 1985), 138, 1, 1-12, 01 Jan. 2025, Peer-reviwed, True, Eccentric contractions (ECC) are accompanied by accumulation of intracellular calcium ions ([Ca2+]i) and induce skeletal muscle damage. Suppressed muscle damage in repeated bouts of ECC is well characterized, however, whether it is mediated by altered Ca2+ profiles remains unknown. PURPOSE: We tested the hypothesis that repeated ECC suppresses Ca2+ accumulation via adaptions in Ca2+ regulation. METHODS: Male Wistar rats were divided into two groups: ECC single bout (ECC-SB) and repeated bout (ECC-RB). Tibialis anterior (TA) muscles were subjected to ECC (40 times, 5 sets) once (ECC-SB), or twice 14 days apart (ECC-RB). Under anesthesia, the TA muscle was loaded with Ca2+ indicator Fura-2 AM and the 340/380 nm ratio was evaluated as [Ca2+]i. Ca2+ handling proteins were measured by western blots. RESULTS: ECC induced [Ca2+]i increase in both groups, but ECC-RB evinced a markedly suppressed [Ca2+]i (Time: P < 0.01, Group: P = 0.0357). 5 hours post-ECC, in contrast to the localized [Ca2+]i accumulation in ECC-SB, ECC-RB exhibited lower and more uniform [Ca2+]i (P < 0.01). In ECC-RB mitochondria Ca2+ uniporter complex components, MCU and MICU2, were significantly increased pre-second ECC bout (P < 0.01) and both SERCA1 and MICU1 were better preserved after contractions (P < 0.01). CONCLUSION: 14 days after novel ECC skeletal muscle mitochondrial Ca2+ regulating proteins were elevated. Following subsequent ECC [Ca2+]i accumulation and muscle damage were suppressed and SERCA1 and MICU1 preserved. These findings suggest that tolerance to a subsequent ECC bout is driven, at least in part, by enhanced mitochondrial and SR Ca2+ regulation.
Scientific journal, English - Time-series transcriptomics reveals distinctive mRNA expression dynamics associated with gene ontology specificity and protein expression in skeletal muscle after electrical stimulation-induced resistance exercise.
Tatsuya Kusano; Yuta Sotani; Reo Takeda; Atsushi Hatano; Kentaro Kawata; Ryotaro Kano; Masaki Matsumoto; Yutaka Kano; Daisuke Hoshino
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, 22, e70153, 30 Nov. 2024, Peer-reviwed, True, Resistance exercise upregulates and downregulates the expression of a wide range of genes in skeletal muscle. However, detailed analysis of mRNA dynamics such as response rates and temporal patterns of the transcriptome after resistance exercise has not been performed. We aimed to clarify the dynamics of time-series transcriptomics after resistance exercise. We used electrical stimulation-induced muscle contraction as a resistance exercise model (5 sets × 10 times of 3 s of 100-Hz electrical stimulation) on the tibialis anterior muscle of rats and measured the transcriptome in the muscle before and at 0, 1, 3, 6, and 12 h after muscle contractions by RNA sequencing. We also examined the relationship between the parameters of mRNA dynamics and the increase in protein expression at 12 h after muscle contractions. We found that the function of the upregulated genes differed after muscle contractions depending on their response rate. Genes related to muscle differentiation and response to mechanical stimulus were enriched in the sustainedly upregulated genes. Furthermore, there was a positive correlation between the magnitude of upregulated mRNA expression and the corresponding protein expression level at 12 h after muscle contractions. Although it has been theoretically suggested, this study experimentally demonstrated that the magnitude of the mRNA response after electrical stimulation-induced resistance exercise contributes to skeletal muscle adaptation via increases in protein expression. These findings suggest that mRNA expression dynamics such as response rate, a sustained upregulated expression pattern, and the magnitude of the response contribute to mechanisms underlying adaptation to resistance exercise.
Scientific journal, English - Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice
Ryotaro Kano; Tatsuya Kusano; Reo Takeda; Hideki Shirakawa; David C. Poole; Yutaka Kano; Daisuke Hoshino
Journal of Applied Physiology, American Physiological Society, 137, 3, 778-788, 01 Sep. 2024, Peer-reviwed, This in vivo model successfully characterized the effects of eccentric (ECC) and concentric (CONC) contractions on cytosolic and mitochondrial [H2O2] in mouse skeletal muscle. Compared with CONC, ECC induced higher and more sustained [H2O2]cyto—an effect that was abolished by Nox2 inhibition. ECC-induced [H2O2]cyto elevations were requisite for altered gene expression.
Scientific journal - Cmah deficiency blunts cellular senescence in adipose tissues and improves whole-body glucose metabolism in aged mice.
Reo Takeda; Ayaka Tabuchi; Yudai Nonaka; Ryotaro Kano; Mizuki Sudo; Yutaka Kano; Daisuke Hoshino
Geriatrics & gerontology international, 15 Nov. 2023, Peer-reviwed, False, AIM: Cytidine monophosphate-N-acetylneuraminic acid (Neu5Ac) hydroxylase (Cmah) is an enzyme, which converts Neu5Ac to the sialic acid Neu5Gc. Neu5Gc is thought to increase inflammatory cytokines, which are, in part, produced in senescent cells of adipose tissues. Cellular senescence in adipose tissues induces whole-body aging and impaired glucose metabolism. Therefore, we hypothesized that Cmah deficiency would prevent cellular senescence in adipose tissues and impaired glucose metabolism. METHODS: Wild-type (WT) and Cmah knockout (KO) mice aged 24-25 months were used. Whole-body metabolism was assessed using a metabolic gas analysis system. We measured blood glucose and insulin concentrations after oral glucose administration. The size of the lipid droplets in the liver was quantified. Markers of cellular senescence and senescence-associated secretory phenotypes were measured in adipose tissues. RESULTS: Cmah KO had significantly increased VO2 and energy expenditure (P < 0.01). Unlike glucose, the insulin concentration after oral glucose administration was significantly lower in the Cmah KO group than in the WT group (P < 0.001). Lipid droplets in the liver were significantly lower in the Cmah KO group than in the WT group (P < 0.05). The markers of cellular senescence and senescence-associated secretory phenotypes in the adipose tissues were significantly lower in the Cmah KO group than in the WT group (P < 0.05). CONCLUSIONS: Cmah deficiency blunted cellular senescence in adipose tissues and improved whole-body glucose metabolism. These characteristics in aged Cmah KO mice might be associated with higher energy expenditure. Geriatr Gerontol Int 2023; ••: ••-••.
Scientific journal, English - Cooling of male rat skeletal muscle during endurance-like contraction attenuates contraction-induced PGC-1α mRNA expression.
Daisuke Hoshino; Ryota Wada; Yutaro Mori; Reo Takeda; Yudai Nonaka; Ryotaro Kano; Ryo Takagi; Yutaka Kano
Physiological reports, 11, 21, e15867, Nov. 2023, Peer-reviwed, True, This study aimed to determine effects of cooling on contraction-induced peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and vascular endothelial growth factor (VEGF) gene expression, phosphorylations of its related protein kinases, and metabolic responses. Male rats were separated into two groups; room temperature (RT) or ice-treated (COLD) on the right tibialis anterior (TA). The TA was contracted isometrically using nerve electrical stimulation (1-s stimulation × 30 contractions, with 1-s intervals, for 10 sets with 1-min intervals). The TA was treated before the contraction and during 1-min intervals with an ice pack for the COLD group and a water pack at RT for the RT group. The muscle temperature of the COLD group decreased to 19.42 ± 0.44°C (p < 0.0001, -36.4%) compared with the RT group after the experimental protocol. An increase in mRNA expression level of PGC-1α, not VEGF, after muscle contractions was significantly lower in the COLD group than in the RT group (p < 0.0001, -63.0%). An increase in phosphorylated AMP-activated kinase (AMPK) (p = 0.0037, -28.8%) and a decrease in glycogen concentration (p = 0.0231, +106.3%) after muscle contraction were also significantly inhibited by cooling. Collectively, muscle cooling attenuated the post-contraction increases in PGC-1α mRNA expression coinciding with decreases in AMPK phosphorylation and glycogen degradation.
Scientific journal, English - In vivo cytosolic H2O2 changes and Ca2+ homeostasis in mouse skeletal muscle.
Ryotaro Kano; Ayaka Tabuchi; Yoshinori Tanaka; Hideki Shirakawa; Daisuke Hoshino; David C Poole; Yutaka Kano
American journal of physiology. Regulatory, integrative and comparative physiology, 30 Oct. 2023, Peer-reviwed, True, Hydrogen peroxide (H2O2) and calcium ions (Ca2+) are functional regulators of skeletal muscle contraction and metabolism. Although H2O2 is one of the activators of the type-1 ryanodine receptor (RyR1) in the Ca2+ release channel, the interdependence between H2O2 and Ca2+ dynamics remains unclear. This study tested the following hypotheses using an in vivo model of mouse tibialis anterior (TA) skeletal muscle. 1. Under resting conditions, elevated cytosolic H2O2 concentration ([H2O2]cyto) leads to a concentration-dependent increase in cytosolic Ca2+concentration ([Ca2+]cyto) through its effect on RyR1. 2. In hypoxia (cardiac arrest) and muscle contractions (electrical stimulation), increased [H2O2]cyto induce Ca2+ accumulation. Cytosolic H2O2 (HyPer7) and Ca2+ (Fura-2) dynamics were resolved by TA bioimaging in C57BL/6J male mice under four conditions: Elevated exogenous H2O2, Cardiac arrest, Twitch and Tetanic contractions. Exogenous H2O2 (0.1-100mM) induced a concentration-dependent increase in [H2O2]cyto (+55%,0.1mM; +280%,100mM) and an increase in [Ca2+]cyto (+3%,1.0mM; +8%,10mM). This increase in [Ca2+]cyto was inhibited by pharmacological inhibition of RyR1 by dantrolene. Cardiac arrest-induced hypoxia increased [H2O2]cyto (+33%) and [Ca2+]cyto (+20%) 50min post-cardiac arrest. Compared to exogenous 1.0mM H2O2 condition, [H2O2]cyto after tetanic contractions rose less than one-tenth as much, while [Ca2+]cyto was 4.7-fold higher. In conclusion, substantial increases in [H2O2]cyto levels evoke only modest Ca2+ accumulation via their effect on the sarcoplasmic reticulum RyR1. On the other hand, contrary to hypoxia secondary to cardiac arrest, increases in [H2O2]cyto from contractions are small, indicating that H2O2 generation is unlikely to be a primary factor driving the significant Ca2+ accumulation after, especially tetanic, muscle contractions.
Scientific journal, English - Chronic repetitive cooling and caffeine-induced intracellular Ca²⁺ elevation differentially impact adaptations in slow- and fast-twitch rat skeletal muscles
Ryo Takagi; Ayaka Tabuchi; Kosei Hayakawa; Shion Osana; Hiroya Yabuta; Daisuke Hoshino; David C. Poole; Yutaka Kano
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, American Physiological Society, 19 Jun. 2023, Peer-reviwed, Intracellular Ca²⁺ concentration ([Ca²⁺]i) is considered important in the regulation of skeletal muscle mass. This study tested the hypothesis that chronic repeated cooling and/or caffeine ingestion would acutely increase [Ca²⁺]i and hypertrophy muscles potentially in a fiber type-dependent manner. Control rats and those fed caffeine, were subjected to repeated bidiurnal treatments of percutaneous icing, under anaesthesia, to reduce the muscle temperature below ~5°C. The predominantly fast twitch tibialis anterior (TA) and slow twitch soleus (SOL) muscles were evaluated after 28 days intervention. The [Ca²⁺]i elevating response to icing was enhanced by caffeine loading only in the SOL muscle, with the response present across a significantly higher temperature range than in the TA muscle under caffeine loading conditions. In both the TA and SOL muscles, myofiber cross-sectional area (CSA) was decreased by chronic caffeine treatment (mean reductions of 10.5% and 20.4%, respectively). However, in the TA , but not the SOL, CSA was restored by icing (+15.4 ± 4.3% vs. non-iced, P<0.01). In the SOL but not TA, icing+caffeine increased myofiber number (20.5 ± 6.7%, P<0.05) and satellite cell density (2.5 ± 0.3-fold) in cross sections. These contrasting muscle responses to cooling and caffeine may reflect fiber-type specific [Ca²⁺]i responses and/or differential responses to elevated [Ca²⁺]i.
Scientific journal - Branched-chain amino acid supplementation suppresses the detraining-induced reduction of mitochondrial content in mouse skeletal muscle.
Yutaka Matsunaga; Yuki Tamura; Kenya Takahashi; Yu Kitaoka; Yumiko Takahashi; Daisuke Hoshino; Tomoyasu Kadoguchi; Hideo Hatta
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, 12, e22628, Dec. 2022, Peer-reviwed, True, Exercise training enhances oxidative capacity whereas detraining reduces mitochondrial content in skeletal muscle. The strategy to suppress the detraining-induced reduction of mitochondrial content has not been fully elucidated. As previous studies reported that branched-chain amino acid (BCAA) ingestion increased mitochondrial content in skeletal muscle, we evaluated whether BCAA supplementation could suppress the detraining-induced reduction of mitochondrial content. Six-week-old male Institute of Cancer Research (ICR) mice were randomly divided into four groups as follows: control (Con), endurance training (Tr), detraining (DeTr), and detraining with BCAA supplementation (DeTr + BCAA). Mice in Tr, DeTr, and DeTr + BCAA performed treadmill running exercises [20-30 m/min, 60 min, 5 times/week, 4 weeks]. Then, mice in DeTr and DeTr + BCAA were administered with water or BCAA [0.6 mg/g of body weight, twice daily] for 2 weeks of detraining. In whole skeletal muscle, mitochondrial enzyme activities and protein content were decreased after 2 weeks of detraining, but the reduction was suppressed by BCAA supplementation. Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) protein content, a master regulator of mitochondrial biogenesis, was decreased by detraining irrespective of BCAA ingestion. Regarding mitochondrial degradation, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), a mitophagy-related protein, was significantly higher in the Tr group than in the DeTr + BCAA group, but not different from in the DeTr group. With respect to mitochondrial quality, BCAA ingestion did not affect oxygen consumption rate (OCR) and reactive oxygen species (ROS) production in isolated mitochondria. Our findings suggest that BCAA ingestion suppresses the detraining-induced reduction of mitochondrial content partly through inhibiting mitophagy.
Scientific journal, English - Effect of endurance training and PGC-1α overexpression on calculated lactate production volume during exercise based on blood lactate concentration.
Reo Takeda; Yudai Nonaka; Katsuyuki Kakinoki; Shinji Miura; Yutaka Kano; Daisuke Hoshino
Scientific reports, 12, 1, 1635-1635, 31 Jan. 2022, Peer-reviwed, True, Lactate production is an important clue for understanding metabolic and signal responses to exercise but its measurement is difficult. Therefore, this study aimed (1) to develop a method of calculating lactate production volume during exercise based on blood lactate concentration and compare the effects between endurance exercise training (EX) and PGC-1α overexpression (OE), (2) to elucidate which proteins and enzymes contribute to changes in lactate production due to EX and muscle PGC-1α OE, and (3) to elucidate the relationship between lactate production volume and signaling phosphorylations involved in mitochondrial biogenesis. EX and PGC-1α OE decreased muscle lactate production volume at the absolute same-intensity exercise, but only PGC-1α OE increased lactate production volume at the relative same-intensity exercise. Multiple linear regression revealed that phosphofructokinase, monocarboxylate transporter (MCT)1, MCT4, and citrate synthase equally contribute to the lactate production volume at high-intensity exercise within physiological adaptations, such as EX, not PGC-1α OE. We found that an exercise intensity-dependent increase in the lactate production volume was associated with a decrease in glycogen concentration and an increase in P-AMPK/T-AMPK. This suggested that the calculated lactate production volume was appropriate and reflected metabolic and signal responses but further modifications are needed for the translation to humans.
Scientific journal, English - Short-Term Calorie Restriction Maintains Plasma Insulin Concentrations along with a Reduction in Hepatic Insulin-Degrading Enzyme Levels in db/db Mice.
Yudai Nonaka; Reo Takeda; Yutaka Kano; Daisuke Hoshino
Nutrients, 13, 4, 03 Apr. 2021, Peer-reviwed, True, Maintaining blood insulin levels is important for patients with diabetes because insulin secretion capacity declines with the development of the disease. Calorie restriction (CR) is effective for the improvement of glucose tolerance, but it is not clear whether CR can maintain insulin levels in the late stage of diabetes. We examined the effect of CR on whole-body glucose tolerance and fasting blood insulin concentrations in the late stage of diabetes. Male db/db mice were subjected to either a standard laboratory diet ad libitum for 3 weeks (dbdb group) or 40% CR (dbdb+CR group). CR significantly decreased body mass and epididymal fat weight. Glucose tolerance and fasting glucose levels were significantly improved with 3-week CR. Fasting insulin concentrations were decreased in the dbdb group but were maintained in the dbdb+CR group. CR significantly reduced insulin-degrading enzyme (IDE) levels in the liver, and hepatic IDE levels were significantly positively and negatively correlated with plasma glucose concentrations (area under the curve) after glucose administration and after fasting insulin concentrations, respectively. Therefore, 3-week CR maintained blood insulin levels and improved glucose tolerance with decreased hepatic IDE levels in an animal model of late-stage diabetes.
Scientific journal, English - In vivo Ca2+ dynamics during cooling after eccentric contractions in rat skeletal muscle.
Ryo Takagi; Ayaka Tabuchi; Tomoyo Asamura; Seiya Hirayama; Ryo Ikegami; Yoshinori Tanaka; Daisuke Hoshino; David C Poole; Yutaka Kano
American journal of physiology. Regulatory, integrative and comparative physiology, 320, 2, R129-R137, 01 Feb. 2021, Peer-reviwed, True, The effect of cooling on in vivo intracellular calcium ion concentration [Ca2+]i after eccentric contractions (ECs) remains to be determined. We tested the hypothesis that cryotherapy following ECs promotes an increased [Ca2+]i and induces greater muscle damage in two muscles with substantial IIb and IIx fiber populations. The thin spinotrapezius (SPINO) muscles of Wistar rats were used for in vivo [Ca2+]i imaging, and tibialis anterior (TA) muscles provided greater fidelity and repeatability of contractile function measurements. SPINO [Ca2+]i was estimated using fura 2-AM and the magnitude, location, and temporal profile of [Ca2+]i determined as the temperature near the muscle surface post-ECs was decreased from 30°C (control) to 20°C or 10°C. Subsequently, in the TA, the effect of post-ECs cooling to 10°C on muscle contractile performance was determined at 1 and 2 days after ECs. TA muscle samples were examined by hematoxylin and eosin staining to assess damage. In SPINO, reducing the muscle temperature from 30°C to 10°C post-ECs resulted in a 3.7-fold increase in the spread of high [Ca2+]i sites generated by ECs (P < 0.05). These high [Ca2+]i sites demonstrated partial reversibility when rewarmed to 30°C. Dantrolene, a ryanodine receptor Ca2+ release inhibitor, reduced the presence of high [Ca2+] sites at 10°C. In the TA, cooling exacerbated ECs-induced muscle strength deficits via enhanced muscle fiber damage (P < 0.05). By demonstrating that cooling post-ECs potentiates [Ca2+]i derangements, this in vivo approach supports a putative mechanistic basis for how postexercise cryotherapy might augment muscle fiber damage and decrease subsequent exercise performance.
Scientific journal, English - Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes.
Daisuke Hoshino; Kentaro Kawata; Katsuyuki Kunida; Atsushi Hatano; Katsuyuki Yugi; Takumi Wada; Masashi Fujii; Takanori Sano; Yuki Ito; Yasuro Furuichi; Yasuko Manabe; Yutaka Suzuki; Nobuharu L Fujii; Tomoyoshi Soga; Shinya Kuroda
iScience, 23, 10, 101558-101558, 23 Oct. 2020, Peer-reviwed, True, Skeletal muscle adaptation is mediated by cooperative regulation of metabolism, signal transduction, and gene expression. However, the global regulatory mechanism remains unclear. To address this issue, we performed electrical pulse stimulation (EPS) in differentiated C2C12 myotubes at low and high frequency, carried out metabolome and transcriptome analyses, and investigated phosphorylation status of signaling molecules. EPS triggered extensive and specific changes in metabolites, signaling phosphorylation, and gene expression during and after EPS in a frequency-dependent manner. We constructed trans-omic network by integrating these data and found selective activation of the pentose phosphate pathway including metabolites, upstream signaling molecules, and gene expression of metabolic enzymes after high-frequency EPS. We experimentally validated that activation of these molecules after high-frequency EPS was dependent on reactive oxygen species (ROS). Thus, the trans-omic analysis revealed ROS-dependent activation in signal transduction, metabolome, and transcriptome after high-frequency EPS in C2C12 myotubes, shedding light on possible mechanisms of muscle adaptation.
Scientific journal, English - Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise.
Takumi Wada; Ken-Ichi Hironaka; Mitsutaka Wataya; Masashi Fujii; Miki Eto; Shinsuke Uda; Daisuke Hoshino; Katsuyuki Kunida; Haruki Inoue; Hiroyuki Kubota; Tsuguto Takizawa; Yasuaki Karasawa; Hirofumi Nakatomi; Nobuhito Saito; Hiroki Hamaguchi; Yasuro Furuichi; Yasuko Manabe; Nobuharu L Fujii; Shinya Kuroda
Cell reports, 32, 9, 108051-108051, 01 Sep. 2020, Peer-reviwed, True, Cell-to-cell variability in signal transduction in biological systems is often considered noise. However, intercellular variation (i.e., cell-to-cell variability) has the potential to enable individual cells to encode different information. Here, we show that intercellular variation increases information transmission of skeletal muscle. We analyze the responses of multiple cultured myotubes or isolated skeletal muscle fibers as a multiple-cell channel composed of single-cell channels. We find that the multiple-cell channel, which incorporates intercellular variation as information, not noise, transmitted more information in the presence of intercellular variation than in the absence according to the "response diversity effect," increasing in the gradualness of dose response by summing the cell-to-cell variable dose responses. We quantify the information transmission of human facial muscle contraction during intraoperative neurophysiological monitoring and find that information transmission of muscle contraction is comparable to that of a multiple-cell channel. Thus, our data indicate that intercellular variation can increase the information capacity of tissues.
Scientific journal, English - Effects of electrical stimulation-induced resistance exercise training on white and brown adipose tissues and plasma meteorin-like concentration in rats.
Yuhei Amano; Yudai Nonaka; Reo Takeda; Yutaka Kano; Daisuke Hoshino
Physiological reports, 8, 16, e14540, Aug. 2020, Peer-reviwed, True, Chronic endurance exercise training induces morphological and metabolic alterations including mitochondrial biogenesis in white adipose tissue (WAT) and brown adipose tissue (BAT) in rodents. A myokine called meteorin-like (Metrnl) is associated with morphological and metabolic adaptation and increased in blood after acute resistance exercise. However, the effects of chronic resistance exercise training (RT), which aims to increase muscle mass and strength, on WAT and BAT are unclear. Therefore, we aimed to clarify the effects of RT on morphological and metabolic parameters in WAT and BAT and on plasma Metrnl concentrations. We applied electrical stimulation to both legs of rats as RT three times a week for 4 weeks. RT reduced adipocyte size in subcutaneous WAT but induced no changes in mitochondrial and thermogenesis proteins. In BAT, peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) protein levels and mitochondrial content markers were significantly higher in the RT group compared with the control group. A significant positive correlation was found between the expression of PGC-1α in BAT and plasma Metrnl concentrations. These results suggest that plasma Metrnl is associated with PGC-1α and mitochondrial biogenesis in BAT. This study describes a potential role of RT in preventing metabolic diseases via altering WAT and BAT and increasing plasma Mertnl concentrations.
Scientific journal, English - Effects of acute 3-h swimming exercise on insulin secretion capacity of pancreatic islets
Yudai Nonaka; Reo Takeda; Yutaka Kano; Daisuke Hoshino
The Journal of Physical Fitness and Sports Medicine, The Japanese Society of Physical Fitness and Sports Medicine, 9, 4, 173-179, 25 Jul. 2020, Peer-reviwed
Scientific journal - Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics.
Naoki Matsuda; Ken-ichi Hironaka; Masashi Fujii; Takumi Wada; Katsuyuki Kunida; Haruki Inoue; Miki Eto; Daisuke Hoshino; Yasuro Furuichi; Yasuko Manabe; Nobuharu L. Fujii; Hiroyuki Noji; Hiromi Imamura; Shinya Kuroda
Quant. Biol., 8, 3, 228-237, 2020, Peer-reviwed
Scientific journal - Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle.
Yu Kitaoka; Daiki Watanabe; Yudai Nonaka; Kazuyoshi Yagishita; Yutaka Kano; Daisuke Hoshino
Physiol Rep, Wiley, 7, 19, e14266-e14266, Oct. 2019, Peer-reviwed, Clenbuterol induces a slow-to-fast fiber type transition in skeletal muscle. This muscle fiber transition decreased mitochondrial oxidative capacity and respiratory function. We hypothesized that the clenbuterol-mediated reduction in oxidative capacity is associated with the alteration in mitochondrial morphology. To verify this hypothesis, we examined whether clenbuterol alters mitochondrial morphology and mitochondrial regulatory proteins in rat skeletal muscle. Clenbuterol was administered to rats via drinking water (30 mg/L) for 3 weeks. Myosin heavy chain (MHC) isoform composition, mitochondrial morphology, and fusion and fission regulatory protein levels in deep region and superficial region in tibialis anterior (TA) muscles were assessed. Clenbuterol induced the fiber type transition from slow to fast in both the regions of TA. The levels of optic atrophy protein 1, mitofusin 2, and mitochondrial fission 1, but not of dynamin-related protein 1, significantly decreased in deep and superficial muscles after clenbuterol administration (P < 0.01). Also, observation using the transmission electron microscopy showed a decrease in mitochondrial volume (P < 0.05) and an increa
Scientific journal, English - Effects of low-carbohydrate diet and resistance exercise training on physical characteristics and concentrations of plasma metabolites and hormones
Sho Ito; Yasuaki Karasawa; Daisuke Hoshino; Masashi Fujii; Miki Eto; Junya Tsuru; Chieko Kashiwado; Shinya Kuroda
Japanese Journal of Physical Fitness and Sports Medicine, The Japanese Society of Physical Fitness and Sports Medicine, 68, 3, 223-227, 01 Jun. 2019, Peer-reviwed
Scientific journal - Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions.
Ryo Ikegami; Hiroaki Eshima; Takuro Mashio; Tomosada Ishiguro; Daisuke Hoshino; David C Poole; Yutaka Kano
Journal of applied physiology (Bethesda, Md. : 1985), 126, 3, 691-698, 01 Mar. 2019, Peer-reviwed, True, Heat stress promotes intramyocyte calcium concentration ([Ca2+]i) accumulation via transient receptor potential vanilloid 1 (TRPV1) channels. We tested the hypothesis that muscle contractile activity concomitant with heat stress would accelerate the increase in [Ca2+]i via TRPV1, further impairing [Ca2+]i homeostasis. Spinotrapezius muscles of adult Wistar rats were exteriorized in vivo and loaded with the fluorescent Ca2+ probe fura 2-AM. Heat stress (muscle surface temperature 40°C) was used as TRPV1 activator. An isometric contraction (100 Hz, 5-10 V, 30 s) was induced electrically concomitant with heat stress. [Ca2+]i was determined for 20 min using in vivo fluorescence microscopy, and the phosphorylation response of TRPV1 was determined by Western blotting. Heat stress induced a significant [Ca2+]i increase of 18.5 ± 8.1% at 20 min and TRPV1 phosphorylation (+231%), which was inhibited by addition of the TRPV1 inhibitor (capsazepine). However, contrary to expectations, the heat stress and isometric contraction condition almost completely inhibited TRPV1 phosphorylation and the consequent [Ca2+]i elevation (<2.8% accumulation during heat stress, P > 0.05). In conclusion, this in vivo physiological model demonstrated that isometric muscle contraction(s) can suppress the phosphorylation response of TRPV1 and maintain [Ca2+]i homeostasis during heat stress. NEW & NOTEWORTHY This investigation is the first document the dynamics of intramyocyte calcium concentration ([Ca2+]i) increase in the myoplasm of skeletal muscle fibers in response to heat stress where the muscle blood flow is preserved. Heat stress at 40°C drives a myoplasmic [Ca2+]i accumulation in concert with transient receptor potential vanilloid 1 (TRPV1) phosphorylation. However, muscle contraction caused TRPV1 channel deactivation by dephosphorylation of TRPV1. TRPV1 inactivation via isometric contraction(s) permits maintenance of [Ca2+]i homeostasis even under high imposed muscle temperature.
Scientific journal, English - Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation.
Inoue H; Kunida K; Matsuda N; Hoshino D; Wada T; Imamura H; Noji H; Kuroda S
Cell structure and function, 43, 2, 153-169, Aug. 2018, Peer-reviwed, False, Automatic cell segmentation is a powerful method for quantifying signaling dynamics at single-cell resolution in live cell fluorescence imaging. Segmentation methods for mononuclear and round shape cells have been developed extensively. However, a segmentation method for elongated polynuclear cells, such as differentiated C2C12 myotubes, has yet to be developed. In addition, myotubes are surrounded by undifferentiated reserve cells, making it difficult to identify background regions and subsequent quantification. Here we developed an automatic quantitative segmentation method for myotubes using watershed segmentation of summed binary images and a two-component Gaussian mixture model. We used time-lapse fluorescence images of differentiated C2C12 cells stably expressing Eevee-S6K, a fluorescence resonance energy transfer (FRET) biosensor of S6 kinase (S6K). Summation of binary images enhanced the contrast between myotubes and reserve cells, permitting detection of a myotube and a myotube center. Using a myotube center instead of a nucleus, individual myotubes could be detected automatically by watershed segmentation. In addition, a background correction using the two-component Gaussian mixture model permitted automatic signal intensity quantification in individual myotubes. Thus, we provide an automatic quantitative segmentation method by combining automatic myotube detection and background correction. Furthermore, this method allowed us to quantify S6K activity in individual myotubes, demonstrating that some of the temporal properties of S6K activity such as peak time and half-life of adaptation show different dose-dependent changes of insulin between cell population and individuals.Key words: time lapse images, cell segmentation, fluorescence resonance energy transfer, C2C12, myotube.
Scientific journal, English - Selective control of up-regulated and down-regulated genes by temporal patterns and doses of insulin
Takanori Sano; Kentaro Kawata; Satoshi Ohno; Katsuyuki Yugi; Hiroaki Kakuda; Hiroyuki Kubota; Shinsuke Uda; Masashi Fujii; Katsuyuki Kunida; Daisuke Hoshino; Atsushi Hatano; Yuki Ito; Miharu Sato; Yutaka Suzuki; Shinya Kuroda
Science Signaling, 9, 455, ra112, Nov. 2016, Peer-reviwed
Scientific journal, English - Exercise-induced expression of monocarboxylate transporter 2 in the cerebellum and its contribution to motor performance
Daisuke Hoshino; Susumu Setogawa; Yu Kitaoka; Hiroyuki Masuda; Yuki Tamura; Hideo Hatta; Dai Yanagihara
NEUROSCIENCE LETTERS, 633, 1-6, Oct. 2016, Peer-reviwed
Scientific journal, English - High-intensity interval training enhances oxidative capacity and substrate availability in skeletal muscle
Hoshino Daisuke; Kitaoka Yu; Hatta Hideo
The Journal of Physical Fitness and Sports Medicine, 一般社団法人日本体力医学会, 5, 1, 13-23, 2016, Peer-reviwed, High-intensity interval training (HIIT) consists of repeated short bursts of high-intensity exercise and rest. Here we review recent work focusing on the metabolic adaptations to HIIT, especially in oxidative capacity and substrate availability in skeletal muscle. In this review, HIIT is defined as chronic training, for at least 2 weeks, involving repeated short-duration high-intensity exercise at >85% VO2 max, followed by complete rest or active rest, for any given duration of exercise and rest. First, we describe the effects of HIIT on muscle substrate oxidative metabolism, specifically in terms of mitochondria and substrate transporters. HIIT changes muscle mitochondrial content, function and dynamics. HIIT increases the protein content of transporters of glucose, lactate and fatty acids in skeletal muscle. These adaptations of mitochondria and transporter proteins improve oxidative capacity and substrate availability in skeletal muscle. Second, we introduce a potential mechanism of HIIT-induced adaptations in skeletal muscle, focusing on mitochondrial biogenesis. It is well known that a mechanism of mitochondrial biogenesis involves PGC-1alpha protein and its upstream signaling pathways including Ca2+/calmodulin-dependent protein kinase, AMP-activated protein kinase and mitogen-activated protein kinase p38. Given that mitochondrial biogenesis occurs in an exercise-intensity-dependent manner, mobilization of fast-twitch fibers and lactate accumulation are important. Finally, we discuss the future direction of HIIT research, involving systems biology approaches such as omics technologies and mathematical modeling, which may overcome current limitations and accelerate our understanding of mechanisms of HIIT-induced adaptations.
English - Effects of decreased lactate accumulation after dichloroacetate administration on exercise training-induced mitochondrial adaptations in mouse skeletal muscle.
Daisuke Hoshino; Yuki Tamura; Hiroyuki Masuda; Yutaka Matsunaga; Hideo Hatta
Physiological reports, 3, 9, Sep. 2015, Peer-reviwed, True, Recent studies suggested that lactate accumulation can be a signal for mitochondrial biogenesis in skeletal muscle. We investigated whether reductions in lactate concentrations in response to dichloroacetate (DCA), an activator of pyruvate dehydrogenase, attenuate mitochondrial adaptations after exercise training in mice. We first confirmed that DCA administration (200 mg/kg BW by i.p. injection) 10 min before exercise decreased muscle and blood lactate concentrations after high-intensity interval exercise (10 bouts of 1 min treadmill running at 40 m/min with a 1 min rest). At the same time, exercise-induced signal cascades did not change by pre-exercise DCA administration. These results suggested that DCA administration affected only lactate concentrations after exercise. We next examined the effects of acute DCA administration on mRNA expressions involved with mitochondrial biogenesis after same high-intensity interval exercise and the effects of chronic DCA administration on mitochondrial adaptations after high-intensity interval training (increasing intensity from 38 to 43 m/min by the end of training period). Acute DCA administration did not change most of the exercise-induced mRNA upregulation. These data suggest that lactate reductions by DCA administration did not affect transcriptional activation after high-intensity interval exercise. However, chronic DCA administration attenuated, in part, mitochondrial adaptations such as training-induced increasing rates of citrate synthase (P = 0.06), β-hydroxyacyl CoA dehydrogenase activity (P < 0.05), cytochrome c oxidase IV (P < 0.05) and a fatty acid transporter, fatty acid translocase/CD36 (P < 0.05), proteins after exercise training. These results suggest that lactate accumulation during high-intensity interval exercise may be associated with mitochondrial adaptations after chronic exercise training.
Scientific journal, English - Daily heat stress treatment rescues denervation-activated mitochondrial clearance and atrophy in skeletal muscle
Yuki Tamura; Yu Kitaoka; Yutaka Matsunaga; Daisuke Hoshino; Hideo Hatta
JOURNAL OF PHYSIOLOGY-LONDON, 593, 12, 2707-2720, Jun. 2015, Peer-reviwed
Scientific journal, English - Pre-exercise casein peptide supplementation enhances endurance training-induced mitochondrial enzyme activity in slow twitch muscle, but not fast twitch muscle of high fat diet-fed mice
Matsunaga Yutaka; Tamura Yuki; Takahashi Yumiko; Masuda Hiroyuki; Hoshino Daisuke; Kitaoka Yu; Saito Noriko; Nakamura Hirohiko; Takeda Yasuhiro; Hatta Hideo
JPFSM, The Japanese Society of Physical Fitness and Sports Medicine, 4, 5, 377-384, 2015, Peer-reviwed - Chronic Post-Exercise Lactate Administration with Endurance Training Increases Glycogen Concentration and Monocarboxylate Transporter 1 Protein in Mouse White Muscle
Daisuke Hoshino; Tatsuya Hanawa; Yumiko Takahashi; Hiroyuki Masuda; Mai Kato; Hideo Hatta
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 60, 6, 413-419, Dec. 2014, Peer-reviwed
Scientific journal, English - Postexercise whole body heat stress additively enhances endurance training-induced mitochondrial adaptations in mouse skeletal muscle
Yuki Tamura; Yutaka Matsunaga; Hiroyuki Masuda; Yumiko Takahashi; Yuki Takahashi; Shin Terada; Daisuke Hoshino; Hideo Hatta
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 307, 7, R931-R943, Oct. 2014, Peer-reviwed
Scientific journal, English - High-intensity interval training increases intrinsic rates of mitochondrial fatty acid oxidation in rat red and white skeletal muscle
Daisuke Hoshino; Yuko Yoshida; Yu Kitaoka; Hideo Hatta; Arend Bonen
Applied Physiology, Nutrition and Metabolism, 3, 38, 3, 326-333, 2013, Peer-reviwed
Scientific journal, English - Monocarboxylate transporter and lactate metabolism
KITAOKA Yu; HOSHINO Daisuke; HATTA Hideo
JPFSM, The Japanese Society of Physical Fitness and Sports Medicine, 1, 2, 247-252, Jul. 2012, Peer-reviwed, For many years, lactate was considered as a metabolic waste product, and a cause of fatigue during exercise. However, lactate is now known as a carbohydrate fuel source, shuttled between cells and tissues. The movement of lactate across the plasma membrane is facilitated by a family of monocarboxylate transporters (MCTs). Among 14 identified MCT isoforms, MCT1 and MCT4 are present in the plasma membranes of skeletal muscle and are suggested to be related to exercise performance. Reports have shown that exercise training increases both MCT1 and MCT4 in skeletal muscle. This review will discuss the role of lactate as a fuel and exercise training-induced changes in lactate metabolism. In addition, studies of Thoroughbred horses, as models of elite athletes, are introduced.
Scientific journal, English - Clenbuterol, a beta 2-adrenergic agonist, reciprocally alters PGC-1 alpha and RIP140 and reduces fatty acid and pyruvate oxidation in rat skeletal muscle
Daisuke Hoshino; Yuko Yoshida; Graham P. Holloway; James Lally; Hideo Hatta; Arend Bonen
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 302, 3, R373-R384, Feb. 2012, Peer-reviwed
Scientific journal, English - Effect of growth on monocarboxylate transporters and indicators of energy metabolism in the gluteus medius muscle of Thoroughbreds
Yu Kitaoka; Daisuke Hoshino; Kazutaka Mukai; Atsushi Hiraga; Tohru Takemasa; Hideo Hatta
AMERICAN JOURNAL OF VETERINARY RESEARCH, 72, 8, 1107-1111, Aug. 2011, Peer-reviwed
Scientific journal, English - Higher Lactate Transporter Protein and Citrate Synthase Activity Following Short-term High-intensity Repetition Training in Mice
Hoshino Daisuke; Matsumae Haruka; Kato Mai; Hatta Hideo
Int. J. Sport Health Sci., Japan Society of Physical Education, Health and Sport Sciences, 8, 43-49, 2010, Peer-reviwed, The purpose of this study was to examine the effects of high-intensity repetition training, consisting of 4 bouts of high-intensity exercise with long rest periods, on lactate metabolism. To achieve this goal, we compared effects of 3 weeks of two different exercise regimes: high-intensity repetition training (4×1 min run at 45-53 m/min with 19 min of recovery=total 36 running bouts of 1 min), and voluntary wheel running activity, which mimics endurance training. To evaluate the effects on lactate metabolism, we measured protein levels of the monocarboxylate transporters (MCT). We also measured citrate synthase activity as a marker of oxidative capacity in working muscle. After 3 weeks of training, repetition training, but not wheel running training, increased the levels of MCT1 protein, which contributes to lactate uptake into skeletal muscle, in soleus (p<0.05) and also muscular citrate synthase activity in tibialis anterior (p<0.05). MCT4 protein, which plays the role in the removal of lactate from muscle, did not change in plantaris or tibialis anterior following either training regime. These data suggest that short-term high-intensity repetition training is effective at increasing MCT1 and mitochondrial oxidative capacity in skeletal muscle compared to running wheel activity.
English - Effects of high-intensity training on monocarboxylate transporters in Thoroughbred horses
Yu Kitaoka; Yuki Wakasugi; Daisuke Hoshino; Kazutaka Mukai; Atsushi Hiraga; Hideo Hatta
Comparative Exercise Physiology, 6, 4, 171-175, 2009, Peer-reviwed
Scientific journal, English
MISC
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草野達哉; 曽谷祐太; 竹田怜央; 幡野敦; 川田健太郎; 狩野豊; 星野太佑
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402210222809558 - 老齢マウス骨格筋におけるヒト特異的CMAH遺伝子欠損の影響
田渕絢香; 竹田怜央; 星野太佑; 星野太佑; 狩野豊; 狩野豊
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402218700915548 - 4週間の高脂肪食摂取は筋収縮による遺伝子発現に影響を与える
根橋諒; 川田健太郎; 竹田怜央; 曽谷祐太; 草野達哉; 狩野豊; 星野太佑
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402253239165444 - ピルビン酸投与は高脂肪食摂取による糖代謝異常を改善させる
安藤泰地; 竹田怜央; 狩野遼太郎; 狩野遼太郎; 草野達哉; 野中雄大; 狩野豊; 星野太佑
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402260308093561 - 豊かな環境における自発性身体活動量と海馬における発現変動遺伝子の関係
須藤みず紀; 竹田怜央; 草野達哉; 星野太佑; 安藤創一; 狩野豊
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402266863366961 - 筋芽細胞の分化過程での冷却感受性の変化
薮田洋也; 長名シオン; 白川英樹; 星野太佑; 狩野豊
2024, 日本運動生理学会大会プログラム・抄録集, 32nd, 202402271583192431 - 高圧高酸素処置が運動後の筋グリコーゲン回復に及ぼす影響
野中 雄大; 竹田 怜央; 小間 陸嗣; 狩野 遼太郎; 柳下 和慶; 芝口 翼; 増田 和実; 狩野 豊; 星野 太佑
(一社)日本体力医学会, Aug. 2023, 体力科学, 72, 4, 331-331, Japanese, 0039-906X, 1881-4751, 2024025669 - トレーニング休止期間中のBCAA摂取が骨格筋ミトコンドリアおよびエネルギー基質利用に与える影響
松永 裕; 田村 優樹; 高橋 謙也; 北岡 祐; 高橋 祐美子; 星野 太佑; 八田 秀雄
(NPO)日本スポーツ栄養学会, Jan. 2023, 日本スポーツ栄養研究誌, 16, 90-90, Japanese, 2188-8922, 2023149612 - 【健康寿命の鍵を握る骨格筋 代謝・内分泌を介した全身性制御の分子基盤から運動による抗老化まで】(第5章)骨格筋の秘めた特徴を捉える新技術 筋収縮後の代謝トランスオミクスネットワークの構築 ROS依存的なペントースリン酸経路の活性化
星野 太佑; 川田 健太郎; 黒田 真也
(株)羊土社, Feb. 2022, 実験医学, 40, 2, 288-294, Japanese, 0288-5514, 2022097998 - Cmahの欠損は老齢マウスにおける脂肪組織の細胞老化およびインスリン感受性を改善させる
竹田怜央; 田渕絢香; 野中雄大; 狩野遼太郎; 狩野豊; 星野太佑
2022, 体力科学, 71, 4, 0039-906X, 202202287482851764 - ラットへの電気刺激によるレジスタンストレーニングが脂肪細胞へ与える影響
天野 悠平; 星野 太佑
(一社)日本肥満学会, Oct. 2019, 肥満研究, 25, Suppl., 311-311, Japanese, 1343-229X, 2020234624 - Single-cell information analysis reveals small intra- and large intercellular variations increase cellular information capacity
Wada T; Wataya M; Fujii M; Hironaka K; Eto M; Uda S; Hoshino D; Kunida K; Inoue H; Kubota H; Hamaguchi H; Furuichi Y; Manabe Y; Fujii N; Kuroda S
May 2019, bioRxiv - 電気刺激時C2C12筋管細胞におけるミトコンドリアATPのダイナミクスとその応答特性
松田 直樹; 国田 勝行; 和田 卓巳; 井上 晴幾; 星野 太佑; 黒田 真也
(一社)日本細胞生物学会, May 2017, 日本細胞生物学会大会講演要旨集, 69回, 117-117, Japanese, 2018261293 - 高強度インターバルトレーニングによる骨格筋ミトコンドリアの適応と乳酸
星野 太佑; 八田 秀雄
(一社)日本体力医学会, Feb. 2017, 体力科学, 66, 1, 121-121, Japanese, 0039-906X, 1881-4751, 2017230344 - インスリン時間パターン依存的な遺伝子発現の選択的応答
佐野 貴規; 川田 健太郎; 角田 裕晶; 久保田 浩行; 宇田 新介; 柚木 克之; 藤井 雅史; 国田 勝行; 星野 太佑; 鈴木 穣; 黒田 真也
(公社)日本生化学会, Dec. 2015, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [1P0791]-[1P0791], Japanese, 2017067935 - B-1-3 Heat stress treatment rescues denervation-induced mitochondrial loss in skeletal muscle(The Proceedings of the 23rd Annual Meetings of Japan Society of Exercise and Sports Physiology July 25・26, (Tokyo)) :
Tamura Yuki; Kitaoka Yu; Matsunaga Yutaka; Hoshino Daisuke; Hatta Hideo
Japan Society of Exercise and Sports Physiology, 2015, Advances in exercise and sports physiology, 21, 4, 77-77, English, 1340-3141, 110010015279, AA11107566 - カゼインペプチドの摂取は持久的トレーニングの効果を高める
松永 裕; 田村 優樹; 高橋 祐美子; 増田 紘之; 星野 太佑; 八田 秀雄
(一社)日本体力医学会, Dec. 2013, 体力科学, 62, 6, 537-537, Japanese, 0039-906X, 1881-4751, 2014155449 - 抗酸化物質豊富な食物の摂取が持久的トレーニング効果に与える影響
皆川 秀洋; 星野 太佑; 増田 紘之; 高橋 祐美子; 田村 優樹; 松永 裕; 八田 秀雄
(一社)日本体力医学会, Dec. 2013, 体力科学, 62, 6, 539-539, Japanese, 0039-906X, 1881-4751, 2014155460 - 運動後の温熱刺激は、持久的トレーニングによる骨格筋ミトコンドリアの適応を増強する
田村 優樹; 松永 裕; 高橋 祐美子; 増田 紘之; 星野 太佑; 八田 秀雄
(一社)日本体力医学会, Dec. 2013, 体力科学, 62, 6, 571-571, Japanese, 0039-906X, 1881-4751, 2014155588 - ジクロロ酢酸摂取による運動中の乳酸濃度の低下が骨格筋のエネルギー代謝の適応に与える影響
星野 太佑; 田村 優樹; 増田 紘之; 八田 秀雄
(一社)日本体力医学会, Dec. 2012, 体力科学, 61, 6, 626-626, Japanese, 0039-906X, 1881-4751, 2013162735 - High-intensity interval training increases the rate of mitochondrial fatty acid oxidation in rat skeletal muscle
HOSHINO Daisuke; YOSHIDA Yuko; HATTA Hideo
01 Feb. 2012, 体力科學, 61, 1, 55-55, English, 0039-906X, 10030139788, AN00137986 - カゼインペプチドの長期摂取がエネルギー代謝に及ぼす影響
松永裕; 田村優樹; 増田紘之; 高橋祐美子; 星野太佑; 八田秀雄
2012, 体力科学, 61, 6, 0039-906X, 201302250175802178 - 2-A-09 Single oral taurine supplementation activates muscleglycogen resynthesis during period of recovery from exhaustive exercise(The Proceedings of the 19th Annual Meetings of Japan Society of Exercise and Sports Physiology August 25・26, (Tokushima)) :
Takahashi Yumiko; Hoshino Daisuke; Hatta Hideo
Japan Society of Exercise and Sports Physiology, 2011, Advances in exercise and sports physiology, 17, 2, 54-54, English, 1340-3141, 110009437974, AA11107566 - 2-A-08 Changes in protein contents of PGC-1α and RIP140 and metabolic adaptation after three weeks of clenbuterol administration(The Proceedings of the 19th Annual Meetings of Japan Society of Exercise and Sports Physiology August 25・26, (Tokushima)) :
HOSHINO Daisuke; HATTA Hideo
Japan Society of Exercise and Sports Physiology, 2011, Advances in exercise and sports physiology, 17, 2, 54-54, English, 1340-3141, 110009437973, AA11107566 - 持久的トレーニングがモノカルボン酸トランスポーター(MCT)を中心とする乳酸代謝に与える影響
増田紘之; 増田毅; 星野太佑; 北岡祐; 高橋祐美子; 八田秀雄
2010, 体力科学, 59, 6, 0039-906X, 201102223241694878 - 265. サラブレッドにおける発育発達によるMCTタンパク質量の変化(代謝,一般口演,第63回日本体力医学会大会)
北岡 祐; 星野 太佑; 向井 和隆; 平賀 敦; 八田 秀雄
日本体力医学会, 01 Dec. 2008, 体力科學, 57, 6, 765-765, Japanese, 0039-906X, 110007164556, AN00137986 - 273. ビタミンB1誘導体の投与が、安静時及び持久的運動時における糖代謝に与える影響(代謝,一般口演,第63回日本体力医学会大会)
増田 紘之; 増田 毅; 星野 太佑; 北岡 祐; 加藤 麻衣; 八田 秀雄
日本体力医学会, 01 Dec. 2008, 体力科學, 57, 6, 769-769, Japanese, 0039-906X, 110007164789, AN00137986 - 244. 高強度のレペティショントレーニングは糖の利用を増加させる(代謝,一般口演,第63回日本体力医学会大会)
星野 太佑; 加藤 麻衣; 増田 紘之; 北岡 祐; 八田 秀雄
日本体力医学会, 01 Dec. 2008, 体力科學, 57, 6, 0039-906X, 110007165072 - 194. 3週間の高強度トレーニングがマウスの乳酸代謝に与える影響(代謝,第62回日本体力医学会大会)
星野 太佑; 松前 遥; 加藤 麻衣; 増田 紘之; 八田 秀雄
日本体力医学会, 01 Dec. 2007, 体力科學, 56, 6, 675-675, Japanese, 0039-906X, 110006652488, AN00137986 - 206. 乳酸の長期投与が運動中の乳酸を中心とする糖代謝に及ぼす影響(代謝,第62回日本体力医学会大会)
加藤 麻衣; 星野 太佑; 松前 遥; 増田 紘之; 八田 秀雄
日本体力医学会, 01 Dec. 2007, 体力科學, 56, 6, 0039-906X, 110006652500 - 207. フルスルチアミン投与が持久的運動における乳酸を中心とする糖代謝に与える影響(代謝,第62回日本体力医学会大会)
増田 紘之; 松前 遥; 星野 太佑; 加藤 麻衣; 八田 秀雄
日本体力医学会, 01 Dec. 2007, 体力科學, 56, 6, 682-682, Japanese, 0039-906X, 110006652501, AN00137986 - 208. サラブレッドの各組織におけるMCTタンパク質の分布(代謝,第62回日本体力医学会大会)
北岡 祐; 星野 太佑; 向井 和隆; 平賀 敦; 八田 秀雄
日本体力医学会, 01 Dec. 2007, 体力科學, 56, 6, 682-682, Japanese, 0039-906X, 110006652502, AN00137986 - 3. Effect of one week of high intensity training on lactate metabolism in mice(The Proceedings of the 15th Annual Meetings of Japan Society of Exercise and Sports Physiology July 26-27, (Hirosaki)) :
HOSHINO Daisuke; MATSUMAE Haruka; HATTA Hideo
Japan Society of Exercise and Sports Physiology, 2007, Advances in exercise and sports physiology, 13, 3, 56-56, English, 1340-3141, 110006570705, AA11107566 - 6. Effect of TTFD administration on glucose and lactate metabolism in mice during high intensity exercise(The Proceedings of the 15th Annual Meetings of Japan Society of Exercise and Sports Physiology July 26-27, (Hirosaki)) :
MATSUMAE Haruka; HOSHINO Daisuke; HATTA Hideo
Japan Society of Exercise and Sports Physiology, 2007, Advances in exercise and sports physiology, 13, 3, 57-57, English, 1340-3141, 110006570708, AA11107566
Research Themes
- 乳酸が骨格筋の適応を引き起こす新たな基盤創出とその応用
八田 秀雄; 北岡 祐; 高橋 謙也; 星野 太佑
日本学術振興会, 科学研究費助成事業, 東京大学, 基盤研究(B), 24K02812
01 Apr. 2024 - 31 Mar. 2028 - 乳酸による適応のメカニズム解明とその応用
八田 秀雄; 北岡 祐; 星野 太佑
日本学術振興会, 科学研究費助成事業 基盤研究(B), 東京大学, 基盤研究(B), 昨年度、運動時の乳酸産生量を血中乳酸濃度から算出する方法を確立し、持久的トレーニングを実施したマウスに適用したところ、乳酸産生量として妥当な結果が得られた。そこで、今年度は運動時の血中乳酸濃度が低いことが明らかになっているperoxisome proliferator-activated receptor γ coactivator-1 (PGC-1)αの過剰発現マウスにおいて運動中の乳酸産生量を算出し、持久的トレーニングと比較・検討した。まず、PGC-1α過剰発現マウスの全身の乳酸取り込み能力は、野生型マウスと比較して有意に高いことがわかった。次に、絶対的な運動強度 (トレッドミルの速度)をあわせた運動中の乳酸産生量を算出したところ、PGC-1α過剰発現マウスの乳酸産生量は、野生型マウスと比較して有意に低値を示した。血中乳酸濃度が同等になるように相対的に運動強度を合わせた場合、PGC-1α過剰発現は乳酸産生量を増加させた。この結果は、全身の乳酸取り込み能力の高い動物が、コントロールの動物と同じ血中乳酸濃度を達成するためには、運動中より多くの乳酸産生量が必要であることを示唆している。また、重回帰分析により、持久的トレーニングを実施したマウスにおける乳酸産生量は、PFKとMCT4により正に、MCT1とミトコンドリア量により負に貢献していたが、PGC-1α過剰発現マウスにおける乳酸産生量には、そのような関係はみられなかった。このことから、PGC-1α過剰発現による乳酸産生量の抑制は、それらのタンパク質以外の影響が大きい可能性が示唆された。, 20H04071
01 Apr. 2020 - 31 Mar. 2024 - 持久的運動効果の減弱化のメカニズム解明とその解決策
星野 太佑
日本学術振興会, 科学研究費助成事業 基盤研究(C), 電気通信大学, 基盤研究(C), 昨年度，冷却はミトコンドリア生合成のマスターレギュレーターであるperoxisome proliferator-activated receptor γ coactivator-1 (PGC-1)αのmRNA発現量の筋収縮後の増加を減弱させることを明らかにした．今年度はその分子メカニズムについて，シグナル分子のリン酸化に着目し検証した． Wistar系雄性ラットを常温群と冷却群にわけ，常温群は水, 冷却群は氷水の入った袋を用いて，下腿前部を3分間冷却した. その後, 前脛骨筋を20 Hzにて1秒間電気刺激, 1秒間休息を30回繰り返すプロトコルを1セットとし, これを10セット実施した. 筋収縮直後に両脚の前脛骨筋を摘出し，AMP-activatedprotein kinase (AMPK) calcium/calmodulin-dependent protein kinase II (CaMKII)，p38 mitogen-activated protein kinase (p38 MAPK)のリン酸化をウエスタンブロッティングを用いて，測定した．また，筋グリコーゲン濃度と筋中乳酸濃度を生化学的に測定した．その結果，両群のAMPK，CaMKII，p38 MAPKのリン酸化は，筋収縮によって有意に増加した．さらに，冷却は，AMPKのリン酸化の増加を有意に減弱させた．しかし，CaMKII，p38 MAPKのリン酸化には，冷却の影響は見られなかった．また，筋グリコーゲン濃度の筋収縮による減少は，冷却により有意に抑制された． 以上の結果から，冷却は，筋収縮によるAMPKの活性化および筋グリコーゲン分解を減弱させ，PGC-1αのmRNA発現量の増加を抑制する可能性が示唆された．, 20K11417
01 Apr. 2020 - 31 Mar. 2023 - Effects of anti-doping drugs on metabolic adaptation
Hoshino Daisuke
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, The University of Electro-Communications, Grant-in-Aid for Early-Career Scientists, We examined the effects of clenbuterol administration and resistance training that induce muscle hypertrophy on metabolic adaptation in skeletal muscles and adipose tissues. Clenbuterol administration decreased the expression of proteins involved in mitochondrial fusion and fission, and increased abnormal morphological mitochondria in skeletal muscle. At the same time, there was no change in nuclear translocation of histone deacetylase 4 (HDAC4). Four-week resistance training reduced subcutaneous white adipocyte size and increased the protein levels of mitochondrial enzymes in brown adipose tissue. It is necessary to further clarify the mechanism of the above experimental results., 18K17792
01 Apr. 2018 - 31 Mar. 2020 - Elucidation of the mechanisms of skeletal muscle mitochondrial biogenesis by lactate
Hoshino Daisuke
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, The University of Tokyo, Grant-in-Aid for Young Scientists (B), Principal investigator, We examined whether lactate causes skeletal muscle mitochondrial biogenesis, using extracted mouse muscle incubation system and electric stimulation system to C2C12 myotubes. As a result, 24h-lactate stimulation (10 mM) for extracted mouse muscle suppressed mRNA contents of PGC-1alpha and mitochondria. However lactate concentration exceeded 25 mM after 24 hours of lactate incubation, which was non-physiological high lactate concentration. Elevation in lactate concentration due to electrical stimulation increased the mRNA contents of PGC-1alpha in C2C12 myotubes. These results suggest that the increase in lactate concentration associated with muscle contraction may cause mitochondrial biogenesis via transcriptional regulation. It is necessary to clarify the dose response of lactate concentration in further studies., 25750331
2013 - 2015 - 骨格筋の適応を引き起こす筋収縮の時間と強度の最適パターンの解明
星野太佑
文部科学省, 特別研究員奨励費, Principal investigator
Apr. 2014 - 運動における小脳の乳酸輸送単体monocarboxylate transporter2(MCT2)の役割の解明
星野太佑
日本科学協会, 笹川科学研究助成, Principal investigator
Apr. 2013 - Mar. 2014 - Potential role of lactate in exercise-induced mitochondrial biogenesis
HOSHINO Daisuke
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, The University of Tokyo, Grant-in-Aid for Research Activity Start-up, Principal investigator, We examined whether lactate accumulation associate with exercise-induced mitochondrial biogenesis. We found that dichloroacetate (DCA) injection can decrease muscle and plasma lactate concentration during exercise. DCA injection and exercise were performed in mice for 4 weeks. As a result, the decrease in lactate accumulation by DCA administration attenuated increases in mitochondrial proteins with exercise training. This result suggests that lactate can be a signal for exercise-induced mitochondrial biogenesis in skeletal muscle., 23800021
2011 - 2012
